1887

Abstract

The diversity of species discovered in California appears to be particularly high. A divergent group of strains collected from ticks in California was described by Postic and co-workers and designated ‘genomospecies 2’ (Postic D, Garnier M, Baranton G. 2007;297:263–271; Postic D, Ras NM, Lane RS, Hendson M, Baranton G. 1998;36:3497–3504). We performed multilocus sequence analysis (MLSA) using eight housekeeping loci (, , , , , , and ) on 12 strains of this genospecies to confirm that these strains form a distinct group within the s. l. complex (Margos G, Hojgaard A, Lane RS, Cornet M, Fingerle V 2010;1:151–158). Phylogenetic and genetic distance analyses based on sequences of the MLSA housekeeping genes corroborated the distinctness of this group; genetic distances to all other members of the s.l. complex were 96 % or lower. We propose the name sp. nov. for this genospecies in honor of Professor Robert S. Lane, University of California Berkeley, for his contributions to and tick research. The type strain for sp. nov., strain CA28-91, has been deposited to two culture collections (=DSM 17992=CIP 109135).

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2017-10-01
2020-10-27
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References

  1. Kurtenbach K, Hanincová K, Tsao JI, Margos G, Fish D et al. Fundamental processes in the evolutionary ecology of Lyme borreliosis. Nat Rev Microbiol 2006;4:660–669 [CrossRef][PubMed]
    [Google Scholar]
  2. Brown RN, Lane RS. Lyme disease in California: a novel enzootic transmission cycle of Borrelia burgdorferi. Science 1992;256:1439–1442 [CrossRef][PubMed]
    [Google Scholar]
  3. Brown RN, Lane RS. Reservoir competence of four chaparral-dwelling rodents for Borrelia burgdorferi in California. Am J Trop Med Hyg 1996;54:84–91 [CrossRef][PubMed]
    [Google Scholar]
  4. Schwan TG, Schrumpf ME, Karstens RH, Clover JR, Wong J et al. Distribution and molecular analysis of lyme disease spirochetes, Borrelia burgdorferi, isolated from ticks throughout California. J Clin Microbiol 1993;31:3096–3108[PubMed]
    [Google Scholar]
  5. Postic D, Garnier M, Baranton G. Multilocus sequence analysis of atypical Borrelia burgdorferi sensu lato isolates-description of Borrelia californiensis sp. nov., and genomospecies 1 and 2. Int J Med Microbiol 2007;297:263–271 [CrossRef][PubMed]
    [Google Scholar]
  6. Postic D, Ras NM, Lane RS, Hendson M, Baranton G. Expanded diversity among Californian Borrelia isolates and description of Borrelia bissettii sp. nov. (formerly Borrelia group DN127). J Clin Microbiol 1998;36:3497–3504[PubMed]
    [Google Scholar]
  7. Margos G, Vollmer SA, Cornet M, Garnier M, Fingerle V et al. A new Borrelia species defined by multilocus sequence analysis of housekeeping genes. Appl Environ Microbiol 2009;75:5410–5416 [CrossRef][PubMed]
    [Google Scholar]
  8. Tamura K, Peterson D, Peterson N, Stecher G, Nei M et al. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 2011;28:2731–2739 [CrossRef][PubMed]
    [Google Scholar]
  9. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980;16:111–120 [CrossRef][PubMed]
    [Google Scholar]
  10. Nei M, Kumar S. Molecular Evolution and Phylogenetics New York: Oxford University Press; 2000
    [Google Scholar]
  11. Kingry LC, Batra D, Replogle A, Rowe LA, Pritt BS et al. Whole genome sequence and comparative genomics of the novel Lyme Borreliosis causing pathogen, Borrelia mayonii. PLoS One 2016;11:e0168994 [CrossRef][PubMed]
    [Google Scholar]
  12. Pritt BS, Respicio-Kingry LB, Sloan LM, Schriefer ME, Replogle AJ et al. Borrelia mayonii sp. nov., a member of the Borrelia burgdorferi sensu lato complex, detected in patients and ticks in the upper midwestern United States. Int J Syst Evol Microbiol 2016;66:4878–4880 [CrossRef][PubMed]
    [Google Scholar]
  13. Fedorova N, Kleinjan JE, James D, Hui LT, Peeters H et al. Remarkable diversity of tick or mammalian-associated Borreliae in the metropolitan San Francisco Bay area, California. Ticks Tick Borne Dis 2014;5:951–961 [CrossRef][PubMed]
    [Google Scholar]
  14. Girard YA, Travinsky B, Schotthoefer A, Fedorova N, Eisen RJ et al. Population structure of the Lyme Borreliosis spirochete Borrelia burgdorferi in the western black-legged tick (Ixodes pacificus) in Northern California. Appl Environ Microbiol 2009;75:7243–7252 [CrossRef][PubMed]
    [Google Scholar]
  15. Scott JD, Clark KL, Foley JE, Anderson JF, Durden LA et al. Detection of Borrelia genomospecies 2 in Ixodes spinipalpis ticks collected from a rabbit in Canada. J Parasitol 2017;103:38–46 [CrossRef][PubMed]
    [Google Scholar]
  16. Johnson RC, Schmid GP, Hyde FW, Steigerwalt AG, Brenner DJ. Borrelia burgdorferi sp. nov.: etiologic agent of Lyme disease. Int J Syst Bacteriol 1984;34:496–497 [CrossRef]
    [Google Scholar]
  17. Margos G, Hojgaard A, Lane RS, Cornet M, Fingerle V et al. Multilocus sequence analysis of Borrelia bissettii strains from North America reveals a new Borrelia species, Borrelia kurtenbachii. Ticks Tick Borne Dis 2010;1:151–158 [CrossRef][PubMed]
    [Google Scholar]
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