1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 67, Issue 10

sp. nov., isolated from seawater Free

Abstract

A Gram-stain-negative, aerobic, non-motile and coccoid, ovoid or rod-shaped bacterial strain, designated OISW-6, was isolated from seawater near Oido, a South Korean island, and subjected to a polyphasic taxonomic study. Strain OISW-6 grew optimally at 25 °C, at pH 7.0–8.0 and in the presence of 2.0–3.0 % (w/v) NaCl. The phylogenetic trees based on 16S rRNA gene sequences showed that strain OISW-6 fell within the clade comprising the type strains of species. Strain OISW-6 exhibited 16S rRNA gene sequence similarity values of 97.0–98.9 % to , , , , and , and of 94.0–96.3 % to the type strains of the other species. Strain OISW-6 contained Q-10 as the predominant ubiquinone and C as the major fatty acid. The major polar lipids detected in strain OISW-6 were phosphatidylcholine, phosphatidylglycerol and one unidentified aminolipid. The DNA G+C content of strain OISW-6 was 57.3 mol% and its DNA–DNA relatedness values with the type strains of the six phylogenetically closely related species were 8–25 %. Differential phenotypic properties, together with its phylogenetic and genetic distinctiveness, revealed that strain OISW-6 is separated from recognized species of the genus . On the basis of the data presented, strain OISW-6 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is OISW-6 (=KCTC 52837=NBRC 112781).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Loktanella acticola sp. nov , novel species , polyphasic taxonomy and seawater
© 2017 IUMS
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.002274
2017-10-01
2024-04-16
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/67/10/4175.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.002274&mimeType=html&fmt=ahah

