1887

Abstract

A sulfur-oxidizing bacterium, designated strain KBB12, was isolated from swinery waste collected in Jeju, Republic of Korea. The cells were Gram-stain-negative, flagellated and rod-shaped. Growth occurred at 15–45 °C (optimum, 30–37 °C), at pH 6–9 (optimum, pH 7.0) and in the presence of 0–1 % (w/v) NaCl. The major cellular fatty acids were summed feature 3 (iso-C 2-OH and/or C ω7, C and Cω7. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phospholipid and an unidentified lipid. The major isoprenoid quinone was ubiquinone-8 (Q-8) and the DNA G+C content of the genomic DNA was 69.6 mol%. Phylogenetic analyses, based on 16S rRNA gene sequences, showed that the novel isolate belongs to the genus and was most closely related to CY1 (97.2 % similarity). The DNA–DNA relatedness values between strain KBB12 and DSM26005 was 43.4 2.7 %. On the basis of phylogenetic and phenotypic evidence, it is proposed that strain KBB12 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is KBB12 (=KCTC 32230=JCM 18740).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.002294
2018-01-01
2024-04-20
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/68/1/9.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.002294&mimeType=html&fmt=ahah

References

  1. Wang H, Li J, Hu A, Qin D, Xu H et al. Melaminivora alkalimesophila gen. nov., sp. nov., a melamine-degrading betaproteobacterium isolated from a melamine-producing factory. Int J Syst Evol Microbiol 2014; 64:1938–1944 [View Article][PubMed]
    [Google Scholar]
  2. Liao B, Ji G, Cheng L. Profling of microbial communities in a bioreactor for treating hydrocarbon-sulfde-containing wastewater. J Environ Sci 2008; 20:897–899 [View Article]
    [Google Scholar]
  3. Luo JF, Lin WT, Guo Y. Functional genes based analysis of sulfur-oxidizing bacteria community in sulfide removing bioreactor. Appl Microbiol Biotechnol 2011; 90:769–778 [View Article][PubMed]
    [Google Scholar]
  4. Petri R, Podgorsek L, Imhoff JF. Phylogeny and distribution of the soxB gene among thiosulfate-oxidizing bacteria. FEMS Microbiol Lett 2001; 197:171–178 [View Article][PubMed]
    [Google Scholar]
  5. Sorokin DY, van den Bosch PL, Abbas B, Janssen AJ, Muyzer G. Microbiological analysis of the population of extremely haloalkaliphilic sulfur-oxidizing bacteria dominating in lab-scale sulfide-removing bioreactors. Appl Microbiol Biotechnol 2008; 80:965–975 [View Article][PubMed]
    [Google Scholar]
  6. Anandham R, Indiragandhi P, Madhaiyan M, Ryu KY, Jee HJ et al. Chemolithoautotrophic oxidation of thiosulfate and phylogenetic distribution of sulfur oxidation gene (soxB) in rhizobacteria isolated from crop plants. Res Microbiol 2008; 159:579–589 [View Article][PubMed]
    [Google Scholar]
  7. Meyer B, Imhoff JF, Kuever J. Molecular analysis of the distribution and phylogeny of the soxB gene among sulfur-oxidizing bacteria - evolution of the Sox sulfur oxidation enzyme system. Environ Microbiol 2007; 9:2957–2977 [View Article][PubMed]
    [Google Scholar]
  8. Bushnell LD, Haas HF. The utilization of certain hydrocarbons by microorganisms. J Bacteriol 1941; 41:653–673[PubMed]
    [Google Scholar]
  9. Weisburg WG, Barns SM, Pelletier DA, Lane DJ. 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 1991; 173:697–703 [View Article][PubMed]
    [Google Scholar]
  10. Kim OS, Cho YJ, Lee K, Yoon SH, Kim M et al. Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 2012; 62:716–721 [View Article][PubMed]
    [Google Scholar]
  11. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 1997; 25:4876–4882 [View Article][PubMed]
    [Google Scholar]
  12. Hall TA. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 1999; 41:95–98
    [Google Scholar]
  13. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4:406–425[PubMed]
    [Google Scholar]
  14. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981; 17:368–376 [View Article][PubMed]
    [Google Scholar]
  15. Kluge AG, Farris JS. Quantitative phyletics and the evolution of anurans. Syst Zool 1969; 18:1–32 [View Article]
    [Google Scholar]
  16. Tamura K, Peterson D, Peterson N, Stecher G, Nei M et al. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 2011; 28:2731–2739 [View Article][PubMed]
    [Google Scholar]
  17. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985; 39:783–791 [View Article][PubMed]
    [Google Scholar]
  18. Ezaki T, Hashimoto Y, Yabuuchi E. Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 1989; 39:224–229 [View Article]
    [Google Scholar]
  19. Cowan ST, Steel KJ. Manual for the Identification of Medical Bacteria London: Cambridge University Press; 1965
    [Google Scholar]
  20. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.; 1990
    [Google Scholar]
  21. Minnikin DE, O'Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984; 2:233–241 [View Article]
    [Google Scholar]
  22. Komagata K, Suzuki K. Lipids and cell-wall analysis in bacterial systematics. Methods Microbiol 1987; 19:161–207 [Crossref]
    [Google Scholar]
  23. Tamaoka J, Komagata K. Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 1984; 25:125–128 [View Article]
    [Google Scholar]
  24. Wayne LG, Brenner DJ, Colwell RR, Grimont PAD, Kandler O et al. International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 1987; 37:463–464 [Crossref]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.002294
Loading
/content/journal/ijsem/10.1099/ijsem.0.002294
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error