1887

Abstract

An alkaliphilic and moderately halophilic bacterial strain characterized by optimal growth at pH 9.0–10.0 and with 5–7 % (w/v) NaCl, designated BV-35, was isolated from water of a soda pan located in Kiskunság National Park, Hungary. Cells of the orange-pigmented colony were Gram-stain-positive, non-motile and non-endospore-forming coccoid rods. The isolate was strictly aerobic, catalase-positive and oxidase-negative. Strain BV-35 displayed a peptidoglycan similar to type A4α, l-Lys–l-Glu (A11.54 according to www.peptidoglycan-types.info) but containing additionally 4-aminobutyric acid. Menaquinone-7 (MK-7) was the predominant isoprenoid quinone, and anteiso-C15 : 0 and anteiso-C17 : 0 were its major cellular fatty acids. The DNA G+C content of strain BV-35 was 65.4 mol%. Based on 16S rRNA gene sequence similarities, the novel isolate showed the closest relationship to Nesterenkonia populi GP 10-3 (97.9 %). The DNA–DNA relatedness between BV-35 and N. populi was 46.7 %. The distinguishing phenotypic and genetic results of this polyphasic study revealed that strain BV-35 represents a novel member of the genus Nesterenkonia , for which the name Nesterenkonia pannonica sp. nov. is proposed. The type strain is BV-35 (=DSM 29786=NCAIM B 02606).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.002263
2017-09-14
2019-12-06
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/67/10/4116.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.002263&mimeType=html&fmt=ahah

References

  1. Stackebrandt E, Koch C, Gvozdiak O, Schumann P. Taxonomic dissection of the genus Micrococcus: Kocuria gen. nov., Nesterenkonia gen. nov., Kytococcus gen. nov., Dermacoccus gen. nov., and Micrococcus Cohn 1872 gen. emend. Int J Syst Bacteriol 1995; 45: 682– 692 [CrossRef] [PubMed]
    [Google Scholar]
  2. Collins MD, Lawson PA, Labrenz M, Tindall BJ, Weiss N et al. Nesterenkonia lacusekhoensis sp. nov., isolated from hypersaline Ekho Lake, East Antarctica, and emended description of the genus Nesterenkonia. Int J Syst Evol Microbiol 2002; 52: 1145– 1150 [CrossRef] [PubMed]
    [Google Scholar]
  3. Li WJ, Chen HH, Kim CJ, Zhang YQ, Park DJ et al. Nesterenkonia sandarakina sp. nov. and Nesterenkonia lutea sp. nov., novel actinobacteria, and emended description of the genus Nesterenkonia. Int J Syst Evol Microbiol 2005; 55: 463– 466 [CrossRef] [PubMed]
    [Google Scholar]
  4. Mota RR, Márquez MC, Arahal DR, Mellado E, Ventosa A. Polyphasic taxonomy of Nesterenkonia halobia. Int J Syst Bacteriol 1997; 47: 1231– 1235 [CrossRef] [PubMed]
    [Google Scholar]
  5. Govender L, Naidoo L, Setati ME. Nesterenkonia suensis sp. nov., a haloalkaliphilic actinobacterium isolated from a salt pan. Int J Syst Evol Microbiol 2013; 63: 41– 46 [CrossRef] [PubMed]
    [Google Scholar]
  6. Delgado O, Quillaguamán J, Bakhtiar S, Mattiasson B, Gessesse A et al. Nesterenkonia aethiopica sp. nov., an alkaliphilic, moderate halophile isolated from an Ethiopian soda lake. Int J Syst Evol Microbiol 2006; 56: 1229– 1232 [CrossRef] [PubMed]
    [Google Scholar]
  7. Zhang G, Zhang Y, Yin X, Wang S. Nesterenkonia alkaliphila sp. nov., an alkaliphilic, halotolerant actinobacteria isolated from the western Pacific Ocean. Int J Syst Evol Microbiol 2015; 65: 516– 521 [CrossRef] [PubMed]
