1887

Abstract

To clarify the taxonomic classification of serotype 33, we performed biochemical and molecular genetic analyses using isolates (GUT-183, GUT-184, GUT-185, GUT-186, GUT-187, GUT-188, GUT-189, GUT-190, GUT-191, GUT-192 and GUT-193) from bovine endocarditis. A comparative sequence analysis showed 99.2–100 % sequence similarity among the reference strain of serotype 33 and our isolates for the 16S rRNA gene. These similarities were higher than those between the isolate GUT-187 and and other streptococci. Comparison of genes also showed high degrees of similarities among the reference strain of serotype 33 and our isolates (99.7–100 %), which were higher than those between the GUT-187 and and other streptococci. DNA–DNA relatedness among three isolates (GUT-186, GUT-187, the reference strain of serotype 33) was over 76.7 %. In contrast, the relatedness between GUT-187 and the other streptococcal species (, and ) was 8.4–24.9 %. Phylogenetic analyses showed that the isolates did not affiliate closely to any known species of the genus . Moreover, GUT-187 could be distinguished from and other closely related species of genus using biochemical tests. On the basis of the phenotypic and molecular genetic data, we propose that the isolates of serotype 33 should be classified into the genus , sp. nov. with the type strain GUT-187 (=DSM 104980=JCM 31869).

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2017-09-01
2024-03-29
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