1887

Abstract

A novel strain, DKC-1, was isolated from reclaimed grassland soil and was characterized taxonomically by a polyphasic approach. Strain DKC-1 was a Gram-staining-negative, light-yellow-coloured and rod shaped bacterium, motile with polar flagellum. It was able to grow at 20–37 °C, at pH 4.5–9.0 and with 0–3 % (w/v) NaCl concentration. Based on the 16S rRNA gene sequence analysis, strain DKC-1 formed a clade within the members of the genus and showed highest sequence similarities to XD53 (98.36 %), sjH1 (97.92 %), THG-DD7 (97.74 %), THG-B117 (97.65 %) and GP18-1 (97.40 %). The only respiratory quinone was ubiquinone-8. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and phosphatidyl--methylethanolamine. The predominant fatty acids of strain DKC-1 were iso-C, iso-C, summed feature 9 (iso-Cω9 and/or C 10-methyl), iso-C, iso-C 3-OH and iso-C. The genomic DNA G+C content of this novel strain was 63.1 mol%. The DNA–DNA relatedness between strain DKC-1 and its reference strains ( XD53, sjH1, THG-DD7, THG-B117 and GP18-1) was 52.3, 44.7, 38.7, 49.0 and 32.7 %, respectively, which falls below the threshold value of 70 % for the strain to be considered as novel. The morphological, physiological, chemotaxonomic and phylogenetic analyses clearly distinguished this strain from its closest phylogenetic neighbours. Thus, strain DKC-1 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is DKC-1 (=KEMB 9005-571=KACC 19176=JCM 31925).

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2017-10-01
2020-01-17
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