1887

Abstract

Two Gram-staining-negative, orange coloured, non-motile, rod-shaped bacterial strains, designated strains IMCC26013 and IMCC26026, were isolated from a freshwater sample collected from Lake Soyang in Korea. The 16S rRNA gene-based phylogenetic analyses showed that both strains belonged to the genus and that strains IMCC26013 and IMCC26026 were most closely related to (96.5 %) and (97.7 %), respectively. DNA G+C contents of strains IMCC26013 and IMCC26026 were 37.8 and 33.7 mol%, respectively. DNA–DNA relatedness between strain IMCC26026 and HMD1033 was 56.4 %, showing a novel species status of strain IMCC26026. Major fatty acid constituents (>10 %) of strain IMCC26013 were iso-C G, C 6, C 6 and summed feature 3 (C 6 and/or C 7) and those of strain IMCC26026 were iso-C, anteiso-C and summed feature 3. Respiratory quinone detected in the two strains was MK-6. Both strains contained phosphatidylethanolamine as a major polar lipid. On the basis of these results, strains IMCC26013 and IMCC26026 were considered to represent novel species in the genus , for which the names (type strain IMCC26013=KCTC 52573=NBRC 112526), and (type strain IMCC26026=KCTC 52572=NBRC 112527) are proposed, respectively.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.002304
2017-11-01
2024-10-06
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/67/11/4409.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.002304&mimeType=html&fmt=ahah

References

  1. Bergey DH, Harrison FC, Breed RS, Hammer BW, Huntoon FM et al. Genus II. Flavobacterium gen. nov. In Bergey’s Manual of Determinative Bacteriology, 1st ed. Baltimore, MD: Williams and Wilkins; 1923 pp. 97–117
    [Google Scholar]
  2. Bernardet J-F, Segers P, Vancanneyt M, Berthe F, Kersters K et al. Cutting a Gordian knot: emended classification and description of the genus Flavobacterium, emended description of the family Flavobacteriaceae, and proposal of Flavobacterium hydatis nom. nov. (Basonym, Cytophaga aquatilis Strohl and Tait 1978). Int J Syst Bacteriol 1996; 46:128–148 [Crossref]
    [Google Scholar]
  3. Bernardet JF, Bowman JP. Genus I. Flavobacterium . In Krieg NR, Staley JT, Brown DR, Hedlund BP, Paster BJ et al. (editors) Bergey’s Manual of Systematic Bacteriology, 2nd ed. vol. 4 New York: Springer; 2010 pp. 112–154
    [Google Scholar]
  4. Kang JY, Chun J, Jahng KY. Flavobacterium aciduliphilum sp. nov., isolated from freshwater, and emended description of the genus Flavobacterium . Int J Syst Evol Microbiol 2013; 63:1633–1638 [View Article][PubMed]
    [Google Scholar]
  5. Joung Y, Kim H, Joh K. Flavobacterium jumunjinense sp. nov., isolated from a lagoon, and emended descriptions of Flavobacterium cheniae, Flavobacterium dongtanense and Flavobacterium gelidilacus . Int J Syst Evol Microbiol 2013; 63:3937–3943 [View Article][PubMed]
    [Google Scholar]
  6. Kuo I, Saw J, Kapan DD, Christensen S, Kaneshiro KY et al. Flavobacterium akiainvivens sp. nov., from decaying wood of Wikstroemia oahuensis, Hawai'i, and emended description of the genus Flavobacterium . Int J Syst Evol Microbiol 2013; 63:3280–3286 [View Article][PubMed]
    [Google Scholar]
  7. Feng Q, Gao Y, Nogi Y, Tan X, Han L et al. Flavobacterium maotaiense sp. nov., isolated from freshwater. Int J Syst Evol Microbiol 2015; 65:171–176 [View Article][PubMed]
    [Google Scholar]
  8. Kim H, Kang H, Joung Y, Joh K. Flavobacterium gyeonganense sp. nov., isolated from freshwater, and emended descriptions of Flavobacterium chungangense, Flavobacterium aquidurense, Flavobacterium tructae and Flavobacterium granuli . Int J Syst Evol Microbiol 2014; 64:4173–4178 [View Article][PubMed]
