1887

Abstract

A Gram-positive bacterial strain, 99221/2016, was isolated from blood of a patient with bacteraemia at the Institute of Medical Microbiology, Göttingen, Germany. The strain was rod-shaped with a palisade arrangement of cells, non-spore-forming, non-lipophilic, catalase-positive and oxidase-negative. It grew well at 37 °C on Columbia blood agar and showed good growth under aerobic, microaerophilic and anaerobic conditions. The colonies were white-cream, circular and convex with a shiny, smooth surface. The predominant respiratory quinones were MK-8(H) and MK-9(H). The polar lipids profile contained phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol. Two unidentified phospholipids and several unidentified lipids were also detected. The prevalent cellular fatty acids comprised -9-octadecenoic acid (C ω9), hexadecanoic acid (C) and pentadecanoic acid (C). Corynemycolates with 28–36 carbons in length were present. The whole-cell hydrolysate contained -diaminopimelic acid and arabinose, glucose, galactose and ribose as major sugars. Analysis of the 16S rRNA gene sequence identities revealed that the strain is most closely related to DSM 44264 (98.0 %), DSM 44291 (96.9 %), subsp. DSM 44280 (96.9 %) and subsp. DSM 44282 (96.8 %). The identity with DSM 44123, the type species of the genus, was 94 %. The DNA G+C content was 69.2 mol%. DNA–DNA hybridization with DSM 44264 revealed a value of 34 %, confirming that the strain represents a novel species. The type strain 99221/2016 (DSM 103494=JCM 31931) is proposed to represent a novel species of the genus with the name .

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.002322
2017-11-01
2020-11-24
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/67/11/4494.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.002322&mimeType=html&fmt=ahah

