RT Journal Article SR Electronic(1) A1 Zhao, Junwei A1 Li, Wenchao A1 Shi, Linlin A1 Wang, Han A1 Wang, Ying A1 Zhao, Yue A1 Xiang, Wensheng A1 Wang, XiangjingYR 2017 T1 Lechevalieria rhizosphaerae sp. nov., a novel actinomycete isolated from rhizosphere soil of wheat (Triticum aestivum L.) and emended description of the genus Lechevalieria JF International Journal of Systematic and Evolutionary Microbiology, VO 67 IS 11 SP 4655 OP 4659 DO https://doi.org/10.1099/ijsem.0.002351 PB Microbiology Society, SN 1466-5034, AB A novel actinomycete, designated strain NEAU-A2T, was isolated from rhizosphere soil of wheat (Triticum aestivum L.) and characterized using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain NEAU-A2T should be assigned to the genus Lechevalieria and forms a distinct branch with its closest neighbour Lechevalieria aerocolonigenes DSM 40034T (99.0 %). Moreover, key morphological and chemotaxonomic properties also confirmed the affiliation of strain NEAU-A2T to the genus Lechevalieria . The cell wall contained meso-diaminopimelic acid and the whole-cell hydrolysates were galactose, mannose, rhamnose, glucose and ribose. The polar lipids were diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylinositolmannoside and two glycolipids. The predominant menaquinones were MK-9(H4) and MK-9(H6). The major fatty acids were iso-C16 : 0, anteiso-C15 : 0, C16 : 1ω7c and anteiso-C17 : 0. The DNA G+C content was 68.2 mol%. The combination of the DNA–DNA hybridization result and some phenotypic characteristics demonstrated that strain NEAU-A2T could be distinguished from its closest relative. Therefore, it is proposed that strain NEAU-A2T represents a novel species of the genus Lechevalieria , for which the name Lechevalieria rhizosphaerae sp. nov. is proposed. The type strain is NEAU-A2T (=CGMCC 4.7405T=DSM 104541T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002351