1887

Abstract

Three bacterial isolates from human faeces, YS-aM39, R2F3-3-3 and R2F3-5-1, were characterized as Gram-negative, strictly anaerobic, non-spore-forming, non-motile, and rod-shaped. Isolate YS-aM39 formed a distinct line of descent, showing greatest 16S rRNA gene sequence relatedness with R2F3-3-3 (97.5 %), R2F3-5-1 (97.5 %), Bacteroides ovatus (98.8 %) and Bacteroides xylanisolvens (97.2 %). Isolates R2F3-3-3 and R2F3-5-1 also formed a distinct line of descent, sharing greatest 16S rRNA gene sequence relatedness with B. ovatus (98.2 %) and B. xylanisolvens (97.2 %). The DNA G+C content of YS-aM39 was 44.8 mol%, that of R2F3-3-3 was 42.4 mol% and that of R2F3-5-1 was 42.6 mol%. The respiratory quinone of all three isolates was menaquinone MK-10. Polar lipid analysis identified phosphatidylethanolamine as the major lipid. The predominant fatty acids in all three isolates were anteiso-C15 : 0, iso-C15 : 0, C16 : 0 3-OH and iso-C17 : 0 3-OH. The major end products of glucose fermentation were acetic acid, lactic acid and formic acid. DNA–DNA hybridization data indicated that two isolates, YS-aM39 and R2F3-3-3, represent a species distinct from B. ovatus and B. xylanisolvens . Finally, in this study, the two isolates represented two new species in the genus Bacteroides , for which we propose the names Bacteroides koreensis sp. nov. (type strain, YS-aM39=KCTC 15520=JCM 31393) and Bacteroides kribbi sp. nov. (type strain, R2F3-3-3=KCTC 15460=JCM 31391).

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2017-09-25
2019-10-23
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