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Abstract

A novel Gram-stain-negative bacterial strain, designated strain S10, was isolated from soil collected in a rice field in Goyang, South Korea. Cells of strain S10 were strictly aerobic, motile and rod-shaped. Colonies were round, convex, smooth and white. The strain grew optimally at 37 °C, pH 7.0 and 0 % (w/v) NaCl. Phylogenetic analysis of the 16S rRNA gene sequence of strain S10 revealed that the bacterium belongs to the family Comamonadaceae and is related to members of the genus Hydrogenophaga , with Hydrogenophaga caeni EMB71 being its closest relative (97.9 % sequence similarity). The DNA G+C content of strain S10 was 68.2 mol%. Strain S10 contained phosphatidylethanolamine and diphosphatidylglycerol as the major polar lipids. The major fatty acids were C16 : 0 and summed feature 3 (C16 : 1 ω7c and/or iso-C15 : 0 2-OH). The predominant respiratory quinone was ubiquinone Q-8. DNA–DNA hybridization values of strain S10 with Hydrogenophaga caeni KCTC 12613, Hydrogenophaga atypica DSM 15342 and Hydrogenophaga defluvii DSM 15341 were 16.1±4.8, 49.0±3.2 and 21.9±8.8 %, respectively. Based on phylogenetic distinctiveness, DNA–DNA hybridization and specific physiological and biochemical characteristics, strain S10 (=KCTC 52520=JCM 31711) is classified as a novel species of the genus Hydrogenophaga , for which the name Hydrogenophaga soli sp. nov. is proposed.

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2017-09-18
2019-10-19
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