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Abstract

Three Gram-stain positive, non-motile, non-spore-forming, catalase-negative and rod-shaped bacterial strains (IWT5, IWT25 and IWT140), isolated from silage, were investigated by using a polyphasic taxonomic approach. Strains IWT5 and IWT25 grew at 10–37 °C and 30–37 °C, and at pH 4.0–7.5 and 4.0–7.0, respectively. The G+C contents of genomic DNA of strains IWT5 and IWT25 were 43.2 and 44.4 mol%, respectively. Strains IWT5 and IWT25 contained C, C ω and summed feature 7 (unknown 18.846/C ω6/ cyclo ω10) as the major fatty acids. Strain IWT5 was most closely related to the type strains of (99.9 % 16S rRNA gene sequence similarity) and (99.5 %). For IWT25, the 16S rRNA gene sequence similarities with the closely related neighbour type strains and were 99.5 and 99.5 %, respectively. The 16S rRNA gene sequence similarities among the three novel isolates were 99.5–99.9 %. The average nucleotide identities of strains IWT5 and IWT25 to other neighbours of the genus were less than 82 % and the genomes of IWT25 and IWT140 shared 97.3 % average nucleotide identity, demonstrating that the three strains were allocated to two different novel species of the genus . Together with multilocus sequence analysis, phenotypic and chemotaxonomic characteristics, strains IWT5 (=JCM 31144=DSM 102973) and IWT25 (=JCM 31145=DSM 102974) are proposed as the type strains of novel species of the genus , with the names sp. nov. and sp. nov., respectively.

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2017-09-01
2020-01-27
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