RT Journal Article SR Electronic(1) A1 Li, Yong A1 Xue, Han A1 Guo, Li-min A1 Koltay, András A1 Palacio-Bielsa, Ana A1 Chang, Jupu A1 Xie, Shoujiang A1 Yang, XuqiYR 2017 T1 Elevation of three subspecies of Lonsdalea quercina to species level: Lonsdalea britannica sp. nov., Lonsdalea iberica sp. nov. and Lonsdalea populi sp. nov. JF International Journal of Systematic and Evolutionary Microbiology, VO 67 IS 11 SP 4680 OP 4684 DO https://doi.org/10.1099/ijsem.0.002353 PB Microbiology Society, SN 1466-5034, AB Four subspecies of Lonsdalea quercina ( L. quercina subsp. quercina , L. quercina subsp. britannica , L. quercina subsp. iberica and L. quercina subsp. populi ) were studied by genome sequence-derived average nucleotide identity (ANI), phylogenetic analysis based on 16S rRNA gene sequences, multilocus sequence analysis (MLSA) and phenotypic characteristics. In phylogenetic trees, based on 16S rRNA gene sequences, and in MLSA data, the four subspecies were divided into four subclusters in the Lonsdalea clade with high boot strap support. The ANI values between the four subspecies were 88.71–93.38 %, respectively, lower than the proposed species boundary ANI cut-off (95–96 %) that is considered the most important criterion to reclassify these subspecies at the species level. It is proposed that three subspecies be elevated to the species level as Lonsdalea britannica sp. nov. (type strain R-43280T=LMG 26267T=NCPPB 4481T=CFCC 10822T), Lonsdalea iberica sp. nov. (type strain R-44166T=LMG 26264T=NCPPB 4490T=CFCC 10824T) and Lonsdalea populi sp. nov. (type strain NY060T=DSM 25466T=NCAIM B 02483T=LMG 27349T=CFCC 13125T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002353