1887

Abstract

A novel bacterium, designated HME9613, was isolated from seawater of the Yellow Sea in the Republic of Korea. Cells of strain HME9613 were Gram-stain-negative, aerobic, motile by gliding and rod-shaped. Strain HME9613 grew optimally at 25 °C, at pH 7 and in the presence of 3 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain HME9613 formed a lineage within the genus Winogradskyella of the family Flavobacteriaceae , and showed highest sequence similarity to Winogardskyella exilis 022-2-26 (95.9 %). The 16S rRNA gene sequence similarity between strain HME9613 and other Winogradskyella species ranged from 93.7 to 95.9 %. The major fatty acids were iso-C15 : 1 G, summed feature 3 (C16 : 1 ω7c and/or C16 : 1 ω6c) and iso-C15 : 0. The polar lipid profile consisted of phosphatidylethanolamine, two unidentified aminolipids and two unidentified lipids. The predominant respiratory quinone was menaquinone-6 (MK-6). The DNA G+C content of the strain HME9613 was 38.4 mol%. On the basis of phenotypic, chemotaxonomic, phylogenetic and genotypic data, strain HME9613 represents a novel species within the genus Winogradskyella , for which the name Winogradskyella maritima sp. nov. is proposed. The type strain is HME9613 (=KCTC 42189=CECT 8979).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.002205
2017-09-08
2019-12-05
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/67/10/3840.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.002205&mimeType=html&fmt=ahah

