1887

Abstract

A novel Gram-stain-negative, strictly aerobic, rod-shaped and agar-hydrolysing bacterium, designated D2, was isolated from a marine sediment sample collected from the coast of Weihai, China (37° 31′ 59″ N 122° 03′ 47″ E). The cells were motile by a lateral flagellum. Growth was observed at 10–42 °C, at pH 6.0–9.0 and with 0.5–8 % (w/v) NaCl. Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain D2 belonged to the genus , appearing closely related to YM01 (96.3 % 16S rRNA gene sequence similarity) and Q1 (93.9 %). The dominant fatty acids were summed feature 3 (Cω7 and/or iso-C 2-OH), C and C 3-OH. The polar lipids were phosphatidylethanolamine, phosphatidylglycerol, two phosphoaminolipids, two unknown lipids and three phospholipids. Ubiquinone 8 (Q-8) was found to be the major respiratory quinone. The DNA G+C content was 40.4 mol%. On the basis of genotypic, phenotypic and phylogenetic evidence, strain D2 is presented as a representative of a novel species of the genus , for which the name sp. nov. is proposed. The type strain is D2 (=KCTC 42869=MCCC 1H00129). An emended description of the genus is also provided.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.002219
2017-10-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/67/10/3894.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.002219&mimeType=html&fmt=ahah

References

  1. Yan S, Yu M, Wang Y, Shen C, Zhang XH. Catenovulum agarivorans gen. nov., sp. nov., a peritrichously flagellated, chain-forming, agar-hydrolysing gammaproteobacterium from seawater. Int J Syst Evol Microbiol 2011; 61:2866–2873 [View Article][PubMed]
    [Google Scholar]
  2. Li DQ, Zhou YX, Liu T, Chen GJ, Du ZJ. Catenovulum maritimus sp. nov., a novel agarolytic gammaproteobacterium isolated from the marine alga Porphyra yezoensis Ueda (AST58-103), and emended description of the genus Catenovulum. Antonie van Leeuwenhoek 2015; 108:427–434 [View Article][PubMed]
    [Google Scholar]
  3. Liu QQ, Wang Y, Li J, Du ZJ, Chen GJ. Saccharicrinis carchari sp. nov., isolated from a shark, and emended descriptions of the genus Saccharicrinis and Saccharicrinis fermentans. Int J Syst Evol Microbiol 2014; 64:2204–2209 [View Article][PubMed]
    [Google Scholar]
  4. Kim OS, Cho YJ, Lee K, Yoon SH, Kim M et al. Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 2012; 62:716–721 [View Article][PubMed]
    [Google Scholar]
  5. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 1997; 25:4876–4882 [View Article][PubMed]
    [Google Scholar]
  6. Hall TA. BioEdit: a user-friendly sequence alignment editor and analysis program for windows 95/98/NT. Nucl Acids Symp Ser 1999; 41:95–98
    [Google Scholar]
  7. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 2016; 33:1870–1874 [View Article][PubMed]
    [Google Scholar]
  8. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980; 16:111–120 [View Article][PubMed]
    [Google Scholar]
  9. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985; 39:783–791 [View Article][PubMed]
    [Google Scholar]
  10. Smibert RM, Krieg NR. Phenotypic characterization. In Gerhardt P, Murray RGE, Wood WA, Krieg NR. (editors) Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology; 1994 pp. 607–654
    [Google Scholar]
  11. Bowman JP. Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov. Int J Syst Evol Microbiol 2000; 50:1861–1868 [View Article][PubMed]
    [Google Scholar]
  12. Xia HF, Li XL, Liu QQ, Miao TT, Du ZJ et al. Salegentibacter echinorum sp. nov., isolated from the sea urchin Hemicentrotus pulcherrimus. Antonie van Leeuwenhoek 2013; 104:315–320 [View Article][PubMed]
    [Google Scholar]
  13. Dong XZ, Cai MY. Determination of biochemical characteristics. In Manual for the Systematic Identification of General Bacteria Beijing, China: Science Press; 2001 pp. 370–398
    [Google Scholar]
  14. CLSI Performance Standards for Antimicrobial Susceptibility Testing 22nd Informational Supplement M100-S22 Wayne, PA: Clinical and Laboratory Standards Institute; 2012
    [Google Scholar]
  15. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101. Newark, DE: MIDI Inc; 1990
    [Google Scholar]
  16. Kroppenstedt RM. Separation of bacterial menaquinones by HPLC using reverse phase (RP18) and a silver loaded Ion exchanger as stationary phases. J Liq Chromatogr 1982; 5:2359–2367 [View Article]
    [Google Scholar]
  17. Tindall BJ, Sikorski J, Smibert RM, Kreig NR. Phenotypic characterization and the principles of comparative systematics. In Reddy CA, Beveridge TJ, Breznak JA, Marzluf G, Schmidt TM. et al (editors) Methods for General and Molecular Microbiology, 3rd ed. Washington, DC: ASM Press; 2007 pp. 330–393
    [Google Scholar]
  18. Mesbah M, Premachandran U, Whitman WB. Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 1989; 39:159–167 [View Article]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.002219
Loading
/content/journal/ijsem/10.1099/ijsem.0.002219
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error