@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.002209, author = "Morandi, Stefano and Cremonesi, Paola and Povolo, Milena and Capra, Emanuele and Silvetti, Tiziana and Castiglioni, Bianca and Ribeiro, Márcio Garcia and Alves, Ana Carolina and da Costa, Geraldo Márcio and Luini, Mario and Brasca, Milena", title = "Prototheca blaschkeae subsp. brasiliensis subsp. nov., isolated from cow milk", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2017", volume = "67", number = "10", pages = "3865-3871", doi = "https://doi.org/10.1099/ijsem.0.002209", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002209", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "cow milk", keywords = "Prototheca blaschkeae subsp. brasiliensis", keywords = "protothecal bovine mastitis", abstract = "A strain of an achlorophyllic alga, named PR24T, was isolated from cow milk samples from the state of Minas Gerais, Brazil. Based on 18S rDNA, 28S rRNA, D1/D2 region of the LSU rDNA and SSU rRNA gene sequence similarities, this strain was found to be a member of the genus Prototheca and closely related to Prototheca blaschkeae SAG2064T. However, the novel strain could easily be distinguished from recognized Prototheca species by internal transcribed spacer, species-specific PCR, single-strand conformation polymorphism-PCR analysis and phenotypic characteristics. The inability to grow in Sabouraud broth at pH 4.0 and the different cellular fatty acid composition clearly distinguished PR24T from the reference strain of P. blaschkeae. The combination of genotypic and phenotypic data indicates that strain PR24T represents a subspecies of P. blaschkeae, for which the name Prototheca blaschkeae subsp. brasiliensis subsp. nov. is proposed. The respective type strain is PR24T (=DSM 103592T=IHEM 26958T).", }