1887

Abstract

A novel, Gram-stain-positive, moderately halophilic, endospore-forming, motile, facultatively anaerobic and rod-shaped strain, designated 0W14, was isolated from a marine saltern of Wendeng, China. Optimal growth occurred at 37 °C, pH 7.5 and with 6.0 % (w/v) NaCl. MK-7 was the sole respiratory quinone and the peptidoglycan type of 0W14 was A4β l-Orn–d-Glu. The major cellular fatty acid (>10.0 %) in strain 0W14 was anteiso-C15 : 0. The polar lipid profile of strain 0W14 consisted of diphosphatidylglycerol, phosphatidylglycerol, two unknown glycolipids and four unknown phospholipids. The genomic DNA G+C content of the strain was 44.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain 0W14 forms a phylogenetic lineage with members of the genus Lentibacillus within the family Bacillaceae . Based on data from the current polyphasic study, the isolate is proposed to represent a novel species of genus Lentibacillus , for which the name Lentibacillus sediminis sp. nov. is proposed. The type strain is 0W14 (=KCTC 33835=MCCC 1H00171).

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2017-09-12
2019-12-05
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References

  1. Kushner D, Kamekura M. Physiology of halophilic eubacteria. In Rodriguez-Valera F. (editor) Halophilic Bacteria Boca Raton, FL: CRC Press; 1988; pp. 109– 140
    [Google Scholar]
  2. Yoon JH, Kang KH, Park YH. Lentibacillus salicampi gen. nov., sp. nov., a moderately halophilic bacterium isolated from a salt field in Korea. Int J Syst Evol Microbiol 2002; 52: 2043– 2048 [CrossRef] [PubMed]
    [Google Scholar]
  3. Tanasupawat S, Pakdeeto A, Namwong S, Thawai C, Kudo T et al. Lentibacillus halophilus sp. nov., from fish sauce in Thailand. Int J Syst Evol Microbiol 2006; 56: 1859– 1863 [CrossRef] [PubMed]
    [Google Scholar]
  4. Oh YJ, Lee HW, Lim SK, Kwon MS, Lee J et al. Lentibacillus kimchii sp. nov., an extremely halophilic bacterium isolated from kimchi, a Korean fermented vegetable. Antonie van Leeuwenhoek 2016; 109: 869– 876 [CrossRef] [PubMed]
    [Google Scholar]
  5. Lane DJ. 16S/23S rRNA sequencing. In Goodfellow M, Stackebrandt E. (editors) Nucleic Acid Techniques in Bacterial Systematics Chichester: Wiley; 1991; pp. 115– 147
    [Google Scholar]
  6. Marchesi JR, Sato T, Weightman AJ, Martin TA, Fry JC et al. Design and evaluation of useful bacterium-specific PCR primers that amplify genes coding for bacterial 16S rRNA. Appl Environ Microbiol 1998; 64: 795– 799 [PubMed]
    [Google Scholar]
  7. Yoon SH, Ha SM, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017; 67: 1613– 1617 [CrossRef] [PubMed]
    [Google Scholar]
  8. Pruesse E, Peplies J, Glöckner FO. SINA: accurate high-throughput multiple sequence alignment of ribosomal RNA genes. Bioinformatics 2012; 28: 1823– 1829 [CrossRef] [PubMed]
    [Google Scholar]
  9. Yarza P, Richter M, Peplies J, Euzeby J, Amann R et al. The All-Species Living Tree project: a 16S rRNA-based phylogenetic tree of all sequenced type strains. Syst Appl Microbiol 2008; 31: 241– 250 [CrossRef] [PubMed]
    [Google Scholar]
  10. Westram R, Bader K, Pruesse E, Kumar Y, Meier H et al. ARB: a software environment for sequence data. In Bruijn FJD. (editor) Handbook of Molecular Microbial Ecology I: Metagenomics and Complementary Approaches Hoboken, NJ: Wiley; 2011; pp. 399– 406 [Crossref]