    [Google Scholar]
  9. Lee S, Weon HY, Han K, Ahn TY. Flavobacterium dankookense sp. nov., isolated from a freshwater reservoir, and emended descriptions of Flavobacterium cheonanense, F. chungnamense, F. koreense and F. aquatile . Int J Syst Evol Microbiol 2012; 62:2378–2382 [View Article][PubMed]
    [Google Scholar]
  10. Nupur Bhumika V, Srinivas TN, Kumar PA. Flavobacterium nitratireducens sp. nov., an amylolytic bacterium of the family Flavobacteriaceae isolated from coastal surface seawater. Int J Syst Evol Microbiol 2013; 63:2490–2496 [View Article][PubMed]
    [Google Scholar]
  11. Yoon JH, Park S, Kang SJ, Oh SJ, Myung SC et al. Flavobacterium ponti sp. nov., isolated from seawater. Int J Syst Evol Microbiol 2011; 61:81–85 [View Article][PubMed]
    [Google Scholar]
  12. Dong K, Chen F, Du Y, Wang G. Flavobacterium enshiense sp. nov., isolated from soil, and emended descriptions of the genus Flavobacterium and Flavobacterium cauense, Flavobacterium saliperosum and Flavobacterium suncheonense . Int J Syst Evol Microbiol 2013; 63:886–892 [View Article][PubMed]
    [Google Scholar]
  13. Singh H, Du J, Won K, Yang JE, Akter S et al. Flavobacterium vireti sp. nov., isolated from soil. Antonie van Leeuwenhoek 2015; 107:1421–1428 [View Article][PubMed]
    [Google Scholar]
  14. Yoon JH, Kang SJ, Oh TK, T-k O. Flavobacterium soli sp. nov., isolated from soil. Int J Syst Evol Microbiol 2006; 56:997–1000 [View Article][PubMed]
    [Google Scholar]
  15. van Trappen S, Vandecandelaere I, Mergaert J, Swings J. Flavobacterium fryxellicola sp. nov. and Flavobacterium psychrolimnae sp. nov., novel psychrophilic bacteria isolated from microbial mats in Antarctic lakes. Int J Syst Evol Microbiol 2005; 55:769–772 [View Article][PubMed]
    [Google Scholar]
  16. Yi H, Oh HM, Lee JH, Kim SJ, Chun J. Flavobacterium antarcticum sp. nov., a novel psychrotolerant bacterium isolated from the Antarctic. Int J Syst Evol Microbiol 2005; 55:637–641 [View Article][PubMed]
    [Google Scholar]
  17. Xu M, Xin Y, Tian J, Dong K, Yu Y et al. Flavobacterium sinopsychrotolerans sp. nov., isolated from a glacier. Int J Syst Evol Microbiol 2011; 61:20–24 [View Article][PubMed]
    [Google Scholar]
  18. Kämpfer P, Lodders N, Martin K, Avendaño-Herrera R. Flavobacterium chilense sp. nov. and Flavobacterium araucananum sp. nov., isolated from farmed salmonid fish. Int J Syst Evol Microbiol 2012; 62:1402–1408 [View Article][PubMed]
    [Google Scholar]
  19. Fujii D, Nagai F, Watanabe Y, Shirasawa Y. Flavobacterium longum sp. nov. and Flavobacterium urocaniciphilum sp. nov., isolated from a wastewater treatment plant, and emended descriptions of Flavobacterium caeni and Flavobacterium terrigena . Int J Syst Evol Microbiol 2014; 64:1488–1494 [View Article][PubMed]
    [Google Scholar]
  20. Horn MA, Ihssen J, Matthies C, Schramm A, Acker G et al. Dechloromonas denitrificans sp. nov., Flavobacterium denitrificans sp. nov., Paenibacillus anaericanus sp. nov. and Paenibacillus terrae strain MH72, N2O-producing bacteria isolated from the gut of the earthworm Aporrectodea caliginosa . Int J Syst Evol Microbiol 2005; 55:1255–1265 [View Article][PubMed]
    [Google Scholar]
  21. Cho JC, Giovannoni SJ. Cultivation and growth characteristics of a diverse group of oligotrophic marine gammaproteobacteria. Appl Environ Microbiol 2004; 70:432–440[PubMed] [Crossref]
