1887

Abstract

A marine bacterium, named strain feces2, was isolated from the excreted faeces of an abalone, Haliotis discus hannai. The bacterium was Gram-stain-negative, rod-shaped and had a polar flagellum. It formed a white, small and crater-like colony on an agar plate, and had the capability of degrading agar. Activity of oxidase was positive and that of catalase was negative. Strain feces2 grew at 16 to 40 °C with an optimum of 28–30 °C. The nearly full-length 16S rRNA gene of strain feces2 had the greatest sequence similarity of 92.9 % with Marinibactrumhalimedae Q-192, followed by of 92.8 % with Teredinibacterturnerae T7902. Phylogenetic analysis indicated that strain feces2 belonged to the family Cellvibrionaceae , representing an independent clade with an uncultured bacterium clone NEP3-15 (98 % sequence similarity of 16S rRNA gene) derived from the phycosphere of Enteromorphaprolifera. The respiratory quinone was ubiquinone Q-8. The predominant fatty acids consisted of summed feature 8 (C18 : 1  ω7c/C18  : 1  ω6c), C16 : 0 and summed feature 3 (C16 : 1  ω6c/C16  : 1ω7c). The polar lipids were identified as phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, an unidentified amino phospholipid and four unknown lipids. The genomic DNA G+C content was 50.5 mol%. On the basis of polyphasic characterizations, strain feces2 represented a novel species and a novel genus in the family Cellvibrionaceae of the order Cellvibrionales within the Gammaproteobacteria , for which the name Agaribacteriumhaliotis gen. nov., sp. nov. is proposed, with the type strain being feces2 (=MCCC 1A11450=KCTC 52708).

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2017-09-13
2019-10-22
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References

  1. Zhang G, Que H, Liu X, Xu H. Abalone mariculture in China. J Shellfish Res 2004; 23: 947– 950
    [Google Scholar]
  2. Huang Z-B, Guo F, Zhao J, Li W-D, Ke C-H. Molecular analysis of the intestinal bacterial flora in cage-cultured adult small abalone, Haliotis diversicolor. Aquacult Res 2010; 41: e760 e769 [CrossRef]
    [Google Scholar]
  3. Erasmus JH, Cook PA, Coyne VE. The role of bacteria in the digestion of seaweed by the abalone Haliotis midae. Aquaculture 1997; 155: 377– 386 [CrossRef]
    [Google Scholar]
  4. Sawabe T, Oda Y, Shiomi Y, Ezura Y. Alginate degradation by bacteria isolated from the gut of sea urchins and abalones. Microb Ecol 1995; 30: 193– 202 [CrossRef] [PubMed]
    [Google Scholar]
  5. Spring S, Scheuner C, Göker M, Klenk HP. A taxonomic framework for emerging groups of ecologically important marine gammaproteobacteria based on the reconstruction of evolutionary relationships using genome-scale data. Front Microbiol 2015; 6: 281 [CrossRef] [PubMed]
    [Google Scholar]
  6. Yoon SH, Ha SM, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017; 67: 1613– 1617 [CrossRef] [PubMed]
    [Google Scholar]
  7. Yarza P, Yilmaz P, Pruesse E, Glöckner FO, Ludwig W et al. Uniting the classification of cultured and uncultured bacteria and archaea using 16S rRNA gene sequences. Nat Rev Microbiol 2014; 12: 635– 645 [CrossRef] [PubMed]
    [Google Scholar]
  8. Delong EF. Archaea in coastal marine environments. Proc Natl Acad Sci USA 1992; 89: 5685– 5689 [CrossRef] [PubMed]
    [Google Scholar]
  9. Kim OS, Cho YJ, Lee K, Yoon SH, Kim M et al. Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 2012; 62: 716– 721 [CrossRef] [PubMed]
    [Google Scholar]
  10. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 2016; 33: 1870– 1874 [CrossRef] [PubMed]
    [Google Scholar]
  11. Bankevich A, Nurk S, Antipov D, Gurevich AA, Dvorkin M et al. SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing. J Comput Biol 2012; 19: 455– 477 [CrossRef] [PubMed]
    [Google Scholar]
  12. Gurevich A, Saveliev V, Vyahhi N, Tesler G. QUAST: quality assessment tool for genome assemblies. Bioinformatics 2013; 29: 1072– 1075 [CrossRef] [PubMed]
    [Google Scholar]
  13. Komagata K, Suzuki K. Lipid and cell-wall analysis in bacterial systematics. Method Microbiol 1987; 19: 161– 207 [Crossref]
    [Google Scholar]
  14. Urios L, Intertaglia L, Lesongeur F, Lebaron P. Eionea nigra gen. nov., sp. nov., a gammaproteobacterium from the Mediterranean Sea. Int J Syst Evol Microbiol 2011; 61: 1677– 1681 [CrossRef] [PubMed]
    [Google Scholar]
  15. Baek K, Choi A, Cho JC. Eionea flava sp. nov., isolated from coastal seawater, and emended description of the genus Eionea. Int J Syst Evol Microbiol 2015; 65: 2975– 2979 [CrossRef] [PubMed]
    [Google Scholar]
  16. Nishijima M, Adachi K, Sano H, Yamasato K. Marinibactrum halimedae gen. nov., sp. nov., a novel gammaproteobacterium isolated from a marine macroalga. Int J Syst Evol Microbiol 2015; 66: 3866– 3871 [Crossref]
    [Google Scholar]
  17. Distel DL, Morrill W, Maclaren-Toussaint N, Franks D, Waterbury J. Teredinibacter turnerae gen. nov., sp. nov., a dinitrogen-fixing, cellulolytic, endosymbiotic gamma-proteobacterium isolated from the gills of wood-boring molluscs (Bivalvia: Teredinidae). Int J Syst Evol Microbiol 2002; 52: 2261– 2269 [CrossRef] [PubMed]
    [Google Scholar]
  18. Chen MH, Sheu SY, Arun AB, Young CC, Chen CA et al. Pseudoteredinibacter isoporae gen. nov., sp. nov., a marine bacterium isolated from the reef-building coral Isopora palifera. Int J Syst Evol Microbiol 2011; 61: 1887– 1893 [CrossRef] [PubMed]
    [Google Scholar]
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