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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 67, Issue 10

sp. nov., isolated from Antarctic soil Free

Abstract

A Gram-stain-negative, aerobic, yellow-pigmented, non-flagellated, non-gliding, rod-like, oxidase- and catalase-positive bacterium, designated A2-1, was isolated from soil on Ardley Island, South Shetland Islands, Antarctica. Strain A2-1 grew at 4–22 °C (optimum, 10 °C), at pH 6.0–8.0 (optimum, pH 6.5) and with 0–1.5 % NaCl (optimum, 0.5 %), but could not produce flexirubin-type pigments. 16S rRNA gene sequence analysis showed that the isolates belonged to the genus . Strain A2-1 had the highest 16S rRNA gene sequence similarity to , and with 95.7, 95.6 and 95.4 %, respectively. The strain A2-1 consisted of a clade with and and simultaneously formed a distinct phyletic lineage in the neighbour-joining phylogenetic tree. Polar lipids of the strain included phosphatidylethanolamine (PE), four unidentified aminolipids and one unidentified lipid. The strain A2-1 contained anteiso-C (20.2 %), iso-C (16.2 %) and C G (11.0 %) as the main fatty acids and the only respiratory quinone was menaquinone MK-6. The genomic DNA G+C content was 34.0 mol%. The polyphasic taxonomic study revealed that the strain A2-1 belongs to a novel species within the genus and the name sp. nov. is proposed. The type strain is A2-1 (=CCTCC AB 2017157=KCTC 52644).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Antarctic soil , Polyphasic taxonomy , sp. nov and The genus Flavobacterium
© 2017 IUMS
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2017-10-01
2024-04-19
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Flavobacterium ardleyense sp. nov., isolated from Antarctic soil
Int J Syst Evol Microbiol 67, 3996 (2017); https://doi.org/10.1099/ijsem.0.002241
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