1887

Abstract

A strictly anaerobic bacterial strain (SH021) was isolated from a methanogenic reactor. Cells were Gram-stain-positive, motile, straight or slightly curved rods. The optimum temperature for growth was 35 °C, and the optimum pH was 6.1–7.7. The strain was asaccharolytic and utilized amino acids as growth substrates. The strain produced acetate and propionate from -alanine and -serine, and propionate and butyrate from -threonine. Branched-chain amino acids (-isoleucine, -leucine and -valine) were utilized weakly, and isovalerate or isobutyrate was produced. Strain SH021 utilized pyruvate and lactate, and converted them to acetate and propionate. The genomic DNA G+C content was 38.2 mol%. Compounds related to iso-C were detected as major components in the cellular fatty acids analysis. The diagnostic diamino acid of the cell-wall peptidoglycan was -diaminopimelic acid. On the basis of 16S rRNA gene sequences, the most closely related known species were , and in cluster XIVb of the class . Based on the phylogenetic and phenotypic data, gen. nov., sp. nov. is proposed to accommodate strain SH021 (=JCM 31556=DSM 103575). For the three related species of the genus , comb. nov. (type strain DSM 1682=JCM 1430=ATCC 25522=CCUG 9280=NCIMB 10656=VPI 5303), comb. nov. (type strain X4=DSM 3847=KCTC 15564) and comb. nov. (type strain G17=DSM 14214=LMG 20954) are proposed with emended descriptions of these species.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.002268
2017-10-01
2019-12-11
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/67/10/4146.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.002268&mimeType=html&fmt=ahah

