1887

Abstract

A previously undescribed, rapidly growing, scotochromogenic species of the genus (represented by strains PB739 and GK) was isolated from two clinical sources – the sputum of a 76-year-old patient with severe chronic obstructive pulmonary disease, history of tuberculosis exposure and complex isolated years prior; and the blood of a 15-year-old male with B-cell acute lymphoblastic leukaemia status post bone marrow transplant. The isolates grew as dark orange colonies at 25–37 °C after 5 days, sharing features in common with other closely related species. Analysis of the complete 16S rRNA gene sequence (1492 bp) of strain PB739 demonstrated that the isolate shared 98.8 % relatedness with . Partial 429 bp and 744 bp region V sequence analyses revealed that the sequences of the novel isolate shared 94.8 and 92.1 % similarity with those of and respectively. Biochemical profiling, antimicrobial susceptibility testing, HPLC/gas-liquid chromatography analyses and multilocus sequence typing support the taxonomic status of these isolates (PB739 and GK) as representatives of a novel species. Both isolates were susceptible to the Clinical and Laboratory Standards Institute recommended antimicrobials for susceptibility testing of rapidly growing mycobacteria including amikacin, ciprofloxacin, moxifloxacin, doxycycline/minocycline, imipenem, linezolid, clarithromycin and trimethropin/sulfamethoxazole. Both isolates PB739 and GK showed intermediate susceptibility to cefoxitin. We propose the name sp. nov. for this novel species and have deposited the type strain in the DSMZ and CIP culture collections. The type strain is PB739 (=DSM 104744=CIP 111318).

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2017-11-01
2024-12-13
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