1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 67, Issue 10

sp. nov., isolated from a marine solar saltern Free

Abstract

Halophilic archaeal strain ZS-54-S2 was isolated from Zhoushan marine solar saltern, China. Cells were rod-shaped, Gram-stain-negative and formed red-pigmented colonies on an agar plate. Strain ZS-54-S2 was able to grow at 20–50 °C (optimum 35 °C), at 1.7–4.8 M NaCl (optimum 3.9 M), at 0.005–1.0 M MgCl (optimum 0.05 M) and at pH 5.0–9.5 (optimum pH 7.0). The cells lysed in distilled water and the minimal NaCl concentration to prevent cell lysis was found to be 5 % (w/v). The major polar lipids of the strain were phosphatidic acid, phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, two glycolipids, which were chromatographically identical to sulfated galactosyl mannosyl galactofuranosyl glucosyl diether and galactosyl mannosyl glucosyl diether, and an unidentified glycolipid, which was chromatographically identical to one detected in ATCC 33171. The 16S rRNA gene and gene of strain ZS-54-S2 were phylogenetically related to the corresponding genes of JCM 15102 (97.5 % and 90.6 % relatedness, respectively), CGMCC 1.5337 (96.9 and 91.2 %), CGMCC 1.12575 (96.8 and 90.3 %) and CGMCC 1.1958 (96.5 and 88.4 %). The DNA G+C content of strain ZS-54-S2 was 66.7 mol%. The phenotypic, chemotaxonomic and phylogenetic properties suggested that strain ZS-54-S2 (=CGMCC 1.12562=JCM 30038) represents a new species of , for which the name sp. nov. is proposed.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Halobacterium litoreum sp. nov. , halophilic archaeon and marine solar saltern
© 2017 IUMS
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.002259
2017-10-01
2024-04-20
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/67/10/4095.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.002259&mimeType=html&fmt=ahah

References

  1. Oren A. Taxonomy of Halophilic archaea: current status and future challenges. Extremophiles 2014; 18:825–834 [View Article][PubMed]
     [Google Scholar]
  2. Gupta RS, Naushad S, Baker S. Phylogenomic analyses and molecular signatures for the class Halobacteria and its two major clades: a proposal for division of the class Halobacteria into an emended order Halobacteriales and two new orders, Haloferacales ord. nov. and Natrialbales ord. nov., containing the novel families Haloferacaceae fam. nov. and Natrialbaceae fam. nov. Int J Syst Evol Microbiol 2015; 65:1050–1069 [View Article][PubMed]
     [Google Scholar]
  3. Elazari-Volcani B, Genus XII. Genus XII. Halobacterium Elazari-Volcani, 1940. In Breed RS, Murrry EGD, Smith NR. (editors) Bergey’s Manual of Determinative Bacteriology, 7th ed. Baltimore: Williams and Wilkins; 1957 pp. 207–212
     [Google Scholar]
  4. Ventosa A, Oren A, Nom.Corrig H. Halobacterium salinarum nom. corrig., a name to replace Halobacterium salinarium (Elazari-Volcani) and to include Halobacterium halobium and Halobacterium cutirubrum. Int J Syst Bacteriol 1996; 46:347 [View Article]
     [Google Scholar]
  5. Gruber C, Legat A, Pfaffenhuemer M, Radax C, Weidler G et al. Halobacterium noricense sp. nov., an archaeal isolate from a bore core of an alpine permian salt deposit, classification of Halobacterium sp. NRC-1 as a strain of H. salinarum and emended description of H. salinarum. Extremophiles 2004; 8:431–439 [View Article][PubMed]
     [Google Scholar]
  6. Yang Y. Halobacterium jilantaiense sp. nov., a halophilic archaeon isolated from a saline lake in Inner Mongolia, China. Int J Syst Evol Microbiol 2006; 56:2353–2355 [View Article]
     [Google Scholar]
  7. Han D, Cui HL. Halobacterium rubrum sp. nov., isolated from a marine solar saltern. Arch Microbiol 2014; 196:847–851 [View Article][PubMed]
     [Google Scholar]
  8. Oren A, Arahal DR, Ventosa A. Emended descriptions of genera of the family Halobacteriaceae. Int J Syst Evol Microbiol 2009; 59:637–642 [View Article][PubMed]
     [Google Scholar]
  9. Oren A, Ventosa A, Grant WD. Proposed minimal standards for description of new taxa in the order Halobacteriales. Int J Syst Bacteriol 1997; 47:233–238 [View Article]
     [Google Scholar]
  10. Cui H-L, Gao X, Yang X, Xu X-W. Halorussus rarus gen. nov., sp. nov., a new member of the family Halobacteriaceae isolated from a marine solar saltern. Extremophiles 2010; 14:493–499 [View Article]
     [Google Scholar]
  11. Cui HL, Zhou PJ, Oren A, Liu SJ. Intraspecific polymorphism of 16S rRNA genes in two halophilic archaeal genera, Haloarcula and Halomicrobium. Extremophiles 2009; 13:31–37 [View Article][PubMed]
     [Google Scholar]
  12. Minegishi H, Kamekura M, Itoh T, Echigo A, Usami R et al. Further refinement of the phylogeny of the Halobacteriaceae based on the full-length RNA polymerase subunit B' (rpoB') gene. Int J Syst Evol Microbiol 2010; 60:2398–2408 [View Article][PubMed]
     [Google Scholar]
  13. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013; 30:2725–2729 [View Article][PubMed]
     [Google Scholar]
  14. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981; 17:368–376 [View Article][PubMed]
     [Google Scholar]
  15. Fitch WM. Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 1971; 20:406–416 [View Article]
     [Google Scholar]
  16. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4:406–425[PubMed]
     [Google Scholar]
  17. Kim OS, Cho YJ, Lee K, Yoon SH, Kim M et al. Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 2012; 62:716–721 [View Article][PubMed]
     [Google Scholar]
  18. Marmur J, Doty P. Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 1962; 5:109–118 [View Article][PubMed]
     [Google Scholar]
  19. Kushwaha SC, Kates M, Juez G, Rodriguez-Valera F, Kushner DJ. Polar lipids of an extremely halophilic bacterial strain (R-4) isolated from salt ponds in Spain. Biochim Biophys Acta 1982; 711:19–25 [View Article]
     [Google Scholar]
  20. Kim M, Oh HS, Park SC, Chun J. Towards a taxonomic coherence between average nucleotide identity and 16S rRNA gene sequence similarity for species demarcation of prokaryotes. Int J Syst Evol Microbiol 2014; 64:346–351 [View Article][PubMed]
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.002259
Loading
Halobacterium litoreum sp. nov., isolated from a marine solar saltern
Int J Syst Evol Microbiol 67, 4095 (2017); https://doi.org/10.1099/ijsem.0.002259
/content/journal/ijsem/10.1099/ijsem.0.002259
/content/journal/ijsem/10.1099/ijsem.0.002259
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error