References

  1. Buckel W. Anaerobic energy metabolism. In Lengeler JW, Drews G, Schlegel HG. (editors) Biology of the Prokaryotes Stuttgart: Blackwell Science; 1999; pp.278–326
    [Google Scholar]
  2. Rainey FA, Hollen BJ, Small A. Genus I. Clostridium Prazmowski 1880, 23AL. In PDe Vos, Garrity G, Jones D, Krieg NR, Ludwig W et al. (editors) Bergey’s Manual of Systematic Bacteriology, 2nd ed.vol.3 New York: Springer; pp.736–864
    [Google Scholar]
  3. Ramsay IR, Pullammanappallil PC. Protein degradation during anaerobic wastewater treatment: derivation of stoichiometry. Biodegradation 2001;12:247–256 [CrossRef][PubMed]
    [Google Scholar]
  4. Smith EA, Macfarlane GT. Dissimilatory amino acid metabolism in human colonic bacteria. Anaerobe 1997;3:327–337 [CrossRef][PubMed]
    [Google Scholar]
  5. Ueki A, Abe K, Suzuki D, Kaku N, Watanabe K et al. Anaerosphaera aminiphila gen. nov., sp. nov., a glutamate-degrading, Gram-positive anaerobic coccus isolated from a methanogenic reactor treating cattle waste. Int J Syst Evol Microbiol 2009;59:3161–3167 [CrossRef][PubMed]
    [Google Scholar]
  6. Ueki A, Shibuya T, Kaku N, Ueki K. Aminocella lysinolytica gen. nov., sp. nov., a L-lysine-degrading, strictly anaerobic bacterium in the class Clostridia isolated from a methanogenic reactor of cattle farms. Arch Microbiol 2015;197:97–104 [CrossRef][PubMed]
    [Google Scholar]
  7. Watanabe M, Kaku N, Ueki K, Ueki A. Falcatimonas natans gen. nov., sp. nov., a strictly anaerobic, amino-acid-decomposing bacterium isolated from a methanogenic reactor of cattle waste. Int J Syst Evol Microbiol 2016;66:4639–4644 [CrossRef][PubMed]
    [Google Scholar]
  8. Collins MD, Lawson PA, Willems A, Cordoba JJ, Fernandez-Garayzabal J et al. The phylogeny of the genus Clostridium: proposal of five new genera and eleven new species combinations. Int J Syst Bacteriol 1994;44:812–826 [CrossRef][PubMed]
    [Google Scholar]
  9. Cardon BP, Barker HA. Two new amino-acid-fermenting bacteria, Clostridium propionicum and Diplococcus glycinophilus. J Bacteriol 1946;52:629–634[PubMed]
    [Google Scholar]
  10. Tholozan JL, Touzel JP, Samain E, Grivet JP, Prensier G et al. Clostridium neopropionicum sp. nov., a strict anaerobic bacterium fermenting ethanol to propionate through acrylate pathway. Arch Microbiol 1992;157:249–257 [CrossRef][PubMed]
    [Google Scholar]
  11. van der Wielen PW, Rovers GM, Scheepens JM, Biesterveld S. Clostridium lactatifermentans sp. nov., a lactate-fermenting anaerobe isolated from the caeca of a chicken. Int J Syst Evol Microbiol 2002;52:921–925 [CrossRef][PubMed]
    [Google Scholar]
  12. Cai S, Dong X. Cellulosilyticum ruminicola gen. nov., sp. nov., isolated from the rumen of yak, and reclassification of Clostridium lentocellum as Cellulosilyticum lentocellum comb. nov. Int J Syst Evol Microbiol 2010;60:845–849 [CrossRef][PubMed]
    [Google Scholar]
  13. Berkhoff HA. Clostridium colinum sp. nov., nom. rev., the causative agent of ulcerative enteritis (quail disease) in quail, chickens, and pheasants. Int J Syst Bacteriol 1985;35:155–159 [CrossRef]
    [Google Scholar]
  14. Lawson PA, Rainey FA. Proposal to restrict the genus Clostridium Prazmowski to Clostridium butyricum and related species. Int J Syst Evol Microbiol 2016;66:1009–1016 [CrossRef][PubMed]
    [Google Scholar]
  15. Abe K, Ueki A, Ohtaki Y, Kaku N, Watanabe K et al. Anaerocella delicata gen. nov., sp. nov., a strictly anaerobic bacterium in the phylum Bacteroidetes isolated from a methanogenic reactor of cattle farms. J Gen Appl Microbiol 2012;58:405–412 [CrossRef][PubMed]
    [Google Scholar]
  16. Satoh A, Watanabe M, Ueki A, Ueki K. Physiological properties and phylogenetic affiliations of anaerobic bacteria isolated from roots of rice plants cultivated on a paddy field. Anaerobe 2002;8:233–246 [CrossRef]
    [Google Scholar]
  17. Blenden DC, Goldberg HS. Silver impregnation stain for leptospira and flagella. J Bacteriol 1965;89:899–900[PubMed]
    [Google Scholar]
  18. Holdeman LV, Cato EP, Moore WEC. Anaerobe Laboratory Manual, 4th ed. Blacksburg, VA: Virginia Polytechnic Institute and State University; 1977
    [Google Scholar]
  19. Akasaka H, Ueki A, Hanada S, Kamagata Y, Ueki K. Propionicimonas paludicola gen. nov., sp. nov., a novel facultatively anaerobic, Gram-positive, propionate-producing bacterium isolated from plant residue in irrigated rice-field soil. Int J Syst Evol Microbiol 2003;53:1991–1998 [CrossRef][PubMed]
    [Google Scholar]
  20. Miller LT. Single derivatization method for routine analysis of bacterial whole-cell fatty acid methyl esters, including hydroxy acids. J Clin Microbiol 1982;16:584–586[PubMed]
    [Google Scholar]
  21. Moore LV, Bourne DM, Moore WE. Comparative distribution and taxonomic value of cellular fatty acids in thirty-three genera of anaerobic gram-negative bacilli. Int J Syst Bacteriol 1994;44:338–347 [CrossRef][PubMed]
    [Google Scholar]
  22. Komagata K, Suzuki K. Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 1987;19:161–207[Crossref]
    [Google Scholar]
  23. Brosius J, Dull TJ, Sleeter DD, Noller HF. Gene organization and primary structure of a ribosomal RNA operon from Escherichia coli. J Mol Biol 1981;148:107–127 [CrossRef][PubMed]
    [Google Scholar]
  24. Altschul SF, Madden TL, Schäffer AA, Zhang J, Zhang Z et al. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 1997;25:3389–3402 [CrossRef][PubMed]
    [Google Scholar]
  25. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987;4:406–425[PubMed]
    [Google Scholar]
  26. Thompson JD, Higgins DG, Gibson TJ. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 1994;22:4673–4680 [CrossRef][PubMed]
    [Google Scholar]
  27. Yarza P, Yilmaz P, Pruesse E, Glöckner FO, Ludwig W et al. Uniting the classification of cultured and uncultured bacteria and archaea using 16S rRNA gene sequences. Nat Rev Microbiol 2014;12:635–645 [CrossRef][PubMed]
    [Google Scholar]
  28. Yutin N, Galperin MY. A genomic update on clostridial phylogeny: Gram-negative spore formers and other misplaced clostridia. Environ Microbiol 2013;15:n/a–2641 [CrossRef][PubMed]
    [Google Scholar]
  29. Skerman VBD, Sneath PHA, Mcgowan V. Approved lists of bacterial names. Int J Syst Evol Microbiol 1980;30:225–420 [CrossRef]
    [Google Scholar]
  30. Tholozan JL, Touzel JP, Samain E, Grivet JP, Prensier G et al. In Validation of the publication of new names and new combinations previously effectively published outside the IJSB. List no. 55. Int J Syst Bacteriol 1995;45:879–880[Crossref]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.002268
Loading
/content/journal/ijsem/10.1099/ijsem.0.002268
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error