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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 67, Issue 10

sp. nov., a novel alkaliphilic and moderately halophilic actinobacterium Free

Abstract

An alkaliphilic and moderately halophilic bacterial strain characterized by optimal growth at pH 9.0–10.0 and with 5–7 % (w/v) NaCl, designated BV-35, was isolated from water of a soda pan located in Kiskunság National Park, Hungary. Cells of the orange-pigmented colony were Gram-stain-positive, non-motile and non-endospore-forming coccoid rods. The isolate was strictly aerobic, catalase-positive and oxidase-negative. Strain BV-35 displayed a peptidoglycan similar to type A4α, -Lys–-Glu (A11.54 according to www.peptidoglycan-types.info) but containing additionally 4-aminobutyric acid. Menaquinone-7 (MK-7) was the predominant isoprenoid quinone, and anteiso-C and anteiso-C were its major cellular fatty acids. The DNA G+C content of strain BV-35 was 65.4 mol%. Based on 16S rRNA gene sequence similarities, the novel isolate showed the closest relationship to GP 10-3 (97.9 %). The DNA–DNA relatedness between BV-35 and was 46.7 %. The distinguishing phenotypic and genetic results of this polyphasic study revealed that strain BV-35 represents a novel member of the genus , for which the name sp. nov. is proposed. The type strain is BV-35 (=DSM 29786=NCAIM B 02606).

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Keyword(s): alkaliphilic , moderately halophilic , Nesterenkonia and soda pan
© 2017 IUMS
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2017-10-01
2024-04-20
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Nesterenkonia pannonica sp. nov., a novel alkaliphilic and moderately halophilic actinobacterium
Int J Syst Evol Microbiol 67, 4116 (2017); https://doi.org/10.1099/ijsem.0.002263
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