@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.002258, author = "Moh, Tsu Horng and Furusawa, Go and Amirul, Abdullah Al-Ashraf", title = "Microbulbifer aggregans sp. nov., isolated from estuarine sediment from a mangrove forest", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2017", volume = "67", number = "10", pages = "4089-4094", doi = "https://doi.org/10.1099/ijsem.0.002258", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002258", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "cell aggregation", keywords = "Microbulbifer", keywords = "mangrove sediments", abstract = "A novel, rod-shaped, Gram-stain-negative, halophilic and non-motile bacterium, designated CCB-MM1T, was isolated from a sample of estuarine sediment collected from Matang Mangrove Forest, Malaysia. The cells possessed a rod–coccus cell cycle in association with growth phase and formed aggregates. Strain CCB-MM1T was both catalase and oxidase positive, and able to degrade starch. Optimum growth occurred at 30 °C and pH 7.0 in the presence of 2–3 % (w/v) NaCl. The 16S rRNA gene sequence of strain CCB-MM1T showed 98.12, 97.46 and 97.33 % sequence similarity with Microbulbifer rhizosphaerae Cs16bT, Microbulbifer maritimus TF-17T and Microbulbifer gwangyangensis GY2T respectively. Strain CCB-MM1T and M. rhizosphaerae Cs16bT formed a cluster in the phylogenetic tree. The major cellular fatty acids were iso-C17 : 1 ω9c and iso-C15 : 0, and the total polar lipid profile consisted of phosphatidylglycerol, phosphatidylethanolamine, phosphoaminolipid, two unidentified lipids, an unidentified glycolipid and an unidentified aminolipid. The major respiratory quinone was ubiquinone Q-8 and the genomic DNA G+C content of the strain was 58.9 mol%. On the basis of the phylogenetic, phenotypic and genotypic data presented here, strain CCB-MM1T represents a novel species of the genus Microbulbifer , for which the name Microbulbifer aggregans sp. nov. is proposed. The type strain is CCB-MM1T (=LMG 29920T=JCM 31875T).", }