RT Journal Article SR Electronic(1) A1 Siddiqi, Muhammad Zubair A1 Choi, Gyu-Min A1 Kim, Sang Yong A1 Choi, Kang Duk A1 Im, Wan-TaekYR 2017 T1 Sphingomonas agri sp. nov., a bacterium isolated from soil JF International Journal of Systematic and Evolutionary Microbiology, VO 67 IS 11 SP 4429 OP 4434 DO https://doi.org/10.1099/ijsem.0.002306 PB Microbiology Society, SN 1466-5034, AB A Gram-reaction-negative, aerobic, non-motile, white (translucent) and rod-shaped bacterium (designated HKS-06T) isolated from soil was characterized by a polyphasic approach to clarify its taxonomic position. Strain HKS-06T was observed to grow optimally at 30 °C and at pH 6.5–7.0 on R2A agar medium. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain HKS-06T belongs to the genus Sphingomonas and is most closely related to Sphingomonas lutea JS5T (97.4 % similarity). The G+C content of the genomic DNA was 64.1 mol%. Chemotaxonomic data [major quinone (Q-10), major polar lipids (phosphatidylethanolamine, phosphatidylglycerol, sphingoglycolipid, phosphatidylcholine, unknown polar lipid) and major fatty acids (summed feature 8, comprising C18 : 1ω7c/ω6c and/or C18 : 1ω6c, C18 : 0 3-OH and C16 : 0)] supported the affiliation of strain HKS-06T to the genus Sphingomonas . Moreover, the physiological and biochemical results and low level of DNA–DNA relatedness [between strain HKS06T and S . lutea JS5T (20.24±1.2 %)] allowed the phenotypic and genotypic differentiation of strain HKS-06T from recognized species of the genus Sphingomonas . The new isolate therefore represents a novel species, for which the name Sphingomonas agri sp. nov. is proposed. The type strain is HKS-06T (=KACC 18880T=LMG 29563T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002306