sp. nov., isolated from ice core Free

Abstract

A Gram-stain-negative, rod-shaped bacterial strain, designed RC4-10-4, belonging to the genus , was isolated from the East Rongbuk Glacier on the Tibetan Plateau. Strain RC4-10-4 grew optimally at pH 7.0, at 25 °C and in the presence of 2 % (w/v) NaCl. Summed feature 3 (C ω6 and/or iso-C 2-OH), summed feature 8 (C ω7 and/or C ω6) and C were the major fatty acids. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, sphingoglycolipid and phosphatidylcholine. Carotenoid was detected in the cells. The DNA G+C content of the novel strain was 66.4 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain RC4-10-4 formed a distinct phylogenetic lineage within the cluster comprising strains. Similarities between the 16S rRNA gene sequences of strain RC4-10-4 and the closely related strains KCTC 42179, KCTC 22330, KCTC 23981 and KCTC 42697 were 98.0, 97.6, 97.5 and 97.2 %. The DNA–DNA hybridization values were 37.6, 15.4, 29.8 and 35.8 %, respectively. Based on the phenotypic and phylogenetic characteristics, strain RC4-10-4 represents a novel species of the genus , for which the name sp. nov. is proposed, with the type strain RC4-10-4 (=CGMCC 1.15507=JCM 31277).

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2017-10-01
2024-03-28
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