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Abstract

A novel bacterium, designated as strain HJR7, was isolated from a marine sediment sample collected from the coastal area of Weihai, China (121° 57′ E, 37° 29′ N). Cells were Gram-stain-negative, facultative anaerobic, non-motile and rod-shaped. The temperature, pH and NaCl ranges for growth were determined as 4–40 °C, pH 6.5–9.5 and 0.5–15.0 % (w/v), respectively. Phylogenetic analysis based on the 16S rRNA gene sequences indicated that strain HJR7 belongs to the genus in the family . The most closely related species were (97.6 % 16S rRNA gene sequence similarity) and (97.3 % similarity). Ubiquinone 9 (Q-9) was the only respiratory quinone detected in strain HJR7. The major fatty acids of strain HJR7 were C C C N alcohol, Cω and Cω6, 9, 12. The major polar lipids were phosphatidylglycerol, phosphatidylethanolamine, and an unidentified phospholipid. The DNA G+C content of strain HJR7 was 53.7 mol%. On the basis of phylogenetic, genotypic, phenotypic, and chemotaxonomic analyses, strain HJR7 represents a novel species within the genus , for which the name sp. nov. is proposed. The type strain is HJR7 (=KCTC 52545=MCCC 1H00176).

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2017-11-01
2020-01-17
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References

