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Abstract

A psychrotolerant chitinolytic bacterium, designated NC1253, was isolated from Zoige wetland on the Qinghai-Tibetan Plateau. This strain was a Gram-stain-positive, spore-forming and rod-shaped anaerobe. NC1253 grew at 4–35 °C, at pH 6.0–8.5 and could grow on chitin as the only carbon resource. Phylogenetic analysis, based on the 16S rRNA gene sequence, showed that strain NC1253 represented a novel bacterial genus within the family Ruminococcaceae . Strain NC1253 has less than 91.0 % similarity with other type strains, such as Harryflintia acetispora V20-281a (90.9 %), Clostridium methylpentosum DSM 5476 (90.8 %), Anaerotruncus colihominis DSM 17241 (89.8 %), Eubacterium siraeum DSM 15702 (89.6 %), and Acetanaerobacterium elongatum Z7 (89.6 %). The major components of the cellular fatty acids were iso-C14 : 0, anteiso-C15 : 0, C16 : 0 and anteiso-C17 : 0. The genomic DNA G+C content was 35.4 mol%. Phenotypic, chemotaxonomic and phylogenetic characteristics allowed strain NC1253 to be clearly distinguished from genera in the family Ruminococcaceae . On the basis of polyphasic taxonomic data, the isolate is considered to represent a novel genus and novel species in the family Ruminococcaceae , for which the name Paludicola psychrotolerans gen. nov., sp. nov., is proposed. The type species is NC1253 (DSM 104738=KCTC 15582).

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2017-09-13
2019-10-21
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