1887

Abstract

A Gram-stain positive, aerobic, non-motile, rod-shaped bacterium (THG-T1.18) was isolated from desert soil. Growth occurred at 20–35 °C (optimum 28–30 °C), at pH 5–7 (optimum 7) and at 0–4 % NaCl (optimum 0–1 %). Based on 16S rRNA sequence analysis, the nearest phylogenetic neighbours of strain THG-T1.18 were identified as Chryseomicrobium amylolyticum DSM 23442 (96.6 %), Chryseomicrobium imtechense JCM 16573 (96.3 %) and Chryseomicrobium aureum KACC 17219 (96.1 %). The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, two unidentified aminolipids and one unidentified glycolipid. The quinone system was composed of MK-7, MK-8 and MK-6. The major fatty acids were iso C15 : 0 and anteiso C15 : 0. The type of peptidoglycan was A4β, containing of l-Orn–D-Glu. The DNA G+C content of strain THG-T1.18 was 50.4 mol%. DNA–DNA hybridization values between strain THG-T1.18 and C. amylolyticum DSM 23442, C. imtechense JCM 16573, C. aureum KACC 17219 were 24.7 % (20.1 % reciprocal analysis), 19.5 % (16.1 %) and 10.4 % (6.7 %) respectively. On the basis of the phylogenetic analysis, chemotaxonomic data, physiological characteristics and DNA–DNA hybridization data, strain THG-T1.18 represents a novel species of the genus Chryseomicrobium , for which the name Chryseomicrobium deserti sp. nov. is proposed. The type strain is THG-T1.18 (=KACC 18929=CCTCC AB 2016179).

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2017-09-13
2019-12-07
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