    [Google Scholar]
  8. Li WJ, Chen HH, Zhang YQ, Schumann P, Stackebrandt E et al. Nesterenkonia halotolerans sp. nov. and Nesterenkonia xinjiangensis sp. nov., actinobacteria from saline soils in the west of China. Int J Syst Evol Microbiol 2004; 54: 837– 841 [CrossRef] [PubMed]
    [Google Scholar]
  9. Li WJ, Zhang YQ, Schumann P, Liu HY, Yu LY et al. Nesterenkonia halophila sp. nov., a moderately halophilic, alkalitolerant actinobacterium isolated from a saline soil. Int J Syst Evol Microbiol 2008; 58: 1359– 1363 [CrossRef] [PubMed]
    [Google Scholar]
  10. Wang HF, Zhang YG, Chen JY, Hozzein WN, Li L et al. Nesterenkonia rhizosphaerae sp. nov., an alkaliphilic actinobacterium isolated from rhizosphere soil in a saline-alkaline desert. Int J Syst Evol Microbiol 2014; 64: 4021– 4026 [CrossRef] [PubMed]
    [Google Scholar]
  11. Finore I, di Donato P, Nicolaus B, Orlando P, Leone L et al. Nesterenkonia aurantiaca sp. nov., an alkaliphilic actinobacterium isolated from Cape King (Antarctica). Int J Syst Evol Microbiol 2016; 66: 1554– 1560 [CrossRef]
    [Google Scholar]
  12. Yoon JH, Jung SY, Kim W, Nam SW, Oh TK et al. Nesterenkonia jeotgali sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. Int J Syst Evol Microbiol 2006; 56: 2587– 2592 [CrossRef] [PubMed]
    [Google Scholar]
  13. Luo HY, Miao LH, Fang C, Yang PL, Wang YR et al. Nesterenkonia flava sp. nov., isolated from paper-mill effluent. Int J Syst Evol Microbiol 2008; 58: 1927– 1930 [CrossRef] [PubMed]
    [Google Scholar]
  14. Luo HY, Wang YR, Miao LH, Yang PL, Shi PJ et al. Nesterenkonia alba sp. nov., an alkaliphilic actinobacterium isolated from the black liquor treatment system of a cotton pulp mill. Int J Syst Evol Microbiol 2009; 59: 863– 868 [CrossRef] [PubMed]
    [Google Scholar]
  15. Edouard S, Sankar S, Dangui NP, Lagier JC, Michelle C et al. Genome sequence and description of Nesterenkonia massiliensis sp. nov. strain NP1T. Stand Genomic Sci 2014; 9: 866– 882 [CrossRef] [PubMed]
    [Google Scholar]
  16. Liu JM, Tuo L, Habden X, Guo L, Jiang ZK et al. Nesterenkonia populi sp. nov., an actinobacterium isolated from Populus euphratica. Int J Syst Evol Microbiol 2015; 65: 1474– 1479 [CrossRef] [PubMed]
    [Google Scholar]
  17. Boros E, Horváth Z, Wolfram G, Vörös L. Salinity and ionic composition of the shallow astatic soda pans in the Carpathian Basin. Ann Limnol - Int J Lim 2014; 50: 59– 69 [CrossRef]
    [Google Scholar]
  18. Claus D. A standardized Gram staining procedure. World J Microbiol Biotechnol 1992; 8: 451– 452 [CrossRef] [PubMed]
    [Google Scholar]
  19. Barrow GI, Feltham RKA. Cowan and Steel’s Manual for the Identification of Medical Bacteria Cambridge: Cambridge University Press; 2003
    [Google Scholar]
  20. Smibert RM, Krieg NR. Phenotypic characterization. In Gerhardt P, Murray RGE, Wood WA, Krieg NR. (editors) Methods for General and Molecular Bacteriology Washington, DC: ASM; 1994; pp. 603– 711
    [Google Scholar]
  21. Schumann P. Peptidoglycan structure. Methods Microbiol 2011; 38: 101– 129 [Crossref]
    [Google Scholar]
