1887

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Volume 67, Issue 10

sp. nov., isolated from a car air-conditioning system Free

Abstract

A Gram-stain-negative, yellow-pigmented bacterial strain, designated PR0302, was isolated from a car evaporator core collected in Korea. The cells were strictly aerobic, non-spore-forming and rod-shaped. The strain grew at 15–37 °C (optimum, 25 °C), at pH 6.0–8.0 (optimum, 7.0) and in the presence of 0–1 % (w/v) NaCl. Phylogenetically, the strain was closely related to members of the genus (97.04–91.22 % 16S rRNA gene sequence similarities) and showed the highest sequence similarity of 97.04 % to THG-DT81. It contained C, summed feature 8 (Cω7 and/or Cω6) and C 2-OH as the predominant fatty acids and Q-10 as the major ubiquinone. The predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine and sphingoglycolipid. The major polyamine was -homospermidine. The serine palmitoyl transferase gene () was detected and sphingolipid synthesis was confirmed. The mean DNA G+C content of the strain was 67.8±0.5 mol%. DNA–DNA relatedness between strain PR0302 and closely related type strains of species was less than 30 %. The low levels of DNA–DNA relatedness identified strain PR0302 as a member of a novel species in the genus . Based on phenotypic, genotypic and chemotaxonomic data, strain PR0302 represents a novel species in the genus , for which the name sp. nov. is proposed. The type strain is PR0302 (=KACC 18487=NBRC 111532).

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  • Accepted:
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Keyword(s): car evaporator core , novel species , polyphasic taxonomy and Sphingomonas carri
© 2017 IUMS
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2017-10-01
2024-04-16
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Sphingomonas carri sp. nov., isolated from a car air-conditioning system
Int J Syst Evol Microbiol 67, 4069 (2017); https://doi.org/10.1099/ijsem.0.002250
/content/journal/ijsem/10.1099/ijsem.0.002250
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