@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.002312, author = "Pan, Tong and He, Hairong and Wang, Xiaochong and Shen, Yibo and Zhao, Junwei and Yan, Kai and Wang, Xiangjing and Liu, Chongxi and Zhang, Ji and Xiang, Wensheng", title = "Bacillus solisilvae sp. nov., isolated from forest soil", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2017", volume = "67", number = "11", pages = "4449-4455", doi = "https://doi.org/10.1099/ijsem.0.002312", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002312", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "Changbai Mountain", keywords = "Bacillus solisilvae", abstract = "A novel Gram-stain-positive, motile, endospore-forming, rod-shaped bacterial strain, NEAU-cbsb5T, was isolated from forest soil from Changbai Mountain, Heilongjiang Province, China. The isolate grew at 15–40 °C (optimum 30 °C), at pH 6.0–8.0 (optimum pH 7.0) and in the presence of up to 4 % (w/v) NaCl, although NaCl was not required for growth. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain NEAU-cbsb5T formed a distinct lineage within the genus Bacillus and was most closely related to Bacillus acidiceler DSM 18954T (99.1 % similarity) and Bacillus luciferensis JCM 12212T (99.0 %). 16S rRNA gene sequence similarity to sequences of the type strains of other Bacillus species was less than 96.0 %. Average nucleotide identity (ANI) values between NEAU-cbsb5T and its most closely related species were 78.72–84.75 % by ANIm, ANIb and OrthoANIu analysis. The in silico DNA–DNA hybridization values between strain NEAU-cbsb5T and its close relatives B. acidiceler DSM 18954T and B. luciferensis JCM 12212T were both 23.80 %, again indicating they belong to different taxa. The major cellular fatty acids of NEAU-cbsb5T were iso-C15 : 0, anteiso-C15 : 0 and C16 : 0. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and an unknown aminophospholipid. The cell-wall peptidoglycan contained meso-diaminopimelic acid and the predominant menaquinones were MK-7 and MK-6. The genomic DNA G+C content was 33.0 mol%. Based on the phylogenetic, phenotypic and chemotaxonomic data, strain NEAU-cbsb5T was classified as a representative of a novel species in the genus Bacillus , for which the name Bacillus solisilvae sp. nov. is proposed. The type strain is NEAU-cbsb5T (=CGMCC 1.14993T=DSM 100485T).", }