1887

Abstract

A Gram-positive, halophilic actinobacterial strain Miq-12 was isolated from Meighan wetland in Iran. Strain Miq-12 was strictly aerobic, catalase positive and oxidase negative. The isolate grew at 12–25 % NaCl, at 30–50 °C and pH 5.5–10.5. The optimum NaCl, temperature and pH for growth were 15–20 %, 40 °C and 7.0–8.0, respectively. The cell wall of strain Miq-12 contained -diaminopimelic acid as diagnostic diamino acid and arabinose as whole-cell sugar. The polar lipid pattern consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine and phosphatidylinositol. It synthesized cellular fatty acids of anteiso and iso-branched types, anteiso-C, iso-C0, iso-C iso-C. The major respiratory quinone was MK-9(H). The G+C content of its genomic DNA was 72.1 mol%. Phylogenetic analysis based on 16S rRNA gene sequence comparison revealed that strain Miq-12 belongs to the family constituted a separate clade, and showed the closest phylogenetic similarity to TRM 46074 (96.99 %) and CCUG 63370 (96.92 %). On the basis of phylogenetic analysis, phenotypic and chemotaxonomic characteristics, a novel genus and species of the family gen. nov., sp. nov., are proposed. The type strain is Miq-12 (=IBRCM 11033=LMG 28390). We also propose that should be transferred to this new genus and be named comb. nov. and comb. nov., respectively. The type strain of comb. nov. is TRM 46074 (=CCTCCAA 2012014=JCM 30185) and the type strain of comb. nov. is CCUG 63370 (=DSM 45606=CECT 8304).

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2017-10-01
2019-12-14
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