1887

Abstract

A Gram-stain-negative, coccoid, yellow, non-motile, aerobic bacterium, designated strain S36, was isolated from soil of the Xixi wetland in Zhejiang province, PR China. Phylogenetic analysis, based on 16S rRNA gene sequences, revealed that strain S36 could represent a novel species of genus showing highest similarity to 26DY36 (96.31 % 16S rRNA gene sequence similarity). The temperature, pH and NaCl concentration ranges for growth were 10–37 °C (optimum 32 °C), pH 5.0–10.0 (optimum pH 7.0) and 0.5–3 % (optimum 1 %, w/v), respectively. The predominant respiratory quinone of strain S36 was Q-10. The major fatty acids were C, Cω, Cω and summed feature 3 (Cω and/or iso-C 2-OH). The G+C content of the genomic DNA was 62.7 mol%. These data all support the affiliation of strain S36 to the genus . The polar lipids profile of strain S36 comprised diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, two unidentified phospholipids and two unidentified glycolipids. The results of physiological and biochemical tests allowed differentiation of strain S36 from other members of the genus . Therefore, strain S36 represents a novel species of the genus , for which the name sp. nov. is proposed; the type strain is S36 (=CGMCC 1.12804=NBRC 110413).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.002198
2017-09-01
2020-01-23
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/67/9/3655.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.002198&mimeType=html&fmt=ahah

References

  1. Kwon KK, Woo JH, Yang SH, Kang JH, Kang SG et al. Altererythrobacter epoxidivorans gen. nov., sp. nov., an epoxide hydrolase-active, mesophilic marine bacterium isolated from cold-seep sediment, and reclassification of Erythrobacter luteolus Yoon et al. 2005 as Altererythrobacter luteolus comb. nov. Int J Syst Evol Microbiol 2007;57:2207–2211 [CrossRef][PubMed]
    [Google Scholar]
  2. Xue X, Zhang K, Cai F, Dai J, Wang Y et al. Altererythrobacter xinjiangensis sp. nov., isolated from desert sand, and emended description of the genus Altererythrobacter. Int J Syst Evol Microbiol 2012;62:28–32 [CrossRef][PubMed]
    [Google Scholar]
  3. Lai Q, Yuan J, Shao Z. Altererythrobacter marinus sp. nov., isolated from deep seawater. Int J Syst Evol Microbiol 2009;59:2973–2976 [CrossRef][PubMed]
    [Google Scholar]
  4. Seo SH, Lee SD. Altererythrobacter marensis sp. nov., isolated from seawater. Int J Syst Evol Microbiol 2010;60:307–311 [CrossRef][PubMed]
    [Google Scholar]
  5. Park SC, Baik KS, Choe HN, Lim CH, Kim HJ et al. Altererythrobacter namhicola sp. nov. and Altererythrobacter aestuarii sp. nov., isolated from seawater. Int J Syst Evol Microbiol 2011;61:709–715 [CrossRef][PubMed]
    [Google Scholar]
  6. Matsumoto M, Iwama D, Arakaki A, Tanaka A, Tanaka T et al. Altererythrobacter ishigakiensis sp. nov., an astaxanthin-producing bacterium isolated from a marine sediment. Int J Syst Evol Microbiol 2011;61:2956–2961 [CrossRef][PubMed]
    [Google Scholar]
  7. Kumar NR, Nair S, Langer S, Busse HJ, Kämpfer P et al. Altererythrobacter indicus sp. nov., isolated from wild rice (Porteresia coarctata Tateoka). Int J Syst Evol Microbiol 2008;58:839–844 [CrossRef][PubMed]
    [Google Scholar]
  8. Yoon JH, Kang KH, Yeo SH, Oh TK. Erythrobacter luteolus sp. nov., isolated from a tidal flat of the Yellow Sea in Korea. Int J Syst Evol Microbiol 2005;55:1167–1170 [CrossRef][PubMed]
    [Google Scholar]
  9. Fan ZY, Xiao YP, Hui W, Tian GR, Lee JS et al. Altererythrobacter dongtanensis sp. nov., isolated from a tidal flat. Int J Syst Evol Microbiol 2011;61:2035–2039 [CrossRef][PubMed]
    [Google Scholar]
  10. Jeong SH, Jin HM, Lee HJ, Jeon CO. Altererythrobacter gangjinensis sp. nov., a marine bacterium isolated from a tidal flat. Int J Syst Evol Microbiol 2013;63:971–976 [CrossRef][PubMed]
    [Google Scholar]
  11. Nedashkovskaya OI, Cho SH, Joung Y, Joh K, Kim MN et al. Altererythrobacter troitsensis sp. nov., isolated from the sea urchin Strongylocentrotus intermedius. Int J Syst Evol Microbiol 2013;63:93–97 [CrossRef][PubMed]
    [Google Scholar]
  12. Huang YL, Ki JS, Case RJ, Qian P. Diversity and acyl-homoserine lactone production among subtidal biofilm-forming bacteria. Aquat Microb Ecol 2008;52:185–193 [CrossRef]
    [Google Scholar]
  13. Kim OS, Cho YJ, Lee K, Yoon SH, Kim M et al. Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 2012;62:716–721 [CrossRef][PubMed]
    [Google Scholar]
  14. Thompson JD, Higgins DG, Gibson TJ. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 1994;22:4673–4680 [CrossRef][PubMed]
    [Google Scholar]
  15. Hall TA. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 1999;41:95–98
    [Google Scholar]
  16. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987;4:406–425[PubMed]
    [Google Scholar]
  17. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981;17:368–376 [CrossRef][PubMed]
    [Google Scholar]
  18. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013;30:2725–2729 [CrossRef][PubMed]
    [Google Scholar]
  19. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985;39:783–791 [CrossRef][PubMed]
    [Google Scholar]
  20. Kimura M. The Neutral Theory of Molecular Evolution Cambridge, UK: Cambridge University Press; 1983;[CrossRef]
    [Google Scholar]
  21. Huo YY, Xu XW, Cui HL, Wu M. Gracilibacillus ureilyticus sp. nov., a halotolerant bacterium from a saline-alkaline soil. Int J Syst Evol Microbiol 2010;60:1383–1386 [CrossRef][PubMed]
    [Google Scholar]
  22. Dong X, Cai M. Common Manual of Systematic Bacteriology Beijing: Science Press; 2001
    [Google Scholar]
  23. Minnikin DE, O'Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984;2:233–241 [CrossRef]
    [Google Scholar]
  24. Sasser M. Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101 Newark, DE: MIDI Inc; 1990
    [Google Scholar]
  25. Komagata K, Suzuki K. Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 1987;19:161–207[CrossRef]
    [Google Scholar]
  26. Mesbah M, Whitman WB. Measurement of deoxyguanosine/thymidine ratios in complex mixtures by high-performance liquid chromatography for determination of the mole percentage guanine + cytosine of DNA. J Chromatogr 1989;479:297–306 [CrossRef][PubMed]
    [Google Scholar]
  27. Wu YH, Xu L, Meng FX, Zhang DS, Wang CS et al. Altererythrobacter atlanticus sp. nov., isolated from deep-sea sediment. Int J Syst Evol Microbiol 2014;64:116–121 [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.002198
Loading
/content/journal/ijsem/10.1099/ijsem.0.002198
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error