RT Journal Article SR Electronic(1) A1 Park, Miri A1 Nam, Gi Gyun A1 Kim, Suhyun A1 Jeon, Hyoung Tae A1 Joung, Yochan A1 Cho, Jang-CheonYR 2017 T1 Flavobacterium chuncheonense sp. nov. and Flavobacterium luteum sp. nov., isolated from a freshwater lake JF International Journal of Systematic and Evolutionary Microbiology, VO 67 IS 11 SP 4409 OP 4415 DO https://doi.org/10.1099/ijsem.0.002304 PB Microbiology Society, SN 1466-5034, AB Two Gram-staining-negative, orange coloured, non-motile, rod-shaped bacterial strains, designated strains IMCC26013T and IMCC26026T, were isolated from a freshwater sample collected from Lake Soyang in Korea. The 16S rRNA gene-based phylogenetic analyses showed that both strains belonged to the genus Flavobacterium and that strains IMCC26013T and IMCC26026T were most closely related to Flavobacterium psychrophilum (96.5 %) and Flavobacterium myungsuense (97.7 %), respectively. DNA G+C contents of strains IMCC26013T and IMCC26026T were 37.8 and 33.7 mol%, respectively. DNA–DNA relatedness between strain IMCC26026T and F. myungsuense HMD1033T was 56.4 %, showing a novel species status of strain IMCC26026T. Major fatty acid constituents (>10 %) of strain IMCC26013T were iso-C15 : 1 G, C15 : 1  ω6c, C17 : 1  ω6c and summed feature 3 (C16 : 1  ω6c and/or C16 : 1  ω7c) and those of strain IMCC26026T were iso-C15 : 0, anteiso-C15 : 0 and summed feature 3. Respiratory quinone detected in the two strains was MK-6. Both strains contained phosphatidylethanolamine as a major polar lipid. On the basis of these results, strains IMCC26013T and IMCC26026T were considered to represent novel species in the genus Flavobacterium , for which the names Flavobacterium chuncheonense (type strain IMCC26013T=KCTC 52573T=NBRC 112526T), and Flavobacterium luteum (type strain IMCC26026T=KCTC 52572T=NBRC 112527T) are proposed, respectively., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002304