References

  1. Van Trappen S, Mergaert J, Swings J. Loktanella salsilacus gen. nov., sp. nov., Loktanella fryxellensis sp. nov. and Loktanella vestfoldensis sp. nov., new members of the Rhodobacter group, isolated from microbial mats in Antarctic lakes. Int J Syst Evol Microbiol 2004; 54:1263–1269 [View Article][PubMed]
     [Google Scholar]
  2. Parte AC. LPSN-list of prokaryotic names with standing in nomenclature. Nucleic Acids Res 2014; 42:D613–D616 [View Article][PubMed]
     [Google Scholar]
  3. Lau SC, Tsoi MM, Li X, Plakhotnikova I, Wu M et al. Loktanella hongkongensis sp. nov., a novel member of the α-Proteobacteria originating from marine biofilms in Hong Kong waters. Int J Syst Evol Microbiol 2004; 54:2281–2284 [View Article][PubMed]
     [Google Scholar]
  4. Ivanova EP, Zhukova NV, Lysenko AM, Gorshkova NM, Sergeev AF et al. Loktanella agnita sp. nov. and Loktanella rosea sp. nov., from the north-west Pacific ocean. Int J Syst Evol Microbiol 2005; 55:2203–2207 [View Article][PubMed]
     [Google Scholar]
  5. Weon HY, Kim BY, Yoo SH, Kim JS, Kwon SW et al. Loktanella koreensis sp. nov., isolated from sea sand in Korea. Int J Syst Evol Microbiol 2006; 56:2199–2202 [View Article][PubMed]
     [Google Scholar]
  6. Yoon JH, Kang SJ, Lee SY, Oh TK. Loktanella maricola sp. nov., isolated from seawater of the East sea in Korea. Int J Syst Evol Microbiol 2007; 57:1799–1802 [View Article][PubMed]
     [Google Scholar]
  7. Yoon JH, Jung YT, Lee JS. Loktanella litorea sp. nov., isolated from seawater. Int J Syst Evol Microbiol 2013; 63:175–180 [View Article][PubMed]
     [Google Scholar]
  8. Hosoya S, Yokota A. Loktanella atrilutea sp. nov., isolated from seawater in Japan. Int J Syst Evol Microbiol 2007; 57:1966–1969 [View Article][PubMed]
     [Google Scholar]
  9. Park S, Jung YT, Yoon JH. Loktanella sediminilitoris sp. nov., isolated from tidal flat sediment. Int J Syst Evol Microbiol 2013; 63:4118–4123 [View Article][PubMed]
     [Google Scholar]
  10. Park S, Lee JS, Lee KC, Yoon JH. Loktanella soesokkakensis sp. nov., isolated from the junction between the North Pacific ocean and a freshwater spring. Antonie van Leeuwenhoek 2013; 104:397–404 [View Article][PubMed]
     [Google Scholar]
  11. Tanaka N, Romanenko LA, Kurilenko VV, Svetashev VI, Kalinovskaya NI et al. Loktanella maritima sp. nov. isolated from shallow marine sediments. Int J Syst Evol Microbiol 2014; 64:2370–2375 [View Article][PubMed]
     [Google Scholar]
  12. Jung YT, Park S, Lee JS, Yoon JH. Loktanella marina sp. nov., isolated from seawater. Int J Syst Evol Microbiol 2016; 66:2528–2533 [View Article]
     [Google Scholar]
  13. Park S, Won SM, Kim H, Park DS, Yoon JH. Aestuariivita boseongensis gen. nov., sp. nov., isolated from a tidal flat sediment. Int J Syst Evol Microbiol 2014; 64:2969–2974 [View Article][PubMed]
     [Google Scholar]
  14. Lányí B. Classical and rapid identification methods for medically important bacteria. Methods Microbiol 1987; 19:1–67
     [Google Scholar]
  15. Barrow GI, Feltham RKA. Cowan and Steel’s Manual for the Identification of Medical Bacteria, 3rd ed. Cambridge: Cambridge University Press; 1993 [Crossref]
     [Google Scholar]
  16. Bruns A, Rohde M, Berthe-Corti L. Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North sea intertidal sediment. Int J Syst Evol Microbiol 2001; 51:1997–2006 [View Article][PubMed]
     [Google Scholar]
  17. Leifson E. Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 1963; 85:1183–1184[PubMed]
     [Google Scholar]
  18. Yoon J-H, Kim H, Kim S-B, Kim H-J, Kim WY et al. Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol 1996; 46:502–505 [View Article]
     [Google Scholar]
  19. Yoon J-H, Lee ST, Kim S-B, Kim WY, Goodfellow M et al. Restriction fragment length polymorphism analysis of PCR-amplified 16S ribosomal DNA for rapid identification of Saccharomonospora strains. Int J Syst Bacteriol 1997; 47:111–114 [View Article]
     [Google Scholar]
  20. Yoon JH, Kim IG, Shin DY, Kang KH, Park YH et al. Microbulbifer salipaludis sp. nov., a moderate halophile isolated from a Korean salt marsh. Int J Syst Evol Microbiol 2003; 53:53–57 [View Article][PubMed]
     [Google Scholar]
  21. Ezaki T, Hashimoto Y, Yabuuchi E. Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 1989; 39:224–229 [View Article]
     [Google Scholar]
  22. Komagata K, Suzuki K. Lipids and cell-wall analysis in bacterial systematics. Methods Microbiol 1987; 19:161–207 [Crossref]
     [Google Scholar]
  23. Moon YG, Seo SH, Lee SD, Heo MS. Loktanella pyoseonensis sp. nov., isolated from beach sand, and emended description of the genus Loktanella . Int J Syst Evol Microbiol 2010; 60:785–789 [View Article][PubMed]
     [Google Scholar]
  24. Lee SD. Loktanella tamlensis sp. nov., isolated from seawater. Int J Syst Evol Microbiol 2012; 62:586–590 [View Article][PubMed]
     [Google Scholar]
  25. Tsubouchi T, Shimane Y, Mori K, Miyazaki M, Tame A et al. Loktanella cinnabarina sp. nov., isolated from a deep subseafloor sediment, and emended description of the genus Loktanella . Int J Syst Evol Microbiol 2013; 63:1390–1395 [View Article][PubMed]
     [Google Scholar]
  26. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101. Newark, DE: MIDI Inc; 1990
     [Google Scholar]
  27. Minnikin DE, O'Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984; 2:233–241 [View Article]
     [Google Scholar]
  28. Embley TM, Wait R. Structural lipids of eubacteria. In Goodfellow M, O’Donnell AG. (editors) Modern Microbial Methods. Chemical Methods in Prokaryotic Systematics Chichester: John Wiley & Sons; 1994 pp. 121–161
     [Google Scholar]
  29. Tamaoka J, Komagata K. Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 1984; 25:125–128 [View Article]
     [Google Scholar]
  30. Wayne LG, Moore WEC, Stackebrandt E, Kandler O, Colwell RR et al. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Evol Microbiol 1987; 37:463–464 [View Article]
     [Google Scholar]
  31. Stackebrandt E, Goebel BM. Taxonomic note: A place for DNA–DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Evol Microbiol 1994; 44:846–849 [View Article]
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.002274
Loading
Loktanella acticola sp. nov., isolated from seawater
Int J Syst Evol Microbiol 67, 4175 (2017); https://doi.org/10.1099/ijsem.0.002274
/content/journal/ijsem/10.1099/ijsem.0.002274
/content/journal/ijsem/10.1099/ijsem.0.002274
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error