1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 67, Issue 10

gen. nov., sp. nov., a novel psychrotolerant chitinolytic anaerobe of the family Free

Abstract

A psychrotolerant chitinolytic bacterium, designated NC1253, was isolated from Zoige wetland on the Qinghai-Tibetan Plateau. This strain was a Gram-stain-positive, spore-forming and rod-shaped anaerobe. NC1253 grew at 4–35 °C, at pH 6.0–8.5 and could grow on chitin as the only carbon resource. Phylogenetic analysis, based on the 16S rRNA gene sequence, showed that strain NC1253 represented a novel bacterial genus within the family . Strain NC1253 has less than 91.0 % similarity with other type strains, such as V20-281a (90.9 %), DSM 5476 (90.8 %), DSM 17241 (89.8 %), DSM 15702 (89.6 %), and Z7 (89.6 %). The major components of the cellular fatty acids were iso-C, anteiso-C, C and anteiso-C. The genomic DNA G+C content was 35.4 mol%. Phenotypic, chemotaxonomic and phylogenetic characteristics allowed strain NC1253 to be clearly distinguished from genera in the family . On the basis of polyphasic taxonomic data, the isolate is considered to represent a novel genus and novel species in the family , for which the name gen. nov., sp. nov., is proposed. The type species is NC1253 (DSM 104738=KCTC 15582).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): chitinolysis , Paludicola psychrotolerans , psychrotroph , Qinghai-Tibetan Plateau and wetland soil
© 2017 IUMS
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.002260
2017-10-01
2024-04-18
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/67/10/4100.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.002260&mimeType=html&fmt=ahah

References

  1. Shankar S, Reddy JP, Rhim JW, Kim HY. Preparation, characterization, and antimicrobial activity of chitin nanofibrils reinforced carrageenan nanocomposite films. Carbohydr Polym 2015; 117:468–475 [View Article][PubMed]
     [Google Scholar]
  2. Kang H, Freeman C, Soon Park S, Chun J. N-Acetylglucosaminidase activities in wetlands: a global survey. Hydrobiologia 2005; 532:103–110 [View Article]
     [Google Scholar]
  3. Wieczorek AS, Hetz SA, Kolb S. Microbial responses to chitin and chitosan in oxic and anoxic agricultural soil slurries. Biogeosciences 2014; 11:3339–3352 [View Article]
     [Google Scholar]
  4. Rainey FA. Family VIII. Ruminococcaceae fam. nov. In De Vos P, Garrity GM, Jones D, Krieg NR, Ludwig W. et al (editors) Bergey's Manual of Systematic Bacteriology, 2nd ed. vol.3 (The Firmicutes) Dordrecht, Heidelberg, London, New York: Springer; 2009 pp. 1016
     [Google Scholar]
  5. Li X, Luo Q, Wofford NQ, Keller KL, Mclnerney MJ et al. A molybdopterin oxidoreductase is involved in H2 oxidation in Desulfovibrio desulfuricans G20. J Bacteriol 2009; 191:2675–2682 [View Article][PubMed]
     [Google Scholar]
  6. Shen CR, Chen YS, Yang CJ, Chen JK, Liu CL. Colloid chitin azure is a dispersible, low-cost substrate for chitinase measurements in a sensitive, fast, reproducible assay. J Biomol Screen 2010; 15:213–217 [View Article][PubMed]
     [Google Scholar]
  7. Lane DJ. 16S/23S rRNA sequencing. In Stackebrandt E, Goodfellow M. (editors) Nucleic Acid Techniques in Bacterial Systematics New York: Wiley; 1991 pp. 115–175
     [Google Scholar]
  8. Yoon SH, Ha SM, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017; 67:1613–1617 [View Article][PubMed]
     [Google Scholar]
  9. Jeon YS, Lee K, Park SC, Kim BS, Cho YJ et al. EzEditor: a versatile sequence alignment editor for both rRNA- and protein-coding genes. Int J Syst Evol Microbiol 2014; 64:689–691 [View Article][PubMed]
     [Google Scholar]
  10. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980; 16:111–120 [View Article][PubMed]
     [Google Scholar]
  11. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 2016; 33:1870–1874 [View Article][PubMed]
     [Google Scholar]
  12. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985; 39:783–791 [View Article][PubMed]
     [Google Scholar]
  13. Mesbah M, Premachandran U, Whitman WB. Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 1989; 39:159–167 [View Article]
     [Google Scholar]
  14. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101. Newark, DE: MIDI Inc; 1990
     [Google Scholar]
  15. Lawson PA, Song Y, Liu C, Molitoris DR, Vaisanen ML et al. Anaerotruncus colihominis gen. nov., sp. nov., from human faeces. Int J Syst Evol Microbiol 2004; 54:413–417 [View Article][PubMed]
     [Google Scholar]
  16. Himelbloom BH, Canale-Parola E. Clostridium methylpentosum sp. nov.: a ring-shaped intestinal bacterium that ferments only methylpentoses and pentoses. Arch Microbiol 1989; 151:287–293 [View Article][PubMed]
     [Google Scholar]
  17. Petzoldt D, Breves G, Rautenschlein S, Taras D. Harryflintia acetispora gen. nov., sp. nov., isolated from chicken caecum. Int J Syst Evol Microbiol 2016; 66:4099–4104 [View Article][PubMed]
     [Google Scholar]
  18. Moore WEC, Johnson JL, Holdeman LV. Emendation of Bacteroidaceae and Butyrivibrio and descriptions of Desulfomonas gen. nov. and ten new species in the genera Desulfomonas, Butyrivibrio, Eubacterium, Clostridium, and Ruminococcus. Int J Syst Bacteriol 1976; 26:238–252 [View Article]
     [Google Scholar]
  19. Chen S, Dong X. Acetanaerobacterium elongatum gen. nov., sp. nov., from paper mill waste water. Int J Syst Evol Microbiol 2004; 54:2257–2262 [View Article][PubMed]
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.002260
Loading
Paludicola psychrotolerans gen. nov., sp. nov., a novel psychrotolerant chitinolytic anaerobe of the family Ruminococcaceae
Int J Syst Evol Microbiol 67, 4100 (2017); https://doi.org/10.1099/ijsem.0.002260
/content/journal/ijsem/10.1099/ijsem.0.002260
/content/journal/ijsem/10.1099/ijsem.0.002260
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error