RT Journal Article SR Electronic(1) A1 Sun, Cong A1 Wu, Chen A1 Su, Yue A1 Wang, Rui-jun A1 Fu, Ge-yi A1 Zhao, Zhe A1 Yu, Xiao-yun A1 Huang, Meng-meng A1 Han, Bing-nan A1 Lv, Zheng-bing A1 Wu, MinYR 2017 T1 Hyphococcus flavus gen. nov., sp. nov., a novel alphaproteobacterium isolated from deep seawater JF International Journal of Systematic and Evolutionary Microbiology, VO 67 IS 10 SP 4024 OP 4031 DO https://doi.org/10.1099/ijsem.0.002237 PB Microbiology Society, SN 1466-5034, AB A Gram-staining-negative, aerobic, non-spore-forming, coccoid to rod shaped bacteria with prosthecate and flagellum, designated as HSF6T, was isolated from deep seawater samples collected from the South China Sea at depth of 2.5 km and subjected to a polyphasic taxonomic investigation. Colonies of strain HSF6T were 1–2 mm in diameter, smooth, circular, convex and yellow. Strain HSF6T was found to grow at 15–37 °C (optimum, 25–35 °C), pH 5.0–9.5 (optimum, pH 7.0–7.5) and with 0–8 % (w/v) NaCl (optimum, 2 %). Chemotaxonomic analysis showed the predominant respiratory quinone of strains HSF6T were ubiquinone-10, and the major fatty acids were C18 : 1ω7c, C16 : 0 and 11-methyl C18 : 1 ω7c. The polar lipids were monoglycosyldiglyceride (MGDG), sulfo-quinovosyl diacylglycerol (SQDG), three unknown glycolipids (GL1–3) and five unknown lipids (L1–5). The DNA G+C content of strain HSF6T was determined to be 51.0 mol% with HPLC. The comparison of 16S rRNA gene sequence similarities show that strain HSF6T was related most closely to genus Parvularcula with similarity ranging from 91.0 to 91.8 %. The phylogenetic trees, using the 16S rRNA gene sequence, reconstructed with neighbour-joining, maximum-parsimony and maximum-likelihood methods showed that strain HSF6T constituted a separated branch in the family ‘ Parvularculaceae ’. Differential phenotypic properties, together with the phylogenetic distinctiveness, demonstrated that strain HSF6T is clearly distinct from validly published genera. On the basis of these features, we propose strain HSF6T (=MCCC 1K03223T=KCTC 52486T) represents a novel species of a novel genus with the name Hyphococcus flavus gen. nov., sp. nov., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002237