1887

Abstract

Two facultative chemolithotrophic, nitrate-reducing thiosulfate-oxidizing strains, F43b and F21, were isolated from the sulfide-rich anoxic sediment of an urban creek in Pearl River Delta, China. Both strains were Gram-negative, facultatively anaerobic, non-spore-forming and rod-shaped with a flagellum. Phylogenetic analyses of 16S rRNA genes and the thrC, recA, glnII and atpD housekeeping genes revealed that the type strain shared high sequence similarities to Ciceribacter lividus MSSRFBL1, with 98.8, 90.9, 94.8, 95.4 and 96.1 % identity, respectively. In addition, the major isoprenoid quinone (ubiquinone Q-10) and the DNA G+C content (66.0 mol%) of the type strain were similar to those of Ciceribacter lividus MSSRFBL1. These results strongly support the classification of strains F43b and F21 into the genus Ciceribacter . However, these strains diverged markedly from strain MSSRFBL1 with respect to several physiological and biochemical properties such as their semi-translucent colonies and nitrate-reducing and simultaneous thiosulfate-oxidizing respiration. Furthermore, the predominant fatty acids of strain F43b were summed feature 2 (C18 : 1ω9t and/or C18 : 1ω9c and/or C18 : 1ω11t), C14 : 0 3-OH, C18 : 0 and C16 : 0, and its polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidymonomethylethanolamine and an unidentified glycolipid, which represented another two significant differences from strain MSSRFBL1. Importantly, the DNA–DNA relatedness between strain F43b and MSSRFBL1 was only 47.7 %. Based on the aforementioned polyphasic taxonomic results, the two isolates are suggested to represent a novel species of the genus Ciceribacter , for which the name Ciceribacter thiooxidans sp. nov. is proposed; the type strain is F43b (=CCTCC AB 2016062=KCTC 52231).

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2017-10-06
2019-10-15
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