Two facultative chemolithotrophic, nitrate-reducing thiosulfate-oxidizing strains, F43bT and F21, were isolated from the sulfide-rich anoxic sediment of an urban creek in Pearl River Delta, China. Both strains were Gram-negative, facultatively anaerobic, non-spore-forming and rod-shaped with a flagellum. Phylogenetic analyses of 16S rRNA genes and the thrC, recA, glnII and atpD housekeeping genes revealed that the type strain shared high sequence similarities to Ciceribacter lividus MSSRFBL1T, with 98.8, 90.9, 94.8, 95.4 and 96.1 % identity, respectively. In addition, the major isoprenoid quinone (ubiquinone Q-10) and the DNA G+C content (66.0 mol%) of the type strain were similar to those of Ciceribacter lividus MSSRFBL1T. These results strongly support the classification of strains F43bT and F21 into the genus Ciceribacter. However, these strains diverged markedly from strain MSSRFBL1T with respect to several physiological and biochemical properties such as their semi-translucent colonies and nitrate-reducing and simultaneous thiosulfate-oxidizing respiration. Furthermore, the predominant fatty acids of strain F43bT were summed feature 2 (C18 : 1ω9t and/or C18 : 1ω9c and/or C18 : 1ω11t), C14 : 0 3-OH, C18 : 0 and C16 : 0, and its polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidymonomethylethanolamine and an unidentified glycolipid, which represented another two significant differences from strain MSSRFBL1T. Importantly, the DNA–DNA relatedness between strain F43bT and MSSRFBL1T was only 47.7 %. Based on the aforementioned polyphasic taxonomic results, the two isolates are suggested to represent a novel species of the genus Ciceribacter, for which the name Ciceribacterthiooxidans sp. nov. is proposed; the type strain is F43bT (=CCTCC AB 2016062T=KCTC 52231T).
KathiravanR,
JeganS,
GangaV,
PrabavathyVR,
TusharL et al.Ciceribacter lividus gen. nov., sp. nov., isolated from rhizosphere soil of chick pea (Cicer arietinum L.). Int J Syst Evol Microbiol2013; 63:4484–4488 [View Article][PubMed]
HeZ,
LongX,
LiL,
YuG,
ChongY et al. Temperature response of sulfide/ferrous oxidation and microbial community in anoxic sediments treated with calcium nitrate addition. J Environ Manage2017; 191:209–218 [View Article][PubMed]
MaYL,
YangBL,
ZhaoJL.
Removal of H2S by Thiobacillus denitrificans immobilized on different matrices. Bioresour Technol2006; 97:2041–2046 [View Article][PubMed]
KumarM,
VermaM,
LalR.
Devosia chinhatensis sp. nov., isolated from a hexachlorocyclohexane (HCH) dump site in India. Int J Syst Evol Microbiol2008; 58:861–865 [View Article][PubMed]
MartensM,
DelaereM,
CoopmanR,
de VosP,
GillisM et al. Multilocus sequence analysis of Ensifer and related taxa. Int J Syst Evol Microbiol2007; 57:489–503 [View Article][PubMed]
VinuesaP,
SilvaC,
WernerD,
Martínez-RomeroE.
Population genetics and phylogenetic inference in bacterial molecular systematics: the roles of migration and recombination in Bradyrhizobium species cohesion and delineation. Mol Phylogenet Evol2005; 34:29–54 [View Article][PubMed]
PolyF,
MonrozierLJ,
BallyR.
Improvement in the RFLP procedure for studying the diversity of nifH genes in communities of nitrogen fixers in soil. Res Microbiol2001; 152:95–103 [View Article][PubMed]
HaukkaK,
LindströmK,
YoungJP.
Three phylogenetic groups of nodA and nifH genes in Sinorhizobium and Mesorhizobium isolates from leguminous trees growing in Africa and Latin America. Appl Environ Microbiol1998; 64:419–426[PubMed]
NishijimaM,
Araki-SakaiM,
SanoH.
Identification of isoprenoid quinones by frit-FAB liquid chromatography–mass spectrometry for the chemotaxonomy of microorganisms. J Microbiol Methods1997; 28:113–122 [View Article]
MooreDD,
DowhanD.
Preparation and analysis of DNA. P.2-11. In
AusubelFW,
BrentR,
KingstonRE,
MooreDD,
SeidmanJG.
et al.
(editors) Current Protocols in Molecular Biology New York: Wiley; 1995
KaurJ,
VermaM,
LalR.
Rhizobium rosettiformans sp. nov., isolated from a hexachlorocyclohexane dump site, and reclassification of Blastobacter aggregatus Hirsch and Muller 1986 as Rhizobium aggregatum comb. nov. Int J Syst Evol Microbiol2011; 61:1218–1225 [View Article][PubMed]
LyL,
ChenWF,
HlL,
WangET,
ChenWX et al. isolated from caragana intermedia growing in saline-alkaline soils in the north of china. Int J Syst Evol Microbiol2009; 59:3006–3011[Crossref]