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Abstract

A strain of lactic acid bacteria, designated 159469, isolated from a facial abscess in a sugar glider, was characterized genetically and phenotypically. Cells of the strain were Gram-stain-positive, coccoid and catalase-negative. Morphological, physiological and phylogenetic data indicated that the isolate belongs to the genus Lactococcus . Strain 159469 was closely related to Lactococcus garvieae ATCC 43921, showing 95.86 and 98.08 % sequence similarity in 16S rRNA gene and rpoB gene sequences, respectively. Furthermore, a pairwise average nucleotide identity blast (ANIb) value of 93.54 % and in silico DNA–DNA hybridization value of 50.7  % were determined for the genome of strain 159469, when compared with the genome of the type strain of Lactococcus garvieae . Based on the data presented here, the isolate represents a novel species of the genus Lactococcus , for which the name Lactococcus petauri sp. nov. is proposed. The type strain is 159469 (=LMG 30040=DSM 104842).

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2017-09-25
2019-12-08
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References

  1. Lennox AM, Miwa Y. Anatomy and disorders of the oral cavity of miscellaneous exotic companion mammals. Vet Clin North Am Exot Anim Pract 2016; 19: 929– 945 [CrossRef] [PubMed]
    [Google Scholar]
  2. Ness R, Johnson-Delaney C. Sugar gliders. In: Ferrets Rabbits Rodents Clinical Medicine and Surgery, 2nd ed. St Louis, MO, USA: Elsevier Saunders; 2012; pp. 393– 410 [Crossref]
    [Google Scholar]
  3. Johnson R, Hemsley S. Gliders and possums. In Vogelnest L, Woods R. (editors) Medicine of Australian Mammals Australia: CSIRO Publishing; 2008; pp. 395– .438
    [Google Scholar]
  4. Collins MD, Farrow JA, Phillips BA, Kandler O. Streptococcus garvieae sp. nov. and Streptococcus plantarum sp. nov. J Gen Microbiol 1983; 129: 3427– 3431 [CrossRef] [PubMed]
    [Google Scholar]
  5. Wang CY, Shie HS, Chen SC, Huang JP, Hsieh IC et al. Lactococcus garvieae infections in humans: possible association with aquaculture outbreaks. Int J Clin Pract 2007; 61: 68– 73 [CrossRef] [PubMed]
    [Google Scholar]
  6. Navas ME, Hall G, El Bejjani D. A case of endocarditis caused by Lactococcus garvieae and suggested methods for identification. J Clin Microbiol 2013; 51: 1990– 1992 [CrossRef] [PubMed]
    [Google Scholar]
  7. Ferrario C, Ricci G, Milani C, Lugli GA, Ventura M et al. Lactococcus garvieae: where is it from? A first approach to explore the evolutionary history of this emerging pathogen. PLoS One 2013; 8: e84796 [CrossRef] [PubMed]
    [Google Scholar]
  8. Meyburgh CM, Bragg RR, Boucher CE. Lactococcus garvieae: an emerging bacterial pathogen of fish. Dis Aquat Organ 2017; 123: 67– 79 [CrossRef] [PubMed]
    [Google Scholar]
  9. Lagesen K, Hallin P, Rødland EA, Staerfeldt HH, Rognes T et al. RNAmmer: consistent and rapid annotation of ribosomal RNA genes. Nucleic Acids Res 2007; 35: 3100– 3108 [CrossRef] [PubMed]
    [Google Scholar]
  10. Wright ES, Yilmaz LS, Noguera DR. DECIPHER, a search-based approach to chimera identification for 16S rRNA sequences. Appl Environ Microbiol 2012; 78: 717– 725 [CrossRef] [PubMed]
    [Google Scholar]
  11. Stamatakis A. Using RAxML to Infer Phylogenies. Curr Protoc Bioinforma 2015; 51: 6– 14 [CrossRef] [PubMed]
    [Google Scholar]
  12. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013; 30: 2725– 2729 [CrossRef] [PubMed]
    [Google Scholar]
  13. Kim M, Oh HS, Park SC, Chun J. Towards a taxonomic coherence between average nucleotide identity and 16S rRNA gene sequence similarity for species demarcation of prokaryotes. Int J Syst Evol Microbiol 2014; 64: 346– 351 [CrossRef] [PubMed]
    [Google Scholar]
  14. Bankevich A, Nurk S, Antipov D, Gurevich AA, Dvorkin M et al. SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing. J Comput Biol 2012; 19: 455– 477 [CrossRef] [PubMed]
    [Google Scholar]
  15. Li H, Handsaker B, Wysoker A, Fennell T, Ruan J et al. The sequence alignment/map format and SAMtools. Bioinformatics 2009; 25: 2078– 2079 [CrossRef] [PubMed]
    [Google Scholar]
  16. Gurevich A, Saveliev V, Vyahhi N, Tesler G. QUAST: quality assessment tool for genome assemblies. Bioinformatics 2013; 29: 1072– 1075 [CrossRef] [PubMed]
    [Google Scholar]
  17. Gardner SN, Hall BG. When whole-genome alignments just won't work: kSNP v2 software for alignment-free SNP discovery and phylogenetics of hundreds of microbial genomes. PLoS One 2013; 8: e81760 [CrossRef] [PubMed]
    [Google Scholar]
  18. Core Team R. R: A Language and Environment for Statistical Computing Vienna, Austria: R Foundation for Statistical Computing; 2014
    [Google Scholar]
  19. Richter M, Rosselló-Móra R. Shifting the genomic gold standard for the prokaryotic species definition. Proc Natl Acad Sci USA 2009; 106: 19126– 19131 [CrossRef] [PubMed]
    [Google Scholar]
  20. Meier-Kolthoff JP, Göker M, Spröer C, Klenk HP. When should a DDH experiment be mandatory in microbial taxonomy?. Arch Microbiol 2013; 195: 413– 418 [CrossRef] [PubMed]
    [Google Scholar]
  21. Meier-Kolthoff JP, Klenk HP, Göker M. Taxonomic use of DNA G+C content and DNA-DNA hybridization in the genomic age. Int J Syst Evol Microbiol 2014; 64: 352– 356 [CrossRef] [PubMed]
    [Google Scholar]
  22. Chen YS, Otoguro M, Lin YH, Pan SF, Ji SH et al. Lactococcus formosensis sp. nov., a lactic acid bacterium isolated from yan-tsai-shin (fermented broccoli stems). Int J Syst Evol Microbiol 2014; 64: 146– 151 [CrossRef] [PubMed]
    [Google Scholar]
  23. Cai Y, Yang J, Pang H, Kitahara M. Lactococcus fujiensis sp. nov., a lactic acid bacterium isolated from vegetable matter. Int J Syst Evol Microbiol 2011; 61: 1590– 1594 [CrossRef] [PubMed]
    [Google Scholar]
  24. Cho SL, Nam SW, Yoon JH, Lee JS, Sukhoom A et al. Lactococcus chungangensis sp. nov., a lactic acid bacterium isolated from activated sludge foam. Int J Syst Evol Microbiol 2008; 58: 1844– 1849 [CrossRef] [PubMed]
    [Google Scholar]
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