  1. Gauthier MJ, Lafay B, Christen R, Fernandez L, Acquaviva M et al. Marinobacter hydrocarbonoclasticus gen. nov., sp. nov., a new, extremely halotolerant, hydrocarbon-degrading marine bacterium. Int J Syst Bacteriol 1992;42:568–576 [CrossRef][PubMed]
    [Google Scholar]
  2. Vaidya B, Kumar R, Korpole S, Tanuku NR, Pinnaka AK et al. Marinobacter nitratireducens sp. nov., a halophilic and lipolytic bacterium isolated from coastal surface sea water. Int J Syst Evol Microbiol 2015;65:2056–2063 [CrossRef][PubMed]
    [Google Scholar]
  3. Kim BY, Weon HY, Yoo SH, Kim JS, Kwon SW et al. Marinobacter koreensis sp. nov., isolated from sea sand in Korea. Int J Syst Evol Microbiol 2006;56:2653–2656 [CrossRef][PubMed]
    [Google Scholar]
  4. Cui Z, Gao W, Xu G, Luan X, Li Q et al. Marinobacter aromaticivorans sp. nov., a polycyclic aromatic hydrocarbon-degrading bacterium isolated from sea sediment. Int J Syst Evol Microbiol 2016;66:353–359 [CrossRef][PubMed]
    [Google Scholar]
  5. Kharroub K, Aguilera M, Jiménez-Pranteda ML, González-Paredes A, Ramos-Cormenzana A et al. Marinobacter oulmenensis sp. nov., a moderately halophilic bacterium isolated from brine of a salt concentrator. Int J Syst Evol Microbiol 2011;61:2210–2214 [CrossRef][PubMed]
    [Google Scholar]
  6. Zhong ZP, Liu Y, Liu HC, Wang F, Zhou YG et al. Marinobacter halophilus sp. nov., a halophilic bacterium isolated from a salt lake. Int J Syst Evol Microbiol 2015;65:2838–2845 [CrossRef][PubMed]
    [Google Scholar]
  7. Shieh WY, Jean WD, Lin YT, Tseng M. Marinobacter lutaoensis sp. nov., a thermotolerant marine bacterium isolated from a coastal hot spring in Lutao, Taiwan. Can J Microbiol 2003;49:244–252 [CrossRef][PubMed]
    [Google Scholar]
  8. Luo YJ, Xie BS, Lv XL, Cai M, Wang YN et al. Marinobacter shengliensis sp. nov., a moderately halophilic bacterium isolated from oil-contaminated saline soil. Antonie van Leeuwenhoek 2015;107:1085–1094 [CrossRef][PubMed]
    [Google Scholar]
  9. Yoon JH, Lee MH, Kang SJ, Oh TK. Marinobacter salicampi sp. nov., isolated from a marine solar saltern in Korea. Int J Syst Evol Microbiol 2007;57:2102–2105 [CrossRef][PubMed]
    [Google Scholar]
  10. Lee OO, Lai PY, Wu HX, Zhou XJ, Miao L et al. Marinobacter xestospongiae sp. nov., isolated from the marine sponge Xestospongia testudinaria collected from the Red Sea. Int J Syst Evol Microbiol 2012;62:1980–1985 [CrossRef][PubMed]
    [Google Scholar]
  11. Liebgott PP, Casalot L, Paillard S, Lorquin J, Labat M. Marinobacter vinifirmus sp. nov., a moderately halophilic bacterium isolated from a wine-barrel-decalcification wastewater. Int J Syst Evol Microbiol 2006;56:2511–2516 [CrossRef][PubMed]
    [Google Scholar]
  12. Montes MJ, Bozal N, Mercadé E. Marinobacter guineae sp. nov., a novel moderately halophilic bacterium from an Antarctic environment. Int J Syst Evol Microbiol 2008;58:1346–1349 [CrossRef][PubMed]
    [Google Scholar]
  13. Park S, Kim S, Kang CH, Jung YT, Yoon JH. Marinobacter confluentis sp. nov., a lipolytic bacterium isolated from a junction between the ocean and a freshwater lake. Int J Syst Evol Microbiol 2015;65:4873–4879 [CrossRef][PubMed]
    [Google Scholar]
  14. Antunes A, França L, Rainey FA, Huber R, Nobre MF et al. Marinobacter salsuginis sp. nov., isolated from the brine-seawater interface of the Shaban Deep, Red Sea. Int J Syst Evol Microbiol 2007;57:1035–1040 [CrossRef][PubMed]
    [Google Scholar]
  15. Green DH, Bowman JP, Smith EA, Gutierrez T, Bolch CJ. Marinobacter algicola sp. nov., isolated from laboratory cultures of paralytic shellfish toxin-producing dinoflagellates. Int J Syst Evol Microbiol 2006;56:523–527 [CrossRef][PubMed]
    [Google Scholar]
  16. Mesbah M, Premachandran U, Whitman WB. Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 1989;39:159–167 [CrossRef]
    [Google Scholar]
  17. Liu QQ, Wang Y, Li J, Du ZJ, Chen GJ. Saccharicrinis carchari sp. nov., isolated from a shark, and emended descriptions of the genus Saccharicrinis and Saccharicrinis fermentans. Int J Syst Evol Microbiol 2014;64:2204–2209 [CrossRef][PubMed]
    [Google Scholar]
  18. Yoon SH, Ha SM, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017;67:1613–1617 [CrossRef][PubMed]
    [Google Scholar]
  19. Ludwig W, Strunk O, Westram R, Richter L, Meier H et al. ARB: a software environment for sequence data. Nucleic Acids Res 2004;32:1363–1371 [CrossRef][PubMed]
    [Google Scholar]
  20. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985;39:783–791 [CrossRef][PubMed]
    [Google Scholar]
  21. Lowe TM, Eddy SR. tRNAscan-SE: a program for improved detection of transfer RNA genes in genomic sequence. Nucleic Acids Res 1997;25:955–964 [CrossRef][PubMed]
    [Google Scholar]
  22. Lagesen K, Hallin P, Rødland EA, Staerfeldt HH, Rognes T et al. RNAmmer: consistent and rapid annotation of ribosomal RNA genes. Nucleic Acids Res 2007;35:3100–3108 [CrossRef][PubMed]
    [Google Scholar]
  23. Medema MH, Blin K, Cimermancic P, de Jager V, Zakrzewski P et al. antiSMASH: rapid identification, annotation and analysis of secondary metabolite biosynthesis gene clusters in bacterial and fungal genome sequences. Nucleic Acids Res 2011;39:W339–W346 [CrossRef][PubMed]
    [Google Scholar]
  24. Richter M, Rosselló-Móra R. Shifting the genomic gold standard for the prokaryotic species definition. Proc Natl Acad Sci USA 2009;106:19126–19131 [CrossRef][PubMed]
    [Google Scholar]
  25. Auch AF, von Jan M, Klenk HP, Göker M. Digital DNA-DNA hybridization for microbial species delineation by means of genome-to-genome sequence comparison. Stand Genomic Sci 2010;2:117–134 [CrossRef][PubMed]
    [Google Scholar]
  26. Wayne LG, Brenner DJ, Colwell RR, Grimont PAD, Kandler O et al. International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 1987;37:463–464[Crossref]
    [Google Scholar]
  27. Bowman JP. Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov. Int J Syst Evol Microbiol 2000;50:1861–1868 [CrossRef][PubMed]
    [Google Scholar]
  28. Dong XZ, Cai MY. (editors) Chapter 14. Determination of biochemical characteristics. In Manual for the Systematic Identification of General Bacteria Beijing, China: Science Press; 2001; pp.370–398
    [Google Scholar]
  29. Mata JA, Martínez-Cánovas J, Quesada E, Béjar V. A detailed phenotypic characterisation of the type strains of Halomonas species. Syst Appl Microbiol 2002;25:360–375 [CrossRef][PubMed]
    [Google Scholar]
  30. Gordon RE, Smith MM. Rapidly growing, acid fast bacteria. II. Species' description of Mycobacterium fortuitum Cruz. J Bacteriol 1955;69:502–507[PubMed]
    [Google Scholar]
  31. Jorgensen JH, Turnidge JD. Antibacterial susceptibility tests: dilution and disk diffusion methods. In: Manual of clinical microbiology 2007
    [Google Scholar]
  32. Tindall BJ. Lipid composition of Halobacterium lacusprofundi. FEMS Microbiol Lett 1990;66:199–202 [CrossRef]
    [Google Scholar]
  33. Tindall BJ. A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. Syst Appl Microbiol 1990;13:128–130 [CrossRef]
    [Google Scholar]
  34. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids MIDI Technical Note 101 Newark, DE: MIDI; 1990
    [Google Scholar]
  35. Bligh EG, Dyer WJ. A rapid method of total lipid extraction and purification. Can J Biochem Physiol 1959;37:911–917 [CrossRef][PubMed]
    [Google Scholar]
  36. Tindall BJ, Sikorski J, Smibert RM, Kreig NR. Phenotypic characterization and the principles of comparative systematics. In Methods for General and Molecular Microbiology, 3rd ed. Washington, DC: ASM Press; 2007; pp.330–393
    [Google Scholar]
  37. Shivaji S, Gupta P, Chaturvedi P, Suresh K, Delille D. Marinobacter maritimus sp. nov., a psychrotolerant strain isolated from sea water off the subantarctic Kerguelen islands. Int J Syst Evol Microbiol 2005;55:1453–1456 [CrossRef][PubMed]
    [Google Scholar]
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