sp. nov., isolated from lake sediment, and emended description of the genus Free

Abstract

A Gram-stain-negative, non-gliding, aerobic and rod-shaped bacterium, designated XJNY, was isolated from Sayram Lake (44° 30′ 30.41″ N 81° 12′ 39.55″ E), Xinjiang Province, north-west China, and was characterized taxonomically by a polyphasic study. Strain XJNY grew at salinities of 0–4 % (w/v) and temperatures of 4–37 °C. The pH range for growth was 6.5–8.5. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain XJNY belonged to the genus and was closely related to the type strain of with 97.8 % similarity. The DNA G+C content was 34.5 mol%. The major fatty acids were iso-C, iso-C G, anteiso-C, iso-C 3-OH, C 2-OH and iso-C 3-OH. The major respiratory isoprenoid quinone was menaquinone-6 (MK-6) and the polar lipids were phosphatidylethanolamine, an unidentified phospholipid, two unidentified aminolipids and four unidentified lipids. On the basis of morphological, physiological and molecular properties and phylogenetic distinctiveness, strain XJNY represents a novel species within the genus , for which the name sp. nov. is proposed. The type strain is XJNY (=KCTC 52638=MCCC 1H00183).

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2017-10-01
2024-03-28
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References

  1. Kim S, Jung YT, Park JM, Yoon JH, Park S. Confluentibacter lentus gen. nov., sp. nov., isolated from the junction between the ocean and a freshwater lake. Int J Syst Evol Microbiol 2016; 66:868–873 [Crossref]
    [Google Scholar]
  2. Liu QQ, Wang Y, Li J, Du ZJ, Chen GJ. Saccharicrinis carchari sp. nov., isolated from a shark, and emended descriptions of the genus Saccharicrinis and Saccharicrinis fermentans. Int J Syst Evol Microbiol 2014; 64:2204–2209 [View Article][PubMed]
    [Google Scholar]
  3. Mesbah M, Premachandran U, Whitman WB. Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 1989; 39:159–167 [View Article]
    [Google Scholar]
  4. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 2016; 33:1870–1874 [View Article][PubMed]
    [Google Scholar]
  5. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985; 39:783791 [View Article][PubMed]
    [Google Scholar]
  6. Kim M, Oh HS, Park SC, Chun J. Towards a taxonomic coherence between average nucleotide identity and 16S rRNA gene sequence similarity for species demarcation of prokaryotes. Int J Syst Evol Microbiol 2014; 64:346–351 [View Article][PubMed]
    [Google Scholar]
  7. Richter M, Rosselló-Móra R. Shifting the genomic gold standard for the prokaryotic species definition. Proc Natl Acad Sci USA 2009; 106:19126–19131 [View Article][PubMed]
    [Google Scholar]
  8. Bowman JP. Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov. Int J Syst Evol Microbiol 2000; 50:1861–1868 [View Article][PubMed]
    [Google Scholar]
  9. Dong XZ, Cai MY. (editors) Chapter 14. Determination of biochemical characteristics. Manual for the Systematic Identification of General Bacteria Beijing: Science Press (in Chinese); 2001 pp. 370–398
    [Google Scholar]
  10. Bernardet JF, Nakagawa Y, Holmes B. Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 2002; 52:1049–1070 [View Article][PubMed]
    [Google Scholar]
  11. CLSI Performance Standards for Antimicrobial Susceptibility Testing; Twenty-Second Informational Supplement Wayne, PA: Clinical and Laboratory Standards Institute; 2012
    [Google Scholar]
  12. Tindall BJ. Lipid composition of Halobacterium lacusprofundi . FEMS Microbiol Lett 1990; 66:199–202 [View Article]
    [Google Scholar]
  13. Tindall BJ. A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. Syst Appl Microbiol 1990
    [Google Scholar]
  14. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101. Newark, DE: MIDI Inc; 1990
    [Google Scholar]
  15. Bligh EG, Dyer WJ. A rapid method of total lipid extraction and purification. Can J Biochem Physiol 1959; 37:911–917 [View Article][PubMed]
    [Google Scholar]
  16. Tindall BJ, Sikorski J, Smibert RM, Kreig NR. Phenotypic characterization and the principles of comparative systematics. In Reddy CA, Beveridge TJ, Breznak JA, Marzluf G, Schmidt TM. et al. (editors) Methods for General and Molecular Microbiology, 3rd ed. Washington, DC: ASM Press; 2007 pp. 330–393
    [Google Scholar]
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