1887

Abstract

The genus () in the in the of the contains four species of sulfur-oxidising obligate chemolithoautotroph with validly published names, all previously classified as () species. Here we demonstrate that , a recently published hydrogen-utilising chemolithoautotroph closely related to (type species of ) should be classified as a member of the genus and not , as comb. nov., on the basis of comparative physiology and morphology as well as 16S rRNA () gene identity of MAS2 being closer to that of JB-A2 (99.1 %) than to DSM 1534 (90.5 %) or MH-110 (94.1 %), and on the basis of the topology of 16S rRNA gene maximum likelihood trees, which clearly place within the genus . It was also noted that thiosulfate-grown spp. can be distinguished from spp. or spp. on the basis of the 3 dominant fatty acids (C, C and C), and from other spp. on the basis of the fourth dominant fatty acid, which varies between the species of this genus – which could provide a useful diagnostic method. We provide an emended description of (Boden R, Scott KM, Williams J, Russel S, Antonen K 2017;67:1140–1151) to take into account the properties of comb. nov.

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2017-10-01
2020-04-01
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References

  1. Boden R, Scott KM, Williams J, Russel S, Antonen K et al. An evaluation of Thiomicrospira, Hydrogenovibrio and Thioalkalimicrobium: reclassification of four species of Thiomicrospira to each Thiomicrorhabdus gen. nov. and Hydrogenovibrio, and reclassification of all four species of Thioalkalimicrobium to Thiomicrospira. Int J Syst Evol Microbiol 2017;67:1140–1151 [CrossRef][PubMed]
    [Google Scholar]
  2. Watsuji TO, Hada E, Miyazaki M, Ichimura M, Takai K. Thiomicrospira hydrogeniphila sp. nov., an aerobic, hydrogen- and sulfur-oxidizing chemolithoautotroph isolated from a seawater tank containing a block of beef tallow. Int J Syst Evol Microbiol 2016;66:3688–3693 [CrossRef][PubMed]
    [Google Scholar]
  3. Brinkhoff T, Muyzer G, Wirsen CO, Kuever J. Thiomicrospira kuenenii sp. nov. and Thiomicrospira frisia sp. nov., two mesophilic obligately chemolithoautotrophic sulfur-oxidizing bacteria isolated from an intertidal mud flat. Int J Syst Bacteriol 1999;49:385–392 [CrossRef][PubMed]
    [Google Scholar]
  4. Brinkhoff T, Muyzer G, Wirsen CO, Kuever J. Thiomicrospira chilensis sp. nov., a mesophilic obligately chemolithoautotrophic sulfuroxidizing bacterium isolated from a Thioploca mat. Int J Syst Bacteriol 1999;49:875–879 [CrossRef][PubMed]
    [Google Scholar]
  5. Knittel K, Kuever J, Meyerdierks A, Meinke R, Amann R et al. Thiomicrospira arctica sp. nov. and Thiomicrospira psychrophila sp. nov., psychrophilic, obligately chemolithoautotrophic, sulfur-oxidizing bacteria isolated from marine Arctic sediments. Int J Syst Evol Microbiol 2005;55:781–786 [CrossRef][PubMed]
    [Google Scholar]
  6. Jolley KA, Bliss CM, Bennett JS, Bratcher HB, Brehony C et al. Ribosomal multilocus sequence typing: universal characterization of bacteria from domain to strain. Microbiology 2012;158:1005–1015 [CrossRef][PubMed]
    [Google Scholar]
  7. Edgar RC. MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res 2004;32:1792–1797 [CrossRef][PubMed]
    [Google Scholar]
  8. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 2016;33:1870–1874 [CrossRef][PubMed]
    [Google Scholar]
  9. Tamura K, Nei M. Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Mol Biol Evol 1993;10:512–526[PubMed]
    [Google Scholar]
  10. Yarza P, Yilmaz P, Pruesse E, Glöckner FO, Ludwig W et al. Uniting the classification of cultured and uncultured bacteria and archaea using 16S rRNA gene sequences. Nat Rev Microbiol 2014;12:635–645 [CrossRef][PubMed]
    [Google Scholar]
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