    [Google Scholar]
  22. Connon SA, Giovannoni SJ. High-throughput methods for culturing microorganisms in very-low-nutrient media yield diverse new marine isolates. Appl Environ Microbiol 2002; 68:3878–3885[PubMed] [Crossref]
    [Google Scholar]
  23. Davis HC, Guillard RRL. Relative value of ten genera of micro-organisms as food for oyster and clam larvae. USFWS Fish Bull 1958; 58:293–304
    [Google Scholar]
  24. Yoon SH, Ha SM, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017; 67:1613–1617 [View Article][PubMed]
    [Google Scholar]
  25. Ludwig W, Strunk O, Westram R, Richter L, Meier H et al. ARB: a software environment for sequence data. Nucleic Acids Res 2004; 32:1363–1371 [View Article][PubMed]
    [Google Scholar]
  26. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4:406–425[PubMed]
    [Google Scholar]
  27. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981; 17:368–376[PubMed] [Crossref]
    [Google Scholar]
  28. Fitch WM. Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 1971; 20:406–416 [Crossref]
    [Google Scholar]
  29. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013; 30:2725–2729 [View Article][PubMed]
    [Google Scholar]
  30. Wayne LG, Brenner DJ, Colwell RR, Grimont PA, Kandler O et al. International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 1987; 37:463–464 [Crossref]
    [Google Scholar]
  31. Gonzalez JM, Saiz-Jimenez C. A fluorimetric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperature. Environ Microbiol 2002; 4:770–773[PubMed] [Crossref]
    [Google Scholar]
  32. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101. Newark, DE: MIDI; 1990
    [Google Scholar]
  33. Minnikin D, O'Donnell A, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984; 2:233–241 [Crossref]
    [Google Scholar]
  34. Collins MD, Shah HN, Minnikin DE. A note on the separation of natural mixtures of bacterial menaquinones using reverse phase thin-layer chromatography. J Appl Bacteriol 1980; 48:277–282[PubMed] [Crossref]
    [Google Scholar]
  35. Ali Z, Cousin S, Frühling A, Brambilla E, Schumann P et al. Flavobacterium rivuli sp. nov., Flavobacterium subsaxonicum sp. nov., Flavobacterium swingsii sp. nov. and Flavobacterium reichenbachii sp. nov., isolated from a hard water rivulet. Int J Syst Evol Microbiol 2009; 59:2610–2617 [View Article][PubMed]
    [Google Scholar]
  36. Chen WM, Chen JC, Sheu SY. Flavobacterium oryzae sp. nov., isolated from a flooded rice field, and emended descriptions of Flavobacterium flevense, Flavobacterium yonginense and Flavobacterium myungsuense . Int J Syst Evol Microbiol 2014; 64:3701–3708 [View Article][PubMed]
    [Google Scholar]
  37. Joung Y, Kim H, Ahn TS, Joh K. Flavobacterium yonginense sp. nov. and Flavobacterium myungsuense sp. nov., isolated from a mesotrophic artificial lake. Int J Syst Evol Microbiol 2012; 62:806–810 [View Article][PubMed]
    [Google Scholar]
  38. Montero-Calasanz MC, Göker M, Rohde M, Spröer C, Schumann P et al. Chryseobacterium hispalense sp. nov., a plant-growth-promoting bacterium isolated from a rainwater pond in an olive plant nursery, and emended descriptions of Chryseobacterium defluvii, Chryseobacterium indologenes, Chryseobacterium wanjuense and Chryseobacterium gregarium . Int J Syst Evol Microbiol 2013; 63:4386–4395 [View Article][PubMed]
    [Google Scholar]
/content/journal/ijsem/10.1099/ijsem.0.002304
Loading
/content/journal/ijsem/10.1099/ijsem.0.002304
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error