1887

Abstract

To clarify the taxonomic classification of Streptococcus suis serotype 33, we performed biochemical and molecular genetic analyses using isolates (GUT-183, GUT-184, GUT-185, GUT-186, GUT-187, GUT-188, GUT-189, GUT-190, GUT-191, GUT-192 and GUT-193) from bovine endocarditis. A comparative sequence analysis showed 99.2–100 % sequence similarity among the reference strain of S. suis serotype 33 and our isolates for the 16S rRNA gene. These similarities were higher than those between the isolate GUT-187 and S. suis and other streptococci. Comparison of sodA genes also showed high degrees of similarities among the reference strain of S. suis serotype 33 and our isolates (99.7–100 %), which were higher than those between the GUT-187 and S. suis and other streptococci. DNA–DNA relatedness among three isolates (GUT-186, GUT-187, the reference strain of S. suis serotype 33) was over 76.7 %. In contrast, the relatedness between GUT-187 and the other streptococcal species ( S. suis , Streptococcus parasuis , Streptococcus acidominimus and Streptococcus porci ) was 8.4–24.9 %. Phylogenetic analyses showed that the isolates did not affiliate closely to any known species of the genus Streptococcus . Moreover, GUT-187 could be distinguished from S. suis and other closely related species of genus Streptococcus using biochemical tests. On the basis of the phenotypic and molecular genetic data, we propose that the isolates of S. suis serotype 33 should be classified into the genus Streptococcus , Streptococcus ruminantium sp. nov. with the type strain GUT-187 (=DSM 104980=JCM 31869).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.002204
2017-08-25
2019-10-16
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/67/9/3660.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.002204&mimeType=html&fmt=ahah

