1887

Abstract

A Gram-stain-positive, catalase-negative and short-rod-shaped organism, designated VTT E-94560, was isolated from beer in Finland and deposited in the VTT culture collection as a strain of . However, the results of 16S rRNA gene sequence analysis showed that VTT E-94560 was only related to JCM 16176 with 97.0 % sequence similarity, lower than the 98.7 % regarded as the boundary for the species differentiation. Additional phylogenetic studies on the gene, gene and 16S–23S rRNA internally transcribed spacer region further reinforced the taxonomically independent status of VTT E-94560 and its related species including and . Strain VTT E-94560 also exhibited several differences in its carbohydrate fermentation profiles from those related species. In addition, DNA–DNA relatedness between VTT E-94560 and these two type strains was 4 % ( JCM 16176) and 12 % ( JCM 16155), respectively, which were lower than the 70 % cut-off for general species delineation, indicating that these three strains are not taxonomically identical at the species level. These studies revealed that VTT E-94560 represents a novel species, for which the name sp. nov. is proposed. The type strain is VTT E-94560 (=JCM 31185).

Keyword(s): beer and Lactobacillus cultus
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2017-10-01
2020-01-27
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