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Volume 67, Issue 11

sp. nov., isolated from grassland soil Free

Abstract

A novel bacterial strain, designed Q4-3, was isolated from a soil sample obtained from Qilian grassland, Qinghai, China. Phylogenetic, phenotypic, chemotaxonomic and molecular analyses were performed on the new isolate. Cells were Gram-stain-positive, facultatively anaerobic, spore-forming, motile rods with peritrichous flagella. Phylogenetic analysis based on 16S rRNA gene sequences placed strain Q4-3 in the genus , and its closest relatives were JCM 21743, DSM 25190, DSM 13188 and DSM 29760 with 16S rRNA gene sequence similarities of 98.12, 97.89, 97.63 and 97.6 %, respectively. The isolate grew at 4-37 °C (optimum 28-30 °C), at pH 6.0-10.0 (optimum pH 7.5) and with 0-3 %(w/v) NaCl (optimum 1 %). The DNA of strain Q4-3 was determined to be 48.6 mol%. The predominant menaquinone was MK-7 and the diamino acid in the cell-wall peptidoglycan was found to be -diaminopimelic acid. Anteiso-C (55.5 %), iso-C (14.5 %) and C (13.3 %) were the major fatty acids. The polar lipids contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, three unidentified aminophospholipids and one unidentified lipid. Based on these results, strain Q4-3 is considered to represent a novel of the genus , for which the name nov. is proposed. The type strain is Q4-3 (=CGMCC 1.16134=KCTC 33911).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): 16S rRNA gene , new species , Paenibacillus and taxonomy
© 2017 IUMS
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2017-11-01
2024-04-19
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Paenibacillus albidus sp. nov., isolated from grassland soil
Int J Syst Evol Microbiol 67, 4685 (2017); https://doi.org/10.1099/ijsem.0.002356
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