1887

Abstract

A Gram-stain-negative, singly flagellated, aerobic and coccoid, ovoid or rod-shaped bacterium, designated strain JDTF-33, was isolated from a tidal flat in Jindo, an island of South Korea. Strain JDTF-33 grew optimally at 30 °C, at pH 7.0–8.0 and in the presence of 2.0–3.0 % (w/v) NaCl. A neighbour-joining phylogenetic tree, based on 16S rRNA gene sequences, demonstrated that strain JDTF-33 belonged to the genus , joining the type strain of the species with 97.9 % sequence similarity. Strain JDTF-33 exhibited 16S rRNA gene sequence similarities of 97.1 and 97.0 % to the type strains of and , respectively, and of 96.1–96.6 % to the type strains of the other species of the genus . Strain JDTF-33 showed DNA–DNA relatedness values of 11–24 % to the type strains of the species , and . Strain JDTF-33 contained Q-8 as the predominant ubiquinone and summed feature 3 (Cω7 and/or Cω6) and C as the major fatty acids. The major polar lipids detected in strain JDTF-33 were phosphatidylcholine, phosphatidylglycerol and phosphatidylethanolamine. The DNA G+C content of strain JDTF-33 was 41.7 mol%. Differential phenotypic properties, together with phylogenetic and genetic data, demonstrate that strain JDTF-33 is separate from species of the genus . On the basis of the data presented, strain JDTF-33 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is JDTF-33 (=KCTC 52838=NBRC 112782).

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2017-11-01
2019-12-14
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References

  1. Shivaji S, Reddy GS. Phylogenetic analyses of the genus Glaciecola: emended description of the genus Glaciecola, transfer of Glaciecola mesophila, G. agarilytica, G. aquimarina, G. arctica, G. chathamensis, G. polaris and G. psychrophila to the genus Paraglaciecola gen. nov. as Paraglaciecola mesophila comb. nov., P. agarilytica comb. nov., P. aquimarina comb. nov., P. arctica comb. nov., P. chathamensis comb. nov., P. polaris comb. nov. and P. psychrophila comb. nov., and description of Paraglaciecola oceanifecundans sp. nov., isolated from the Southern Ocean. Int J Syst Evol Microbiol 2014;64:3264–3275 [CrossRef][PubMed]
    [Google Scholar]
  2. Parte AC. LPSN–list of prokaryotic names with standing in nomenclature. Nucleic Acids Res 2014;42:D613–D616 [CrossRef][PubMed]
    [Google Scholar]
  3. Romanenko LA, Zhukova NV, Rohde M, Lysenko AM, Mikhailov VV et al. Glaciecola mesophila sp. nov., a novel marine agar-digesting bacterium. Int J Syst Evol Microbiol 2003;53:647–651 [CrossRef][PubMed]
    [Google Scholar]
  4. Van Trappen S, Tan TL, Yang J, Mergaert J, Swings J. Glaciecola polaris sp. nov., a novel budding and prosthecate bacterium from the Arctic Ocean, and emended description of the genus Glaciecola. Int J Syst Evol Microbiol 2004;54:1765–1771 [CrossRef][PubMed]
    [Google Scholar]
  5. Zhang DC, Yu Y, Chen B, Wang HX, Liu HC et al. Glaciecola psychrophila sp. nov., a novel psychrophilic bacterium isolated from the Arctic. Int J Syst Evol Microbiol 2006;56:2867–2869 [CrossRef][PubMed]
    [Google Scholar]
  6. Zhang YJ, Zhang XY, Mi ZH, Chen CX, Gao ZM et al. Glaciecola arctica sp. nov., isolated from Arctic marine sediment. Int J Syst Evol Microbiol 2011;61:2338–2341 [CrossRef][PubMed]
    [Google Scholar]
  7. Yong JJ, Park SJ, Kim HJ, Rhee SK. Glaciecola agarilytica sp. nov., an agar-digesting marine bacterium from the East Sea, Korea. Int J Syst Evol Microbiol 2007;57:951–953 [CrossRef][PubMed]
    [Google Scholar]
  8. Matsuyama H, Hirabayashi T, Kasahara H, Minami H, Hoshino T et al. Glaciecola chathamensis sp. nov., a novel marine polysaccharide-producing bacterium. Int J Syst Evol Microbiol 2006;56:2883–2886 [CrossRef][PubMed]
    [Google Scholar]
  9. Park S, Yoon JH. Glaciecola aquimarina sp. nov., a gammaproteobacterium isolated from coastal seawater. Antonie van Leeuwenhoek 2013;103:1141–1148 [CrossRef][PubMed]
    [Google Scholar]
  10. Park S, Won SM, Kim H, Park DS, Yoon JH. Aestuariivita boseongensis gen. nov., sp. nov., isolated from a tidal flat sediment. Int J Syst Evol Microbiol 2014;64:2969–2974 [CrossRef][PubMed]
    [Google Scholar]
  11. Lányí B. Classical and rapid identification methods for medically important bacteria. Methods Microbiol 1987;19:1–67
    [Google Scholar]
  12. Barrow GI, Feltham RKA. Cowan and Steel’s Manual for the Identification of Medical Bacteria, 3rd ed. Cambridge: Cambridge University Press; 1993;[Crossref]
    [Google Scholar]
  13. Bruns A, Rohde M, Berthe-Corti L. Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. Int J Syst Evol Microbiol 2001;51:1997–2006 [CrossRef][PubMed]
    [Google Scholar]
  14. Leifson E. Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 1963;85:1183–1184[PubMed]
    [Google Scholar]
  15. Yoon JH, Kim H, Kim SB, Kim HJ, Kim WY et al. Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol 1996;46:502–505 [CrossRef]
    [Google Scholar]
  16. Yoon JH, Lee ST, Kim S-B, Kim WY, Goodfellow M et al. Restriction fragment length polymorphism analysis of PCR-amplified 16S ribosomal DNA for rapid identification of Saccharomonospora strains. Int J Syst Bacteriol 1997;47:111–114 [CrossRef]
    [Google Scholar]
  17. Yoon JH, Kim IG, Shin DY, Kang KH, Park YH. Microbulbifer salipaludis sp. nov., a moderate halophile isolated from a Korean salt marsh. Int J Syst Evol Microbiol 2003;53:53–57 [CrossRef][PubMed]
    [Google Scholar]
  18. Ezaki T, Hashimoto Y, Yabuuchi E. Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 1989;39:224–229 [CrossRef]
    [Google Scholar]
  19. Komagata K, Suzuki K. Lipids and cell-wall analysis in bacterial systematics. Methods Microbiol 1987;19:161–207[Crossref]
    [Google Scholar]
  20. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101. Newark, DE: MIDI Inc; 1990
    [Google Scholar]
  21. Minnikin DE, O'Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984;2:233–241 [CrossRef]
    [Google Scholar]
  22. Embley TM, Wait R. Structural lipids of eubacteria. In Goodfellow M, O’Donnell AG. (editors) Modern Microbial Methods. Chemical Methods in Prokaryotic Systematics Chichester: John Wiley & Sons; 1994; pp.121–161
    [Google Scholar]
  23. Tamaoka J, Komagata K. Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 1984;25:125–128 [CrossRef]
    [Google Scholar]
  24. Stackebrandt E, Goebel BM. Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Evol Microbiol 1994;44:846–849 [CrossRef]
    [Google Scholar]
  25. Wayne LG, Moore WEC, Stackebrandt E, Kandler O, Colwell RR et al. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Evol Microbiol 1987;37:463–464 [CrossRef]
    [Google Scholar]
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