References

  1. Nedashkovskaya OI, Kim SB, Han SK, Snauwaert C, Vancanneyt M et al. Winogradskyella thalassocola gen. nov., sp. nov., Winogradskyella epiphytica sp. nov. and Winogradskyella eximia sp. nov., marine bacteria of the family Flavobacteriaceae. Int J Syst Evol Microbiol 2005; 55: 49– 55 [CrossRef] [PubMed]
    [Google Scholar]
  2. Ivanova EP, Christen R, Gorshkova NM, Zhukova NV, Kurilenko VV et al. Winogradskyella exilis sp. nov., isolated from the starfish Stellaster equestris, and emended description of the genus Winogradskyella. Int J Syst Evol Microbiol 2010; 60: 1577– 1580 [CrossRef] [PubMed]
    [Google Scholar]
  3. Yoon BJ, Byun HD, Kim JY, Lee DH, Kahng HY et al. Winogradskyella lutea sp. nov., isolated from seawater, and emended description of the genus Winogradskyella. Int J Syst Evol Microbiol 2011; 61: 1539– 1543 [CrossRef] [PubMed]
    [Google Scholar]
  4. Nedashkovskaya OI, Kukhlevskiy AD, Zhukova NV. Winogradskyella ulvae sp. nov., an epiphyte of a Pacific seaweed, and emended descriptions of the genus Winogradskyella and Winogradskyella thalassocola, Winogradskyella echinorum, Winogradskyella exilis and Winogradskyella eximia. Int J Syst Evol Microbiol 2012; 62: 1450– 1456 [CrossRef] [PubMed]
    [Google Scholar]
  5. Begum Z, Srinivas TN, Manasa P, Sailaja B, Sunil B et al. Winogradskyella psychrotolerans sp. nov., a marine bacterium of the family Flavobacteriaceae isolated from Arctic sediment. Int J Syst Evol Microbiol 2013; 63: 1646– 1652 [CrossRef] [PubMed]
    [Google Scholar]
  6. Park S, Park JM, Won SM, Yoon JH. Winogradskyella crassostreae sp. nov., isolated from an oyster (Crassostrea gigas). Int J Syst Evol Microbiol 2015; 65: 2890– 2895 [CrossRef] [PubMed]
    [Google Scholar]
  7. Kim JY, Park SH, Seo GY, Kim YJ, Oh DC. Winogradskyella eckloniae sp. nov., a marine bacterium isolated from the brown alga Ecklonia cava. Int J Syst Evol Microbiol 2015; 65: 2791– 2796 [CrossRef] [PubMed]
    [Google Scholar]
  8. Nedashkovskaya OI, Kukhlevskiy AD, Zhukova NV, Kim SJ, Rhee SK et al. Winogradskyella litoriviva sp. nov., isolated from coastal seawater. Int J Syst Evol Microbiol 2015; 65: 3652– 3657 [CrossRef] [PubMed]
    [Google Scholar]
  9. Zhang DC, Liu YX, Huang HJ, Weber K, Margesin R. Winogradskyella sediminis sp. nov., isolated from marine sediment. Int J Syst Evol Microbiol 2016; 66: 3157– 3163 [CrossRef] [PubMed]
    [Google Scholar]
  10. Lane DJ. 16S/23S rRNA sequencing. In Stackebrandt E, Goodfellow M. (editors) Nucleic Acid Techniques in Bacterial Systematics Chichester: Wiley; 1991; pp. 125– 175
    [Google Scholar]
  11. Yoon SH, Ha SM, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017; 67: 1613– 1617 [CrossRef] [PubMed]
    [Google Scholar]
  12. Pruesse E, Peplies J, Glöckner FO. SINA: accurate high-throughput multiple sequence alignment of ribosomal RNA genes. Bioinformatics 2012; 28: 1823– 1829 [CrossRef] [PubMed]
    [Google Scholar]
  13. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 2016; 33: 1870– 1874 [CrossRef] [PubMed]
    [Google Scholar]
  14. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4: 406– 425 [PubMed]
    [Google Scholar]
  15. Fitch WM. Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 1971; 20: 406– 416 [CrossRef]
    [Google Scholar]
  16. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981; 17: 368– 376 [CrossRef] [PubMed]
    [Google Scholar]
  17. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985; 39: 783– 791 [CrossRef] [PubMed]
    [Google Scholar]
  18. Stackebrandt E, Goebel BM. Taxonomic note: a place for DNA–DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Evol Microbiol 1994; 44: 846– 849 [CrossRef]
    [Google Scholar]
  19. Gonzalez JM, Saiz-Jimenez C. A fluorimetric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperature. Environ Microbiol 2002; 4: 770– 773 [PubMed] [Crossref]
    [Google Scholar]
  20. Joung Y, Lee BI, Kang H, Kim H, Joh K. Chitinimonas viridis sp. nov., isolated from a mesotrophic artificial lake. Int J Syst Evol Microbiol 2014; 64: 1123– 1126 [CrossRef] [PubMed]
    [Google Scholar]
  21. Brown AE. Benson’s Microbiological Application Laboratory Manual in General Microbiology, 10th ed. New York: McGraw-Hill; 2007
    [Google Scholar]
  22. Hucker GJ. A New Modification and application of the gram stain. J Bacteriol 1921; 6: 395– 397 [PubMed]
    [Google Scholar]
  23. Bernardet JF, Nakagawa Y, Holmes B. Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 2002; 52: 1049– 1070 [CrossRef] [PubMed]
    [Google Scholar]
  24. CLSI Performance Standards for Antimicrobial Disk Susceptibility Testing: Approved Standard CLSI Document M02-A11 Philadelphia, PA: Clinical and Laboratory Standards Institute; 2012
    [Google Scholar]
  25. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101. Newark, DE: MIDI Inc; 1990
    [Google Scholar]
  26. Minnikin DE, O'Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984; 2: 233– 241 [CrossRef]
    [Google Scholar]
  27. Collins MD. Analysis of isoprenoid quinones. In Gottschalk G. (editor) Methods in Microbiologyvol. 18 New York: Acad. Press; 1985; pp. 329– 366
    [Google Scholar]
  28. Minnikin DE, Patel PV, Alshamaony L, Goodfellow M. Polar lipid composition in the classification of Nocardia and related bacteria. Int J Syst Bacteriol 1977; 27: 104– 117 [CrossRef]
    [Google Scholar]
  29. Yoon JH, Lee SY. Winogradskyella multivorans sp. nov., a polysaccharide-degrading bacterium isolated from seawater of an oyster farm. Antonie van Leeuwenhoek 2012; 102: 231– 238 [CrossRef] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.002205
Loading
/content/journal/ijsem/10.1099/ijsem.0.002205
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error