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Abstract

Strain D-14, a brown-coloured, Gram-stain-negative, non-motile and rod-shaped bacterium, was isolated from oil-contaminated soil. It was able to grow at 20–40 °C, at pH 6.0–10.0 and at 0–1 % (w/v) NaCl concentration. Based on the 16S rRNA gene sequence analysis, strain D-14 belonged to the genus and was closely related to BUT-8 (99.0 % sequence similarity), CTN-1 (98.5 %), GH1-9 (98.2 %) and CJ29 (97.2 %). The only respiratory quinone was ubiquinone-8. The polar lipid profile revealed the presence of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and phosphatidyl--methyl-ethanolamine. The predominant fatty acids of strain D-14 were iso-C, iso-C, summed feature 9 (iso-C ω9 and/or C 10-methyl), summed feature 3 (C ω7 and/or C ω6), iso-C, Ciso 3-OH, Ciso F and C. The genomic DNA G+C content of this novel strain was 68.7 mol%. The DNA–DNA relatedness values between strain D-14 and BUT-8, CTN-1, GH1-9 and CJ29 were 56.0, 46.3, 48.7 and 41.7 %, respectively, which fall below the threshold value of 70 % for the strain to be considered as novel. The morphological, physiological, chemotaxonomic and phylogenetic analyses clearly distinguished this strain from its closest phylogenetic neighbours. Thus, strain D-14 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is D-14 (=KEMB 9005-572=KACC 19173=JCM 31917).

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2017-11-01
2020-01-28
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