%0 Journal Article %A Tohno, Masanori %A Tanizawa, Yasuhiro %A Irisawa, Tomohiro %A Masuda, Takaharu %A Sakamoto, Mitsuo %A Arita, Masanori %A Ohkuma, Moriya %A Kobayashi, Hisami %T Lactobacillus silagincola sp. nov. and Lactobacillus pentosiphilus sp. nov., isolated from silage %D 2017 %J International Journal of Systematic and Evolutionary Microbiology, %V 67 %N 9 %P 3639-3644 %@ 1466-5034 %R https://doi.org/10.1099/ijsem.0.002196 %K Lactobacillus pentosiphilus sp. nov. %K silage %K Lactobacillus silagincola sp. nov. %I Microbiology Society, %X Three Gram-stain positive, non-motile, non-spore-forming, catalase-negative and rod-shaped bacterial strains (IWT5T, IWT25T and IWT140), isolated from silage, were investigated by using a polyphasic taxonomic approach. Strains IWT5T and IWT25T grew at 10–37 °C and 30–37 °C, and at pH 4.0–7.5 and 4.0–7.0, respectively. The G+C contents of genomic DNA of strains IWT5T and IWT25T were 43.2 and 44.4 mol%, respectively. Strains IWT5T and IWT25T contained C16 : 0, C18 : 1 ω9c and summed feature 7 (unknown 18.846/C19 : 1 ω6c/C19  : 0cyclo ω10c) as the major fatty acids. Strain IWT5T was most closely related to the type strains of Lactobacillus mixtipabuli (99.9 % 16S rRNA gene sequence similarity) and Lactobacillus silagei (99.5 %). For IWT25T, the 16S rRNA gene sequence similarities with the closely related neighbour type strains L. mixtipabuli and L. silagei were 99.5 and 99.5 %, respectively. The 16S rRNA gene sequence similarities among the three novel isolates were 99.5–99.9 %. The average nucleotide identities of strains IWT5T and IWT25T to other neighbours of the genus Lactobacillus were less than 82 % and the genomes of IWT25T and IWT140 shared 97.3 % average nucleotide identity, demonstrating that the three strains were allocated to two different novel species of the genus Lactobacillus . Together with multilocus sequence analysis, phenotypic and chemotaxonomic characteristics, strains IWT5T (=JCM 31144T=DSM 102973T) and IWT25T (=JCM 31145T=DSM 102974T) are proposed as the type strains of novel species of the genus Lactobacillus , with the names Lactobacillus silagincola sp. nov. and Lactobacillus pentosiphilus sp. nov., respectively. %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002196