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Volume 67, Issue 11

gen. nov., sp. nov., a novel anaerobic bacterium isolated from a hot spring Free

Abstract

A novel Gram-stain-positive, obligately anaerobic and thermophilic bacterium, strain SYSU GA15001, was isolated from a sample collected from a hot spring located in Tengchong, China. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain SYSU GA15001 belonged to the family , and was closely related to ‘’ 2PG-426-CC4.2 (93.6 % similarity), DSM 15102 (93.2 %) and Z-78920 (89.7 %). Cells were rods and motile by means of a subpolar flagellum. Spores were formed in old cultures. Optimum growth was observed at 45 °C, at pH 7 and in the presence of 1.5 % (w/v) NaCl. The strain utilized -arabinose, dulcitol, -galactose, glucose, lactose, maltose, -mannose, raffinose, -rhamnose, -sorbitol, xylitol, -alanine, -arginine, glycine, -isoleucine, -leucine, -methionine, NHCl, -proline and -threonine as sole carbon/nitrogen sources. Additionally, complex substrates including beef extract, peptone, starch and yeast extract can also be used as sole energy sources. The major fatty acid methyl esters were C, anteiso-C and C. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, an unidentified polar lipid, three unidentified phospholipids and an unidentified glycolipid. The DNA GC content was determined to be 35.6 mol%. Strain SYSU GA15001 was physiologically and phylogenetically distinguishable from its closely related genera, and merits assignment as representing the type species of a new genus , with the name gen. nov., sp. nov. The type strain of the type species is SYSU GA15001 (=CGMCC 1.5219=KCTC 15623).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): hot spring , polyphasic taxonomy and Tengchong
© 2017 IUMS
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2017-11-01
2024-04-25
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Rhabdanaerobium thermarum gen. nov., sp. nov., a novel anaerobic bacterium isolated from a hot spring
Int J Syst Evol Microbiol 67, 4584 (2017); https://doi.org/10.1099/ijsem.0.002335
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