References

  1. Fittipaldi N, Segura M, Grenier D, Gottschalk M. Virulence factors involved in the pathogenesis of the infection caused by the swine pathogen and zoonotic agent Streptococcus suis. Future Microbiol 2012;7:259–279 [CrossRef][PubMed]
    [Google Scholar]
  2. Staats JJ, Feder I, Okwumabua O, Chengappa MM. Streptococcus suis: past and present. Vet Res Commun 1997;21:381–407 [CrossRef][PubMed]
    [Google Scholar]
  3. Wangsomboonsiri W, Luksananun T, Saksornchai S, Ketwong K, Sungkanuparph S. Streptococcus suis infection and risk factors for mortality. J Infect 2008;57:392–396 [CrossRef][PubMed]
    [Google Scholar]
  4. Mai NT, Hoa NT, Nga TV, Linh L, Chau TT et al. Streptococcus suis meningitis in adults in Vietnam. Clin Infect Dis 2008;46:659–667 [CrossRef][PubMed]
    [Google Scholar]
  5. Elliott SD. Streptococcal infection in young pigs. I. An immunochemical study of the causative agent (PM streptococcus). J Hyg 1966;64:205–212 [CrossRef][PubMed]
    [Google Scholar]
  6. Windsor RS, Elliott SD. Streptococcal infection in young pigs. IV. An outbreak of streptococcal meningitis in weaned pigs. J Hyg 1975;75:69–78 [CrossRef][PubMed]
    [Google Scholar]
  7. Perch B, Pedersen KB, Henrichsen J. Serology of capsulated streptococci pathogenic for pigs: six new serotypes of Streptococcus suis. J Clin Microbiol 1983;17:993–996[PubMed]
    [Google Scholar]
  8. Gottschalk M, Higgins R, Jacques M, Mittal KR, Henrichsen J. Description of 14 new capsular types of Streptococcus suis. J Clin Microbiol 1989;27:2633–2636[PubMed]
    [Google Scholar]
  9. Gottschalk M, Higgins R, Jacques M, Beaudoin M, Henrichsen J. Characterization of six new capsular types (23 through 28) of Streptococcus suis. J Clin Microbiol 1991;29:2590–2594[PubMed]
    [Google Scholar]
  10. Higgins R, Gottschalk M, Boudreau M, Lebrun A, Henrichsen J. Description of six new capsular types (29–34) of Streptococcus suis. J Vet Diagn Invest 1995;7:405–406 [CrossRef][PubMed]
    [Google Scholar]
  11. Liu Z, Zheng H, Gottschalk M, Bai X, Lan R et al. Development of multiplex PCR assays for the identification of the 33 serotypes of Streptococcus suis. PLoS One 2013;8:e72070 [CrossRef][PubMed]
    [Google Scholar]
  12. Athey TB, Teatero S, Lacouture S, Takamatsu D, Gottschalk M et al. Determining Streptococcus suis serotype from short-read whole-genome sequencing data. BMC Microbiol 2016;16:162 [CrossRef][PubMed]
    [Google Scholar]
  13. Hill JE, Gottschalk M, Brousseau R, Harel J, Hemmingsen SM et al. Biochemical analysis, cpn60 and 16S rDNA sequence data indicate that Streptococcus suis serotypes 32 and 34, isolated from pigs, are Streptococcus orisratti. Vet Microbiol 2005;107:63–69 [CrossRef][PubMed]
    [Google Scholar]
  14. Tien Leht, Nishibori T, Nishitani Y, Nomoto R, Osawa R. Reappraisal of the taxonomy of Streptococcus suis serotypes 20, 22, 26, and 33 based on DNA-DNA homology and sodA and recN phylogenies. Vet Microbiol 2013;162:842–849 [CrossRef][PubMed]
    [Google Scholar]
  15. Nomoto R, Maruyama F, Ishida S, Tohya M, Sekizaki T et al. Reappraisal of the taxonomy of Streptococcus suis serotypes 20, 22 and 26: Streptococcus parasuis sp. nov. Int J Syst Evol Microbiol 2015;65:438–443 [CrossRef][PubMed]
    [Google Scholar]
  16. Higgins R, Gottschalk M. An update on Streptococcus suis identification. J Vet Diagn Invest 1990;2:249–252 [CrossRef][PubMed]
    [Google Scholar]
  17. Glazunova OO, Raoult D, Roux V. Partial recN gene sequencing: a new tool for identification and phylogeny within the genus Streptococcus. Int J Syst Evol Microbiol 2010;60:2140–2148 [CrossRef][PubMed]
    [Google Scholar]
  18. Tien Leht, Sugiyama N, Duangsonk K, Tharavichitkul P, Osawa R. Phenotypic and PCR-based identification of bacterial strains isolated from patients with suspected Streptococcus suis infection in northern Thailand. Jpn J Infect Dis 2012;65:171–174[PubMed]
    [Google Scholar]
  19. Dorsch M, Stackebrandt E. Some modifications in the procedure of direct sequencing of PCR amplified 16S rDNA. J Microbiol Methods 1992;16:271–279 [CrossRef]
    [Google Scholar]
  20. Poyart C, Quesne G, Coulon S, Berche P, Trieu-Cuot P. Identification of streptococci to species level by sequencing the gene encoding the manganese-dependent superoxide dismutase. J Clin Microbiol 1998;36:41–47[PubMed]
    [Google Scholar]
  21. Tamura K, Peterson D, Peterson N, Stecher G, Nei M et al. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 2011;28:2731–2739[CrossRef]
    [Google Scholar]
  22. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987;4:406–425[PubMed]
    [Google Scholar]
  23. Ezaki T, Hashimoto Y, Yabuuchi E. Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 1989;39:224–229 [CrossRef]
    [Google Scholar]
  24. Tomida J, Sauer S, Morita Y, Ezaki T, Kawamura Y. Streptococcus fryi sp. nov., a novel species with Lancefield group M antigens. FEMS Microbiol Lett 2011;314:95–100 [CrossRef][PubMed]
    [Google Scholar]
  25. Wayne LG, Brenner DJ, Colwell RR, Grimont PAD, Kandler O et al. International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 1987;37:463–464[CrossRef]
    [Google Scholar]
  26. Mesbah M, Premachandran U, Whitman WB. Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 1989;39:159–167 [CrossRef]
    [Google Scholar]
  27. Facklam R, Elliott JA. Identification, classification, and clinical relevance of catalase-negative, gram-positive cocci, excluding the streptococci and enterococci. Clin Microbiol Rev 1995;8:479–495[PubMed]
    [Google Scholar]
  28. Vela AI, Casamayor A, Sánchez del Rey V, Domínguez L, Fernández-Garayzábal JF. Streptococcus plurextorum sp. nov., isolated from pigs. Int J Syst Evol Microbiol 2009;59:504–508 [CrossRef][PubMed]
    [Google Scholar]
  29. Vela AI, Perez M, Zamora L, Palacios L, Domínguez L et al. Streptococcus porci sp. nov., isolated from swine sources. Int J Syst Evol Microbiol 2010;60:104–108 [CrossRef][PubMed]
    [Google Scholar]
  30. Devriese LA, Vandamme P, Collins MD, Alvarez N, Pot B et al. Streptococcus pluranimalium sp. nov., from cattle and other animals. Int J Syst Bacteriol 1999;49:1221–1226 [CrossRef][PubMed]
    [Google Scholar]
  31. Vela AI, Fernández E, Lawson PA, Latre MV, Falsen E et al. Streptococcus entericus sp. nov., isolated from cattle intestine. Int J Syst Evol Microbiol 2002;52:665–669 [CrossRef][PubMed]
    [Google Scholar]
  32. Collins MD, Hutson RA, Falsen E, Inganäs E, Bisgaard M. Streptococcus gallinaceus sp. nov., from chickens. Int J Syst Evol Microbiol 2002;52:1161–1164 [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.002204
Loading
/content/journal/ijsem/10.1099/ijsem.0.002204
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error