Skip to content
1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 67, Issue 11

gen. nov., sp. nov., a prosthecate alphaproteobacterium isolated from fresh water Free

Abstract

A novel Gram-negative bacterium strain, DRW22-8, was isolated from fresh water taken at a depth of 22 m at Daechung Reservoir, Republic of Korea. The cells of strain DRW22-8 were aerobic and motile with a single polar flagellum or non-motile (stalked), and formed creamy-white colonies on R2A agar. The phylogenetic analysis based on 16S rRNA gene sequencing indicated that the strain formed a separate lineage within the order , showing similarity values under 91.8 % with its closest phylogenetic neighbours, , and The chemotaxonomic results showed Q-10 as the predominant respiratory ubiquinone, three unidentified glycolipids, an unidentified lipid and phosphatidylglycerol as the major polar lipids, and C, 11-methyl C, C 7 and/or C 6 as the major fatty acids. The DNA G+C content was 64.4 mol%. The combined genotypic and phenotypic data showed that strain DRW22-8 could be distinguished from all genera within the family and represented a novel genus, gen. nov., with the name sp. nov., in the family . The type strain is DRW22-8 (=KCTC 42356=JCM 30469).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Asprobacter , Asprobacter aquaticus , DRW22-8 and prosthecate
© 2017 IUMS
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.002311
2017-11-01
2025-04-25
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/67/11/4443.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.002311&mimeType=html&fmt=ahah

