RT Journal Article SR Electronic(1) A1 Ryzhmanova, Yana A1 Oshurkova, Victoria A1 Troshina, Olga A1 Abashina, Tatyana A1 Ariskina, Elena A1 Avtukh, Alexander A1 Shcherbakova, ViktoriaYR 2017 T1 Anoxynatronum buryatiense sp. nov., an anaerobic alkaliphilic bacterium from a low mineralization soda lake in Buryatia, Russia JF International Journal of Systematic and Evolutionary Microbiology, VO 67 IS 11 SP 4704 OP 4709 DO https://doi.org/10.1099/ijsem.0.002365 PB Microbiology Society, SN 1466-5034, AB An anaerobic alkaliphilic, proteolytic bacterium, strain Su22T, was isolated from the bottom sediment of the alkaline low mineralization lake Sulphatnoe (Selenginsky district, Buryatia, Russia). A comparative analysis of the 16S rRNA gene sequence revealed that this bacterium was closely related to Anoxynatronum sibiricum Z-7981T with a similarity of 98.1 %. Strain Su22T differed from A. sibiricum Z-7981T in its inability to use carbohydrates, peptone and amino acids as carbon sources. Strain Su22T grew over a temperature range of 20–40 °C with an optimum at 30 °C and within the pH range 7.4–11.0 with an optimum at pH 9.6. Sodium cations stimulated the growth of the strain considerably with an optimal concentration at 0.76–1.09 M. The whole-cell fatty acid profile included C16 : 1ω7c, C16 : 0 and C16 : 0 ALDE. The G+C content was 46.1 mol%. Based on the DNA–DNA hybridization level (53.2 %) and phenotypical differences between strains Su22T and Z-7981T, the new isolate is thus considered to represent a novel species, for which the name Anoxynatronum buryatiense sp. nov. is proposed. The type strain is Su22Т (=VKM B-2510T=CECT 8731T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002365