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Abstract

A yellow-pigmented strain, designated Y4AR-5, was characterized by using a polyphasic approach. The strain was isolated from a tundra soil from near Longyearbyen, Svalbard Islands, Norway. The cells were Gram-stain-negative, aerobic, rod-shaped and non-motile. Growth occurred at 4–28 °C (optimum 20 °C) and pH 6.0–9.0 (optimum pH 8.0) and with 0–0.5 % (w/v) NaCl (optimum 0 %). The major respiratory quinone was MK-7. The polar lipids were phosphatidylethanolamine (PE), an aminophospholipid (APL), a phospholipid (PL), an unidentified aminolipid (AL) and two unidentified lipids. The results of analysis of the 16S rRNA gene indicated that the novel strain was most closely related to members of the genus Spirosoma (96.2 % sequence similarity with Spirosoma endophyticum ). The genomic DNA G+C content was 45.9 mol%. The major cellular fatty acids were summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), C16 : 1ω5c, iso-C17 : 0 3-OH and iso-C15 : 0. On the basis of its phenotypic and genotypic properties, strain Y4AR-5 should be classified as representing a novel species of the genus Spirosoma , for which the name Spirosoma flavum sp. nov. is proposed. The type strain is Y4AR-5 (=CCTCC AB 2015352=KCTC 52490).

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2017-10-23
2019-10-20
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