1887

Abstract

An aerobic facultatively methylotrophic bacterium was isolated from roots of L. and designated strain Osot The cells of this strain were Gram-stain-negative, asporogenous, motile short rods multiplying by binary fisson. They utilized methanol, methylamines and a variety of polycarbon compounds as the carbon and energy sources. Methanol was assimilated after sequential oxidation to formaldehyde and CO via the ribulose bisphosphate pathway. The organism grew optimally at 22–29 °C and pH 7.5–8.0. The dominant phospholipids were phosphatidylethanolamine, phosphatidylcholine, phosphatidylglycerol and diphosphatidylglycerol (cardiolipin). The major cellular fatty acids of strain Osot cells grown in R2A medium were C 7 (49.0 %), C 8 cyclo (38.3 %) and C (8.4 %). The major ubiquinone was Q-10. The DNA G+C content of strain Osot was 66.1 mol% ( ). On the basis of 16S rRNA gene sequence analysis strain Osot is phylogenetically related to the members of genus (97.1–98.8 % sequence similarity). Based on 16S rRNA gene sequence analysis and DNA–DNA relatedness (27–29 %) with type strains of the genus , the novel isolate is classified as a new species of this genus and named sp. nov.; the type strain is Osot (=VKM B-3145=JCM 32039).

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2017-11-01
2022-01-19
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