References

  1. Henrici AT, Johnson DE. Studies of Freshwater Bacteria: II. Stalked bacteria, a new order of Schizomycetes. J Bacteriol 1935; 30:61–93[PubMed]
     [Google Scholar]
  2. Abraham WR, Strömpl C, Vancanneyt M, Bennasar A, Swings J et al. Woodsholea maritima gen. nov., sp. nov., a marine bacterium with a low diversity of polar lipids. Int J Syst Evol Microbiol 2004; 54:1227–1234 [View Article][PubMed]
     [Google Scholar]
  3. Lee K, Lee HK, Choi TH, Cho JC. Robiginitomaculum antarcticum gen. nov., sp. nov., a member of the family Hyphomonadaceae, from Antarctic seawater. Int J Syst Evol Microbiol 2007; 57:2595–2599 [View Article][PubMed]
     [Google Scholar]
  4. Alain K, Tindall BJ, Intertaglia L, Catala P, Lebaron P. Hellea balneolensis gen. nov., sp. nov., a prosthecate alphaproteobacterium from the Mediterranean Sea. Int J Syst Evol Microbiol 2008; 58:2511–2519 [View Article][PubMed]
     [Google Scholar]
  5. Lai Q, Yuan J, Wu C, Shao Z. Oceanibaculum indicum gen. nov., sp. nov., isolated from deep seawater of the Indian Ocean. Int J Syst Evol Microbiol 2009; 59:1733–1737 [View Article][PubMed]
     [Google Scholar]
  6. Kang HS, Lee SD. Ponticaulis koreensis gen. nov., sp. nov., a new member of the family Hyphomonadaceae isolated from seawater. Int J Syst Evol Microbiol 2009; 59:2951–2955 [View Article][PubMed]
     [Google Scholar]
  7. Zhang XY, Li GW, Wang CS, Zhang YJ, Xu XW et al. Marinicauda pacifica gen. nov., sp. nov., a prosthecate alphaproteobacterium of the family Hyphomonadaceae isolated from deep seawater. Int J Syst Evol Microbiol 2013; 63:2248–2253 [View Article][PubMed]
     [Google Scholar]
  8. Fukui Y, Abe M, Kobayashi M, Saito H, Oikawa H et al. Algimonas porphyrae gen. nov., sp. nov., a member of the family Hyphomonadaceae, isolated from the red alga Porphyra yezoensis . Int J Syst Evol Microbiol 2013; 63:314–320 [View Article][PubMed]
     [Google Scholar]
  9. Chen MH, Sheu SY, Chen CA, Wang JT, Chen WM. Oceanicaulis stylophorae sp. nov., isolated from the reef-building coral Stylophora pistillata . Int J Syst Evol Microbiol 2012; 62:2241–2246 [View Article][PubMed]
     [Google Scholar]
  10. Schlesner H, Bartels C, Sittig M, Dorsch M, Stackebrandt E. Taxonomic and phylogenetic studies on a new taxon of budding, hyphal Proteobacteria, Hirschia baltica gen. nov., sp. nov. Int J Syst Bacteriol 1990; 40:443–451 [View Article][PubMed]
     [Google Scholar]
  11. Weiner RM, Melick M, O'Neill K, Quintero E. Hyphomonas adhaerens sp. nov., Hyphomonas johnsonii sp. nov. and Hyphomonas rosenbergii sp. nov., marine budding and prosthecate bacteria. Int J Syst Evol Microbiol 2000; 50:459–469 [View Article][PubMed]
     [Google Scholar]
  12. Abraham WR, Lünsdorf H, Vancanneyt M, Smit J. Cauliform bacteria lacking phospholipids from an abyssal hydrothermal vent: proposal of Glycocaulis abyssi gen. nov., sp. nov., belonging to the family Hyphomonadaceae . Int J Syst Evol Microbiol 2013; 63:2207–2215 [View Article][PubMed]
     [Google Scholar]
  13. Jung JY, Kim JM, Jin HM, Kim SY, Park W et al. Litorimonas taeanensis gen. nov., sp. nov., isolated from a sandy beach. Int J Syst Evol Microbiol 2011; 61:1534–1538 [View Article][PubMed]
     [Google Scholar]
  14. Weiner RM, Devine RA, Powell DM, Dagasan L, Moore RL. Hyphomonas oceanitis sp. nov., Hyphomonas hirschiana sp. nov., and Hyphomonas jannaschiana sp. nov. Int J Syst Bacteriol 1985; 35:237–243 [View Article]
     [Google Scholar]
  15. Strömpl C, Hold GL, Lünsdorf H, Graham J, Gallacher S et al. Oceanicaulis alexandrii gen. nov., sp. nov., a novel stalked bacterium isolated from a culture of the dinoflagellate Alexandrium tamarense (Lebour) Balech. Int J Syst Evol Microbiol 2003; 53:1901–1906 [View Article][PubMed]
     [Google Scholar]
  16. Jin L, Lee CS, Ahn CY, Lee HG, Lee S et al. Abundant iron and sulfur oxidizers in the stratified sediment of a eutrophic freshwater reservoir with annual cyanobacterial blooms. Sci Rep 2017; 7:43814 [View Article][PubMed]
     [Google Scholar]
  17. Bates RG, Bower VE. Alkaline solutions for pH control. Anal Chem 1956; 28:1322–1324 [View Article]
     [Google Scholar]
  18. Gomori G. Preparation of buffers for use in enzyme studies. Methods Enzymol 1955; 1:138–146 [Crossref]
     [Google Scholar]
  19. Tarrand JJ, Gröschel DH. Rapid, modified oxidase test for oxidase-variable bacterial isolates. J Clin Microbiol 1982; 16:772–774[PubMed]
     [Google Scholar]
  20. Tindall BJ. A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. Syst Appl Microbiol 1990; 13:128–130 [View Article]
     [Google Scholar]
  21. Tindall BJ. Lipid composition of Halobacterium lacusprofundi . FEMS Microbiol Lett 1990; 66:199–202 [View Article]
     [Google Scholar]
  22. Tamaoka J, Komagata K. Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 1984; 25:125–128 [View Article]
     [Google Scholar]
  23. Lane DJ. 16S/23S rRNA sequencing. In Stackebrandt E E, Goodfellow M. (editors) Nucleic acid Techniques in Bacterial Systematics Chichester: Wiley; 1991 pp. 115–175
     [Google Scholar]
  24. Yoon SH, Ha SM, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017; 67:1613–1617 [View Article][PubMed]
     [Google Scholar]
  25. Hall TA. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucl Acids Symp Ser 1999; 41:95–98
     [Google Scholar]
  26. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 1997; 25:4876–4882 [View Article][PubMed]
     [Google Scholar]
  27. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980; 16:111–120 [View Article][PubMed]
     [Google Scholar]
  28. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4:406–425[PubMed]
     [Google Scholar]
  29. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981; 17:368–376 [View Article][PubMed]
     [Google Scholar]
  30. Fitch WM. Toward defining the course of evolution: minimum change for a specific tree topology. SystZool 1971; 20:406–416
     [Google Scholar]
  31. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013; 30:2725–2729 [View Article][PubMed]
     [Google Scholar]
  32. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985; 39:783–791 [View Article][PubMed]
     [Google Scholar]
  33. Kang HS, Lee SD. Hirschia maritima sp. nov., isolated from seawater. Int J Syst Evol Microbiol 2009; 59:2264–2268 [View Article][PubMed]
     [Google Scholar]
  34. Park S, Yoon JH. Hirschia litorea sp. nov., isolated from seashore sediment, and emended description of the genus Hirschia . Int J Syst Evol Microbiol 2013; 63:1684–1689 [View Article][PubMed]
     [Google Scholar]
  35. Lee SH, Shim JK, Kim JM, Choi HK, Jeon CO. Henriciella litoralis sp. nov., isolated from a tidal flat, transfer of Maribaculum marinum Lai et al. 2009 to the genus Henriciella as Henriciella aquimarina nom. nov. and emended description of the genus Henriciella . Int J Syst Evol Microbiol 2011; 61:722–727 [View Article][PubMed]
     [Google Scholar]
  36. Moore RL, Weiner RM, Gebers R. Genus Hyphomonas Pongratz 1957 nom. rev. emend., Hyphomonas polymorpha Pongratz 1957 nom. rev. emend., and Hyphomonas neptunium (Leifson 1964) comb. nov. emend. (Hyphomicrobium neptunium). Int J Syst Bacteriol 1984; 34:71–73 [View Article]
     [Google Scholar]
  37. Abraham WR, Strömpl C, Meyer H, Lindholst S, Moore ER et al. Phylogeny and polyphasic taxonomy of Caulobacter species. Proposal of Maricaulis gen. nov. with Maricaulis maris (Poindexter) comb. nov. as the type species, and emended description of the genera Brevundimonas and Caulobacter . Int J Syst Bacteriol 1999; 49:1053–1073 [View Article][PubMed]
     [Google Scholar]
/content/journal/ijsem/10.1099/ijsem.0.002311
Loading
Asprobacter aquaticus gen. nov., sp. nov., a prosthecate alphaproteobacterium isolated from fresh water
Int J Syst Evol Microbiol 67, 4443 (2017); https://doi.org/10.1099/ijsem.0.002311
/content/journal/ijsem/10.1099/ijsem.0.002311
/content/journal/ijsem/10.1099/ijsem.0.002311
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error