1887

Abstract

A nitrogen-fixing bacterium, designated NF2-4-5, was isolated from a paddy soil in Anseong City, Korea. Cells of strain NF2-4-5 were Gram-staining-positive, motile rods and aerobic. Phylogenetic analysis based on 16S rRNA gene sequence comparison revealed that the strain formed a distinct lineage within the genus and was closely related to RSA19 (98.7 %), Be17 (98.6 %), LAM0A28 (98.6 %) and SBR5 (98.6 %). Growth of strain NF2-4-5 occurs at temperatures of 18–37 °C, at pH 6.0–8.5 and between 0.5% and 2 % NaCl (w/v). The only respiratory quinone was MK-7. The cell wall peptidoglycan of strain NF2-4-5 contained meso-diaminopimelic acid. The main cellular fatty acids were C and anteiso-C. The major polar lipids were diphosphatidylglycerol (DPG), phosphatidylglycerol (PG) and phosphatidylethanolamine (PE). The DNA G+C content was 56.36 mol%. The DNA–DNA hybridization relatedness between strain NF2-4-5 and four reference strains, RSA19, Be17, X19-5 and SBR5, was 22.0±0.3, 20.1±0.7, 18.3±0.3 and 12.6±0.5 %, respectively. The phenotypic, phylogenetic and chemotaxonomic results indicate that the strain NF2-4-5 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is NF2-4-5 (=KACC 18967=LMG 29963).

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2017-12-01
2019-12-13
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