1887

Abstract

The genus Thiomicrorhabdus (Tmr) in the Piskirickettsiaceae in the Thiotrichales of the Gammaproteobacteria contains four species of sulfur-oxidising obligate chemolithoautotroph with validly published names, all previously classified as Thiomicrospira (Tms) species. Here we demonstrate that Thiomicrospira hydrogeniphila , a recently published hydrogen-utilising chemolithoautotroph closely related to Thiomicrorhabdus frisia (type species of Thiomicrorhabdus ) should be classified as a member of the genus Thiomicrorhabdus and not Thiomicrospira , as Thiomicrorhabdus hydrogeniphila comb. nov., on the basis of comparative physiology and morphology as well as 16S rRNA (rrs) gene identity of Tms. hydrogeniphila MAS2 being closer to that of Tmr. frisia JB-A2 (99.1 %) than to Tms. pelophila DSM 1534 (90.5 %) or Hydrogenovibrio marinus MH-110 (94.1 %), and on the basis of the topology of 16S rRNA gene maximum likelihood trees, which clearly place Tms. hydrogeniphila within the genus Thiomicrorhabdus . It was also noted that thiosulfate-grown Thiomicrorhabdus spp. can be distinguished from Thiomicrospira spp. or Hydrogenovibrio spp. on the basis of the 3 dominant fatty acids (C16 : 1, C18 : 1 and C16 : 0), and from other Thiomicrorhabdus spp. on the basis of the fourth dominant fatty acid, which varies between the species of this genus – which could provide a useful diagnostic method. We provide an emended description of Thiomicrorhabdus (Boden R, Scott KM, Williams J, Russel S, Antonen K et al. Int J Syst Evol Microbiol 2017;67:1140–1151) to take into account the properties of Thiomicrorhabdus hydrogeniphila comb. nov.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.002279
2017-09-18
2019-10-17
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/67/10/4205.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.002279&mimeType=html&fmt=ahah

References

  1. Boden R, Scott KM, Williams J, Russel S, Antonen K et al. An evaluation of Thiomicrospira, Hydrogenovibrio and Thioalkalimicrobium: reclassification of four species of Thiomicrospira to each Thiomicrorhabdus gen. nov. and Hydrogenovibrio, and reclassification of all four species of Thioalkalimicrobium to Thiomicrospira. Int J Syst Evol Microbiol 2017;67:1140–1151 [CrossRef][PubMed]
    [Google Scholar]
  2. Watsuji TO, Hada E, Miyazaki M, Ichimura M, Takai K. Thiomicrospira hydrogeniphila sp. nov., an aerobic, hydrogen- and sulfur-oxidizing chemolithoautotroph isolated from a seawater tank containing a block of beef tallow. Int J Syst Evol Microbiol 2016;66:3688–3693 [CrossRef][PubMed]
    [Google Scholar]
  3. Brinkhoff T, Muyzer G, Wirsen CO, Kuever J. Thiomicrospira kuenenii sp. nov. and Thiomicrospira frisia sp. nov., two mesophilic obligately chemolithoautotrophic sulfur-oxidizing bacteria isolated from an intertidal mud flat. Int J Syst Bacteriol 1999;49:385–392 [CrossRef][PubMed]
    [Google Scholar]
  4. Brinkhoff T, Muyzer G, Wirsen CO, Kuever J. Thiomicrospira chilensis sp. nov., a mesophilic obligately chemolithoautotrophic sulfuroxidizing bacterium isolated from a Thioploca mat. Int J Syst Bacteriol 1999;49:875–879 [CrossRef][PubMed]
    [Google Scholar]
  5. Knittel K, Kuever J, Meyerdierks A, Meinke R, Amann R et al. Thiomicrospira arctica sp. nov. and Thiomicrospira psychrophila sp. nov., psychrophilic, obligately chemolithoautotrophic, sulfur-oxidizing bacteria isolated from marine Arctic sediments. Int J Syst Evol Microbiol 2005;55:781–786 [CrossRef][PubMed]
    [Google Scholar]
  6. Jolley KA, Bliss CM, Bennett JS, Bratcher HB, Brehony C et al. Ribosomal multilocus sequence typing: universal characterization of bacteria from domain to strain. Microbiology 2012;158:1005–1015 [CrossRef][PubMed]
    [Google Scholar]
  7. Edgar RC. MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res 2004;32:1792–1797 [CrossRef][PubMed]
    [Google Scholar]
  8. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 2016;33:1870–1874 [CrossRef][PubMed]
    [Google Scholar]
  9. Tamura K, Nei M. Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Mol Biol Evol 1993;10:512–526[PubMed]
    [Google Scholar]
  10. Yarza P, Yilmaz P, Pruesse E, Glöckner FO, Ludwig W et al. Uniting the classification of cultured and uncultured bacteria and archaea using 16S rRNA gene sequences. Nat Rev Microbiol 2014;12:635–645 [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.002279
Loading
/content/journal/ijsem/10.1099/ijsem.0.002279
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error