References

  1. Lehmann KB, Neumann R. Atlas und Grundriss der Bakteriologie und Lehrbuch der speciellen bakteriologischen Diagnostik, 1st ed. München: J.F. Lehmann; 1896
    [Google Scholar]
  2. Bernard KA, Wiebe D, Burdz T, Reimer A, Ng B et al. Assignment of Brevibacterium stationis (ZoBell and Upham 1944) Breed 1953 to the genus Corynebacterium, as Corynebacterium stationis comb. nov., and emended description of the genus Corynebacterium to include isolates that can alkalinize citrate. Int J Syst Evol Microbiol 2010;60:874–879 [CrossRef][PubMed]
    [Google Scholar]
  3. Euzéby JP. List of bacterial names with standing in nomenclature: a folder available on the Internet. Int J Syst Bacteriol 1997;47:590–592 [CrossRef][PubMed]
    [Google Scholar]
  4. Pitcher DG. Deoxyribonucleic acid base composition of Corynebacterium diphtheriae and other corynebacteria with cell wall type IV. FEMS Microbiol Lett 1983;16:291–295 [CrossRef]
    [Google Scholar]
  5. Funke G, Lawson PA, Collins MD. Heterogeneity within human-derived centers for disease control and prevention (CDC) coryneform group ANF-1-like bacteria and description of Corynebacterium auris sp. nov. Int J Syst Bacteriol 1995;45:735–739 [CrossRef][PubMed]
    [Google Scholar]
  6. Bernard KA, Funke G. Genus Corynebacterium Lehmann and Neumann 1896, 350AL. In Whitman W, Goodfellow M, Kämpfer P, Busse H-J, Trujillo ME et al. (editors) Bergey’s Manual of Systematic Bacteriology, 2nd ed.vol. 5 New York: Springer; 2012; pp.245–246
    [Google Scholar]
  7. Frischmann A, Knoll A, Hilbert F, Zasada AA, Kämpfer P et al. Corynebacterium epidermidicanis sp. nov., isolated from skin of a dog. Int J Syst Evol Microbiol 2012;62:2194–2200 [CrossRef][PubMed]
    [Google Scholar]
  8. Kämpfer P, Andersson MA, Rainey FA, Kroppenstedt RM, Salkinoja-Salonen M. Williamsia muralis gen. nov., sp. nov., isolated from the indoor environment of a children's day care centre. Int J Syst Bacteriol 1999;49:681–687 [CrossRef][PubMed]
    [Google Scholar]
  9. Kovacs N. Identification of Pseudomonas pyocyanea by the oxidase reaction. Nature 1956;178:703 [CrossRef][PubMed]
    [Google Scholar]
  10. Gerhardt P. Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology; 1994
    [Google Scholar]
  11. Kim OS, Cho YJ, Lee K, Yoon SH, Kim M et al. Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 2012;62:716–721 [CrossRef][PubMed]
    [Google Scholar]
  12. Yarza P, Ludwig W, Euzéby J, Amann R, Schleifer KH et al. Update of the All-Species Living Tree Project based on 16S and 23S rRNA sequence analyses. Syst Appl Microbiol 2010;33:291–299 [CrossRef][PubMed]
    [Google Scholar]
  13. Meier-Kolthoff JP, Auch AF, Klenk HP, Göker M. Genome sequence-based species delimitation with confidence intervals and improved distance functions. BMC Bioinformatics 2013;14:60 [CrossRef][PubMed]
    [Google Scholar]
  14. Meier-Kolthoff JP, Hahnke RL, Petersen J, Scheuner C, Michael V et al. Complete genome sequence of DSM 30083T, the type strain (U5/41T) of Escherichia coli, and a proposal for delineating subspecies in microbial taxonomy. Stand Genomic Sci 2014;9:2 [CrossRef][PubMed]
    [Google Scholar]
  15. Edgar RC. MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res 2004;32:1792–1797 [CrossRef][PubMed]
    [Google Scholar]
  16. Stamatakis A. RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics 2014;30:1312–1313 [CrossRef][PubMed]
    [Google Scholar]
  17. Goloboff PA, Farris JS, Nixon KC. TNT, a free program for phylogenetic analysis. Cladistics 2008;24:774–786 [CrossRef]
    [Google Scholar]
  18. Pattengale ND, Alipour M, Bininda-Emonds OR, Moret BM, Stamatakis A. How many bootstrap replicates are necessary?. J Comput Biol 2010;17:337–354 [CrossRef][PubMed]
    [Google Scholar]
  19. Swofford DL. PAUP*: Phylogenetic Analysis Using Parsimony (*and Other Methods), Version 4.0 b10 Sunderland: Sinauer Associates; 2002
    [Google Scholar]
  20. Cashion P, Holder-Franklin MA, McCully J, Franklin M. A rapid method for the base ratio determination of bacterial DNA. Anal Biochem 1977;81:461–466 [CrossRef][PubMed]
    [Google Scholar]
  21. De Ley J, Cattoir H, Reynaerts A. The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 1970;12:133–142 [CrossRef][PubMed]
    [Google Scholar]
  22. Huss VA, Festl H, Schleifer KH. Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 1983;4:184–192 [CrossRef][PubMed]
    [Google Scholar]
  23. Wayne LG, Brenner DJ, Colwell RR, Grimont PAD, Kandler O et al. International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Evol Microbiol 1987;37:463–464[Crossref]
    [Google Scholar]
  24. Mesbah M, Premachandran U, Whitman WB. Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 1989;39:159–167 [CrossRef]
    [Google Scholar]
  25. Bruce J. Automated system rapidly identifies and characterizes microorganisms in food. Food Tech 1996;50:77–81
    [Google Scholar]
  26. Schumann P, Pukall R. The discriminatory power of ribotyping as automatable technique for differentiation of bacteria. Syst Appl Microbiol 2013;36:369–375 [CrossRef][PubMed]
    [Google Scholar]
  27. Sasser M. Identification of bacteria by gas chromatography of cellular fatty acids. USFCC Newsletter 1990;20:1–6
    [Google Scholar]
  28. Kämpfer P, Kroppenstedt RM. Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 1996;42:989–1005 [CrossRef]
    [Google Scholar]
  29. Rhuland LE, Work E, Denman RF, Hoare DS. The behavior of the isomers of α,ε-diaminopimelic acid on paper chromatograms. J Am Chem Soc 1955;77:4844–4846 [CrossRef]
    [Google Scholar]
  30. Staneck JL, Roberts GD. Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 1974;28:226–231[PubMed]
    [Google Scholar]
  31. Bligh EG, Dyer WJ. A rapid method of total lipid extraction and purification. Can J Biochem Physiol 1959;37:911–917 [CrossRef][PubMed]
    [Google Scholar]
  32. Tindall BJ, Sikorski J, Smibert RM, Krieg NR. Phenotypic characterization and the principles of comparative systematics. In Reddy CA, Beveridge TJ, Breznak JA, Marzluf GA, Schmidt TM et al. (editors) Methods for General and Molecular Microbiology, 3rd ed. Washington, DC: ASM Press; 2007; pp.330–393
    [Google Scholar]
  33. Kröger A. Determination of contents and redox states of ubiquinone and menaquinone. Methods Enzymol 1978;53:579–591[PubMed][Crossref]
    [Google Scholar]
  34. Tindall BJ. A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. Syst Appl Microbiol 1990;13:128–130 [CrossRef]
    [Google Scholar]
  35. Tindall BJ. Lipid composition of Halobacterium lacusprofundi. FEMS Microbiol Lett 1990;66:199–202 [CrossRef]
    [Google Scholar]
  36. Kämpfer P, Lodders N, Warfolomeow I, Falsen E, Busse HJ. Corynebacterium lubricantis sp. nov., isolated from a coolant lubricant. Int J Syst Evol Microbiol 2009;59:1112–1115 [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.002322
Loading
/content/journal/ijsem/10.1099/ijsem.0.002322
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error