    [Google Scholar]
  11. Jukes TH, Cantor CR. Evolution of protein molecules. In Munro HN. (editor) Mammalian Protein Metabolism New York, NY: Academic Press; 1969; pp. 21– 132 [Crossref]
    [Google Scholar]
  12. Stamatakis A. RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics 2014; 30: 1312– 1313 [CrossRef] [PubMed]
    [Google Scholar]
  13. Felsenstein J. Phylip (Phylogeny Inference Package) Version 3.6 , Distributed by the author. Seattle, USA: Department of Genome Sciences, University of Washington; 2005
    [Google Scholar]
  14. Chen YG, Liu ZX, Peng DJ, Zhang YQ, Wang YX et al. Virgibacillus litoralis sp. nov., a moderately halophilic bacterium isolated from saline soil. Antonie van Leeuwenhoek 2009; 96: 323– 329 [CrossRef] [PubMed]
    [Google Scholar]
  15. Heyndrickx M, Lebbe L, Kersters K, Hoste B, De Wachter R et al. Proposal of Virgibacillus proomii sp. nov. and emended description of Virgibacillus pantothenticus (Proom and Knight 1950) Heyndrickx et al. 1998. Int J Syst Bacteriol 1999; 49: 1083– 1090 [CrossRef] [PubMed]
    [Google Scholar]
  16. Lu J, Nogi Y, Takami H. Oceanobacillus iheyensis gen. nov., sp. nov., a deep-sea extremely halotolerant and alkaliphilic species isolated from a depth of 1050 m on the Iheya Ridge. FEMS Microbiol Lett 2001; 205: 291– 297 [CrossRef] [PubMed]
    [Google Scholar]
  17. Richter M, Rosselló-Móra R. Shifting the genomic gold standard for the prokaryotic species definition. Proc Natl Acad Sci USA 2009; 106: 19126– 19131 [CrossRef] [PubMed]
    [Google Scholar]
  18. Qin QL, Xie BB, Zhang XY, Chen XL, Zhou BC et al. A proposed genus boundary for the prokaryotes based on genomic insights. J Bacteriol 2014; 196: 2210– 2215 [CrossRef] [PubMed]
    [Google Scholar]
  19. Bernardet JF, Nakagawa Y, Holmes B. Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 2002; 52: 1049– 1070 [CrossRef] [PubMed]
    [Google Scholar]
  20. Smibert RM, Krieg NR. Phenotypic characterization. In Gerhardt P, Murray RGE, Wood WA, Krieg NR. (editors) Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology; 1994; pp. 607– 654
    [Google Scholar]
  21. Cowan ST, Steel KJ. Bacterial Characters and Characterization, 2nd ed. Cambridge, UK: Cambridge University Press; 1974
    [Google Scholar]
  22. Dong XZ, Cai MY. Determination of biochemical characteristics. In Dong XZ, Cai MY. (editors) Manual for the Systematic Identification of General Bacteria Beijing: Science Press; 2001; pp. 370– 398
    [Google Scholar]
  23. Hiraishi A, Ueda Y, Ishihara J, Mori T. Comparative lipoquinone analysis of influent sewage and activated sludge by high-performance liquid chromatography and photodiode array detection. J Gen Appl Microbiol 1996; 42: 457– 469 [CrossRef]
    [Google Scholar]
  24. Tindall BJ, Sikorski J, Smibert RA, Krieg NR. Phenotypic characterization and the principles of comparative systematics. In Reddy CA, Beveridge TJ, Breznak JA, Marzluf GA, Schmidt TM. et al. (editors) Methods for General and Molecular Microbiology, 3rd ed. Washington, DC: American Society for Microbiology; 2007; pp. 330– 393
    [Google Scholar]
  25. Schumann P. Peptidoglycan structure. Methods Microbiol 2011; 38: 101– 129 [Crossref]
    [Google Scholar]
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