  22. Takeuchi M, Weiss N, Schumann P, Yokota A. Leucobacter komagatae gen. nov., sp. nov., a new aerobic gram-positive, nonsporulating rod with 2,4-diaminobutyric acid in the cell wall. Int J Syst Bacteriol 1996; 46: 967– 971 [CrossRef] [PubMed]
    [Google Scholar]
  23. Lin YC, Uemori K, de Briel DA, Arunpairojana V, Yokota A. Zimmermannella helvola gen. nov., sp. nov., Zimmermannella alba sp. nov., Zimmermannella bifida sp. nov., Zimmermannella faecalis sp. nov. and Leucobacter albus sp. nov., novel members of the family Microbacteriaceae. Int J Syst Evol Microbiol 2004; 54: 1669– 1676 [CrossRef] [PubMed]
    [Google Scholar]
  24. Weon HY, Anandham R, Tamura T, Hamada M, Kim SJ et al. Leucobacter denitrificans sp. nov., isolated from cow dung. J Microbiol 2012; 50: 161– 165 [CrossRef] [PubMed]
    [Google Scholar]
  25. Halpern M, Shakéd T, Pukall R, Schumann P. Leucobacter chironomi sp. nov., a chromate-resistant bacterium isolated from a chironomid egg mass. Int J Syst Evol Microbiol 2009; 59: 665– 670 [CrossRef] [PubMed]
    [Google Scholar]
  26. Yun JH, Roh SW, Kim MS, Jung MJ, Park EJ et al. Leucobacter salsicius sp. nov., from a salt-fermented food. Int J Syst Evol Microbiol 2011; 61: 502– 506 [CrossRef] [PubMed]
    [Google Scholar]
  27. Lee JH, Lee SS. Leucobacter margaritiformis sp. nov., isolated from bamboo extract. Curr Microbiol 2012; 64: 441– 448 [CrossRef] [PubMed]
    [Google Scholar]
  28. Ue H. Leucobacter exalbidus sp. nov., an actinobacterium isolated from a mixed culture from compost. J Gen Appl Microbiol 2011; 57: 27– 33 [CrossRef] [PubMed]
    [Google Scholar]
  29. Borsodi AK, Pollák B, Kéki Z, Rusznyák A, Kovács AL et al. Bacillus alkalisediminis sp. nov., an alkaliphilic and moderately halophilic bacterium isolated from sediment of extremely shallow soda ponds. Int J Syst Evol Microbiol 2011; 61: 1880– 1886 [CrossRef] [PubMed]
    [Google Scholar]
  30. Schumann P, Maier T. MALDI-TOF mass spectrometry applied to classification and identification of bacteria. Methods Microbiol 2014; 41: 275– 306 [Crossref]
    [Google Scholar]
  31. Soergel DA, Dey N, Knight R, Brenner SE. Selection of primers for optimal taxonomic classification of environmental 16S rRNA gene sequences. Isme J 2012; 6: 1440– 1444 [CrossRef] [PubMed]
    [Google Scholar]
  32. Yoon SH, Ha SM, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017; 67: 1613– 1617 [CrossRef] [PubMed]
    [Google Scholar]
  33. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013; 30: 2725– 2729 [CrossRef] [PubMed]
    [Google Scholar]
  34. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4: 406– 425 [PubMed]
    [Google Scholar]
  35. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981; 17: 368– 376 [CrossRef] [PubMed]
    [Google Scholar]
  36. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980; 16: 111– 120 [CrossRef] [PubMed]
    [Google Scholar]
  37. Wayne LG, Moore WEC, Stackebrandt E, Kandler O, Colwell RR et al. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Evol Microbiol 1987; 37: 463– 464 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.002263
Loading
/content/journal/ijsem/10.1099/ijsem.0.002263
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error